{"id":21747,"date":"2020-04-23T00:03:32","date_gmt":"2020-04-22T16:03:32","guid":{"rendered":"https:\/newsletter.sinica.edu.tw\/?p=21747"},"modified":"2021-08-26T09:07:29","modified_gmt":"2021-08-26T01:07:29","slug":"%e3%80%90%e5%b0%88%e6%ac%84%e3%80%91%e8%b6%85%e9%ab%98%e8%a7%a3%e6%9e%90%e5%ba%a6%e6%87%89%e7%94%a8%ef%bc%9a%e4%b8%ad%e5%bf%83%e7%b2%92%e8%88%87%e4%b8%bb%e7%ba%96%e6%af%9b-%e2%94%80","status":"publish","type":"post","link":"https:\/newsletter.sinica.edu.tw\/21747\/","title":{"rendered":"\u3010\u5c08\u6b04\u3011\u8d85\u9ad8\u89e3\u6790\u5ea6\u61c9\u7528\uff1a\u4e2d\u5fc3\u7c92\u8207\u4e3b\u7e96\u6bdb \u2500\u2500\u9023\u63a5\u7d50\u69cb\u751f\u7269\u5b78\u8207\u7d30\u80de\u751f\u7269\u5b78\u7684\u6a4b\u6a11"},"content":{"rendered":"

\u4f5c\u8005\uff1a\u5ed6\u4ef2\u9e92\u7814\u7a76\u54e1\uff08\u672c\u9662\u539f\u5b50\u8207\u5206\u5b50\u79d1\u5b78\u7814\u7a76\u6240\uff09<\/p>\n

\u52d5\u7269\u7d30\u80de\u5206\u88c2\u7684\u6642\u5019\uff0c\u6709\u4e00\u7d44\u5c0f\u7684\u7d50\u69cb\u4e5f\u6703\u8ddf\u8457\u7d30\u80de\u4e00\u8d77\u8907\u88fd\uff0c\u518d\u5206\u5230\u5169\u500b\u7d30\u80de\u4e2d\u3002\u9019\u4e00\u7d44\u5c0f\u7d50\u69cb\u5305\u542b\u5169\u500b\u5713\u67f1\u5f62\u7684\u7d50\u69cb\uff0c\u7a31\u4e4b\u70ba\u4e2d\u5fc3\u7c92\u3002\u70ba\u4ec0\u9ebc\u9700\u8981\u9019\u500b\u4e2d\u5fc3\u7c92\u8ddf\u8457\u7d30\u80de\u4e00\u8d77\u5206\u88c2\u4e00\u8d77\u5206\u914d\u5230\u5169\u500b\u7d30\u80de\u5462\uff1f\u70ba\u4ec0\u9ebc\u4e2d\u5fc3\u7c92\u7684\u5f62\u72c0\u4e0d\u662f\u5176\u4ed6\u5f62\u72c0\u800c\u662f\u5f88\u7279\u5225\u7684\u67f1\u72c0\u7d50\u69cb\u5462\uff1f<\/p>\n

