In biological study, one sometimes needs to detect the simultaneous presence of a micro RNA (miRNA) and its putative target mRNA in the same cell. As no such technique is unavailable, Dr. Wen-Hsiung Li’s team at Biodiversity Research Center develop a in situ method for this purpose. It uses rolling-circle amplification of RNAs to achieve a high signal/noise ratio. It can be applied to simultaneous detection of multiple RNAs in the same cell. The results were published in Plant Biotechnology Journal.

For further information: https://www.biodiv.tw/research-20221110155931
Article link: https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.13931