Wild tomato (Solanum peruvianum) is an important genetic source for the tomato breeding, but the progress has been hampered due to the self-incompatibility and highly heterozygous genome. Yao-Cheng Lin, Choun-Sea Lin, and Ming-Che Shih from Agricultural Biotechnology Research Center developed a DNA-free CRISPR-Cas9 genome editing tool based on an optimized protoplast regeneration protocol that creates stable and inheritable diploid and tetraploid regenerants. The study was published in Plant Physiology and selected as the cover image for the April Issue.

Article link：https://doi.org/10.1093/plphys/kiac022