\u8fd1\u671f\u7684\u7814\u7a76\u767c\u73fe\uff0c\u4e2d\u5fc3\u7c92\u9664\u4e86\u8ddf\u8457\u7d30\u80de\u5206\u88c2\u64d4\u4efb\u62c9\u8457\u5169\u7aefDNA\u5206\u958b\u7684\u5de5\u4f5c\u4e4b\u5916\uff0c\u9084\u8ca0\u8cac\u4e86\u9577\u4e00\u6839\u53eb\u505a\u4e3b\u7e96\u6bdb\u7684\u7d50\u69cb\u3002\u9019\u4e3b\u7e96\u6bdb\u7684\u7d50\u69cb\u4e5f\u662f\u4e00\u500b\u9577\u7684\u5713\u67f1\u72c0\u7684\u7d50\u69cb\uff0c\u56e0\u6b64\u4e2d\u5fc3\u7c92\u9577\u6210\u67f1\u72c0\uff0c\u4e8b\u5be6\u4e0a\u662f\u8ca0\u8cac\u9019\u6700\u91cd\u8981\u7684\u4e8b\u60c5\uff0c\u4e5f\u5c31\u662f\u7576\u4f5c\u4e3b\u7e96\u6bdb\u7684\u57fa\u5e95\u3002\u4e3b\u7e96\u6bdb\u8ca0\u8cac\u7d30\u80de\u7684\u4e00\u4e9b\u91cd\u8981\u7684\u611f\u61c9\uff0c\u7279\u5225\u662f\u4e0a\u9762\u6709\u5f88\u591a\u63a5\u53d7\u5668\u53ef\u4ee5\u5075\u6e2c\u9644\u8fd1\u4e00\u4e9b\u91cd\u8981\u7684\u5206\u5b50\uff0c\u800c\u9032\u4e00\u6b65\u544a\u8a34\u7d30\u80de\u8a72\u505a\u4ec0\u9ebc\u3002\u4f8b\u5982\u5f88\u91cd\u8981\u7684hedgehog signaling \uff0c\u5c31\u662f\u6211\u5011\u5728\u767c\u80b2\u7684\u6642\u5019\u4e00\u500b\u975e\u5e38\u91cd\u8981\u7684\uff0c\u6c7a\u5b9a\u6211\u5011\u5fc3\u81df\u9577\u54ea\u4e00\u908a\uff0c\u624b\u6307\u6709\u5e7e\u6839\u7684\u91cd\u8981\u8abf\u63a7\u3002<\/p>\n

\u9019\u500b\u4e3b\u7e96\u6bdb\u70ba\u4e86\u8981\u505a\u9019\u6a23\u7684\u4e8b\u60c5\uff0c\u5fc5\u9808\u63a7\u5236\u4e3b\u7e96\u6bdb\u5167\u90e8\u7279\u6b8a\u7684\u86cb\u767d\u8cea\u7684\u5206\u4f48\uff0c\u4e0d\u8a72\u9032\u53bb\u7684\u86cb\u767d\u8cea\u5c31\u4e0d\u80fd\u9032\u53bb\u9019\u500b\u4e3b\u7e96\u6bdb\uff0c\u800c\u8a72\u9032\u53bb\u7684\u7531\u4e3b\u7e96\u6bdb\u5e95\u90e8\u7684\u7d50\u69cb\u8abf\u63a7\uff0c\u50cf\u4e00\u500b\u5b88\u9580\u54e1\u4e00\u6a23\u3002\u5728\u9019\u500b\u4e3b\u7e96\u6bdb\u5e95\u90e8\u4e5f\u5c31\u662f\u5728\u4e2d\u5fc3\u7c92\u7684\u6700\u9802\u7aef\uff0c\u5305\u542b\u4e86\u975e\u5e38\u591a\u91cd\u8981\u7684\u86cb\u767d\u8cea\u3002\u9019\u4e9b\u86cb\u767d\u8cea\u9664\u4e86\u8ca0\u8cac\u8abf\u63a7\u7576\u5b88\u9580\u54e1\u4e4b\u5916\uff0c\u53e6\u5916\u4e5f\u5305\u542b\u4e86\u628a\u6574\u6839\u4e3b\u7e96\u6bdb\u652f\u6490\u8d77\u4f86\uff0c\u4ee5\u53ca\u64d4\u4efb\u7d30\u80de\u4e2d\u5fae\u7ba1\u805a\u96c6\u7684\u4e00\u500b\u91cd\u8981\u90e8\u4f4d\u3002\u5f9e\u96fb\u5b50\u986f\u5fae\u93e1\u53ef\u4ee5\u770b\u5230\uff0c\u5728\u9019\u4e2d\u5fc3\u7c92\u9802\u90e8\u9644\u8fd1\uff0c\u6709\u4e0d\u5c11\u7684\u5fae\u7ba1\u76f4\u63a5\u8cbc\u5728\u9019\u500b\u90e8\u5206\uff0c\u4e5f\u8a31\u5728\u4e3b\u7e96\u6bdb\u5167\u6536\u5230\u7684\u8cc7\u8a0a\uff0c\u53ef\u4ee5\u8fc5\u901f\u7684\u7d93\u7531\u9019\u4e9b\u5fae\u7ba1\u806f\u7d61\u7d30\u80de\u7684\u5404\u90e8\u5206\uff0c\u8b93\u7d30\u80de\u53ef\u4ee5\u505a\u5168\u9762\u6027\u7684\u53cd\u61c9\u3002\u90a3\u9ebc\uff0c\u5728\u9019\u500b\u4e3b\u7e96\u6bdb\u5e95\u90e8\u4e2d\u5fc3\u7c92\u9802\u90e8\uff0c\u5230\u5e95\u6709\u54ea\u4e9b\u91cd\u8981\u7684\u7d50\u69cb\u53ca\u86cb\u767d\u8cea\u5462\uff1f\u4ed6\u5011\u5206\u5225\u7684\u529f\u80fd\u53c8\u662f\u4ec0\u9ebc\u5462\uff1f\u4ed6\u5011\u7684\u7d50\u69cb\u5230\u5e95\u9577\u4ec0\u9ebc\u6a23\u5b50\u5462\uff1f\u5176\u5be6\u90fd\u662f\u751f\u7269\u5b78\u5bb6\u975e\u5e38\u60f3\u8981\u77e5\u9053\u7684\u3002<\/p>\n

\u5728\u4e3b\u7e96\u6bdb\u5e95\u90e8\u6709\u4e00\u500b\u7d50\u69cb\u53eb\u505a\u904e\u6e21\u5340\uff0c\u9019\u500b\u7d50\u69cb\u8ca0\u8cac\u4e86\u4e0a\u9762\u6240\u8aaa\u7684\u8abf\u63a7\u7e96\u6bdb\u86cb\u767d\u8cea\u9032\u51fa\u7684\u529f\u80fd\u3002\u5728\u9019\u500b\u904e\u6e21\u5340\u7684\u90e8\u5206\uff0c\u4eba\u5011\u5df2\u7d93\u767c\u73fe\u5f88\u591a\u91cd\u8981\u7684\u86cb\u767d\u8cea\uff0c\u5305\u62ec\u6578\u500b\u8207\u6240\u8b02\u7684\u7e96\u6bdb\u76f8\u95dc\u75be\u75c5ciliopathy\u6709\u5bc6\u5207\u7684\u95dc\u4fc2\u7684\u86cb\u767d\u8cea\uff0c\u53ea\u8981\u9019\u908a\u7684\u86cb\u767d\u8cea\u6709\u7a81\u8b8a\uff0c\u5c31\u6703\u9020\u6210\u56b4\u91cd\u7684\u7e96\u6bdb\u6027\u75be\u75c5\uff0c\u75c5\u4eba\u5e38\u5e38\u8f03\u77ed\u58fd\u6216\u6709\u56b4\u91cd\u7684\u8996\u529b\u814e\u81df\u554f\u984c\uff0c\u56e0\u70ba\u7e96\u6bdb\u4e3b\u8981\u7684\u529f\u80fd\u6703\u88ab\u56b4\u91cd\u7684\u7834\u58de\u3002\u5728\u904e\u6e21\u5340\u7684\u4e0b\u9762\u6709\u53e6\u5916\u4e00\u500b\u91cd\u8981\u7684\u7d50\u69cb\uff0c\u9019\u500b\u7d50\u69cb\u5728\u96fb\u5b50\u986f\u5fae\u93e1\u4e0b\u53ef\u4ee5\u6e05\u695a\u7684\u770b\u5230\u662f9\u500b\u8f3b\u5c04\u51fa\u53bb\u7684\u77ed\u68d2\uff0c\u9019\u500b\u7d50\u69cb\u53eb\u505a\u9060\u7aef\u9644\u5c6c\u7269\u3002\u9060\u7aef\u9644\u5c6c\u7269\u7684\u90199\u500b\u50cf\u6247\u8449\u822c\u7684\u7d50\u69cb\u4e3b\u8981\u7684\u529f\u80fd\u5c31\u662f\u9023\u7d50\u7e96\u6bdb\u5e95\u90e8\u7684\u819c\uff0c\u652f\u6490\u8457\u6574\u500b\u4e3b\u7e96\u6bdb\u3002<\/p>\n

\u5728\u9019\u500b\u9060\u7aef\u9644\u5c6c\u7269\u4e4b\u4e0b\uff0c\u53e6\u5916\u9084\u6709\u4e00\u500b\u7d50\u69cb\uff0c\u53eb\u505a\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u3002\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u5728\u96fb\u5b50\u986f\u5fae\u93e1\u4e0b\uff0c\u770b\u4f3c\u5e7e\u500b\u4e09\u89d2\u5f62\u7684\u7d50\u69cb\u74b0\u7e5e\u5728\u4e2d\u5fc3\u7c92\u5468\u570d\uff0c\u6709\u6642\u4f3c\u4e4e\u67099\u500b\uff0c\u6709\u6642\u4f3c\u4e4e\u6bd4\u8f03\u5c11\u3002\u5e38\u5e38\u53ef\u4ee5\u770b\u5230\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u5728\u5b83\u5011\u4e09\u89d2\u5f62\u7d50\u69cb\u7684\u5c16\u7aef\u9023\u7d50\u8457\u5fae\u7ba1\u3002\u5fae\u7ba1\u5e38\u5e38\u5f80\u5916\u5ef6\u4f38\uff0c\u4f3c\u4e4e\u6709\u7d50\u69cb\u652f\u6490\u548c\u5f80\u5916\u806f\u7e6b\u7684\u529f\u80fd\u3002\u9019\u4e9b\u7d50\u69cb\u57fa\u672c\u4e0a\u96d6\u7136\u5728\u96fb\u5b50\u986f\u5fae\u93e1\u4e0b\u770b\u5f97\u5230\uff0c\u4f46\u662f\u5404\u81ea\u5305\u542b\u5f88\u591a\u7684\u86cb\u767d\u8cea\u3002\u4e00\u822c\u6c92\u6709\u8fa6\u6cd5\u6e05\u6e05\u695a\u695a\u7684\u770b\u51fa\u9019\u4e9b\u86cb\u767d\u8cea\u5b83\u5011\u7684\u76f8\u5c0d\u4f4d\u7f6e\uff0c\u56e0\u70ba\u9019\u4e9b\u7d50\u69cb\u57fa\u672c\u4e0a\u90fd\u570d\u7e5e\u5728\u76f4\u5f91\u5927\u7d04180\u5948\u7c73\u7684\u4e2d\u5fc3\u7c92\u548c\u4e3b\u7e96\u6bdb\u7684\u5468\u570d\u3002\u4ee5\u4e00\u822c\u5149\u5b78\u986f\u5fae\u93e1\u7684\u89e3\u6790\u5ea6\u5927\u7d04\u53ef\u4ee5\u89e3\u6790250\u5948\u7c73\uff0c\u5728\u9019\u500b180\u5948\u7c73\u5468\u570d\u5927\u5bb6\u7684\u76f8\u5c0d\u4f4d\u7f6e\u751a\u81f3\u5c0f\u65bc100\u5948\u7c73\uff0c\u4e00\u822c\u7684\u5149\u5b78\u986f\u5fae\u93e1\u662f\u6c92\u8fa6\u6cd5\u5206\u8fa8\u7684\u3002<\/p>\n

\"\"

\u5716\u4e00 \u6b21\u9060\u7aef\u9644\u5c6c\u7269\u7684\u8d85\u9ad8\u89e3\u6790\u5ea6\u5f71\u50cf\uff0c\u6bd4\u4f8b\u5c3a200\u5948\u7c73<\/p><\/div>\n

\u6240\u4ee5\u61c9\u8a72\u600e\u9ebc\u8fa6\u624d\u80fd\u770b\u5230\u4ed6\u5011\u9019\u4e9b\u86cb\u767d\u8cea\u4e92\u76f8\u7684\u4f4d\u7f6e\uff0c\u7136\u5f8c\u4e86\u89e3\u4ed6\u5011\u5404\u81ea\u5728\u90a3\u500b\u4f4d\u7f6e\u53ef\u80fd\u7684\u529f\u80fd\u5462\uff1f\u5f9e2001\u5e74\u958b\u59cb\uff0c\u6709\u5e7e\u500b\u91cd\u8981\u7684\u5149\u5b78\u7a81\u7834\uff0c\u8b93\u672c\u4f86\u4e00\u822c\u5149\u5b78\u986f\u5fae\u93e1\u53ea\u80fd\u9054\u5230250\u5948\u7c73\u5de6\u53f3\u89e3\u6790\u5ea6\u7684\u9650\u5236\u53ef\u4ee5\u6253\u7834\uff0c\u5176\u4e2d\u5305\u62ecStefan Hell\u767c\u660e\u7684STED\u8d85\u9ad8\u89e3\u6790\u5ea6\u986f\u5fae\u8853\u4ee5\u53caEric Betzig\u767c\u660e\u7684PALM\u8d85\u9ad8\u89e3\u6790\u5ea6\u986f\u5fae\u8853\uff0c\u5c07\u4eba\u985e\u5149\u5b78\u986f\u5fae\u93e1\u7684\u89e3\u6790\u5ea6\u5927\u5e45\u63d0\u5347\u3002\u9019\u4e9b\u5149\u5b78\u7684\u7a81\u7834\u8b93\u8a31\u591a\u672c\u4f86\u4e0d\u53ef\u80fd\u7684\u7d50\u69cb\u89e3\u6790\u8b8a\u70ba\u53ef\u80fd\uff0c\u4e5f\u56e0\u6b64\u7372\u5f97\u4e862014\u5e74\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u3002STED\u5de7\u5999\u5730\u7d50\u5408\u4e86\u5169\u500b\u65b9\u6cd5\uff0c\u5176\u4e2d\u4e00\u500b\u662f\u5229\u7528stimulated emission\uff0c\u4e5f\u5c31\u662f\u96f7\u5c04LASER\u7684SE\uff0c\u5c07\u5206\u5b50\u5f9e\u6fc0\u767c\u614b\u5f37\u5236\u91cb\u653e\u5149\u5b50\uff0c\u8b93\u5206\u5b50\u9084\u4f86\u4e0d\u53ca\u91cb\u653e\u87a2\u5149\u524d\u5c31\u56de\u5230\u57fa\u614b\uff0c\u85c9\u7531\u6ffe\u7247\u6ffe\u6389\u5f8c\u9019\u4e9b\u5206\u5b50\u770b\u8d77\u4f86\u5c31\u662f\u6c92\u6709\u87a2\u5149\u8a0a\u865f\u7684\u3002\u53e6\u4e00\u500b\u65b9\u6cd5\u5c31\u662f\u904b\u7528\u7279\u6b8a\u7684\u5149\u5b78\u6e26\u65cb\u76f8\u4f4d\u677f\uff0c\u5c07\u6b64\u96f7\u5c04\u7684\u5206\u4f48\u8f49\u70ba\u74b0\u72c0\u7684\u5206\u4f48\uff0c\u56e0\u6b64\u5728\u74b0\u72c0\u7684\u5340\u57df\u5927\u90e8\u5206\u7684\u5206\u5b50\u6c92\u6709\u8fa6\u6cd5\u91cb\u653e\u87a2\u5149\uff0c\u4e2d\u9593\u7684\u5340\u57df\u4ecd\u7136\u91cb\u653e\u87a2\u5149\uff0c\u4e5f\u5c31\u662f\u8aaa\uff0c\u53ef\u4ee5\u628a\u5169\u500b\u5340\u57df\u7684\u5206\u5b50\u5176\u5be6\u5728\u8ddd\u96e2\u5f88\u8fd1\u7684\u72c0\u6cc1\u4e0b\u5206\u8fa8\u51fa\u4f86\uff0c\u9054\u5230\u8d85\u9ad8\u89e3\u6790\u5ea6\u7684\u986f\u5fae\u80fd\u529b\u3002\u63a7\u5236\u96f7\u5c04\u7684\u5f37\u5ea6\u4e0bSTED\uff0c\u53ef\u4ee5\u9054\u523050\u5948\u7c73\u7684\u89e3\u6790\u5ea6\uff0c\u5f9e\u539f\u672c\u4e00\u822c\u986f\u5fae\u93e1\u7684\u89e3\u6790\u5ea6\u5927\u5927\u7684\u63d0\u5347\u3002<\/p>\n

2006\u5e74\u767c\u5c55\u7684PALM\u548cSTORM\u5169\u7a2e\u986f\u5fae\u8853\u975e\u5e38\u985e\u4f3c\uff0c\u57fa\u672c\u4e0a\u7d50\u5408\u4e86\u4e09\u500b\u65b9\u6cd5\uff1a<\/p>\n

\uff08\u4e00\uff09\u5982\u679c\u6240\u6709\u6771\u897f\u4e00\u8d77\u4eae\uff0c\u5168\u90e8\u9ecf\u5728\u4e00\u8d77\u770b\u4e0d\u6e05\u695a\uff0c\u90a3\u5c31\u627e\u4e00\u4e9b\u7279\u6b8a\u7684\u9583\u52d5\u87a2\u5149\u5206\u5b50\uff0c\u6216\u52a0\u5165\u7279\u6b8a\u7684\u5316\u5b78\u6eb6\u6db2\u4f7f\u87a2\u5149\u5206\u5b50\u9583\u52d5\uff0c\u63a7\u5236\u5b83\u9583\u52d5\u7684\u983b\u7387\uff0c\u8b93\u5b83\u5927\u90e8\u5206\u7684\u6642\u5019\u662f\u6697\u7684\u3002\u4e5f\u5c31\u662f\u8aaa\uff0c\u672c\u4f86\u5169\u500b\u5f88\u8cbc\u8fd1\u7684\u5206\u5b50\uff0c\u5982\u679c\u4e00\u8d77\u4eae\u5206\u8fa8\u4e0d\u51fa\u4f86\uff0c\u5c31\u63a7\u5236\u5b83\u4e00\u500b\u5148\u4eae\uff0c\u4e00\u500b\u5f8c\u4eae\u3002
\n\uff08\u4e8c\uff09\u7b2c\u4e8c\u500b\u65b9\u6cd5\u5c31\u662f\uff0c\u6bcf\u4e00\u500b\u4eae\u9ede\u96d6\u7136\u5b83\u7684\u5927\u5c0f\u9084\u662f\u5927\u7d04250\u5948\u7c73\uff0c\u53ef\u662f\u7531\u65bc\u53ea\u662f\u5f9e\u4e00\u500b\u5149\u6e90\u51fa\u4f86\u7684\uff0c\u57fa\u672c\u4e0a\u4ed6\u7684\u5206\u4f48\u61c9\u8a72\u662f\u4e2d\u9593\u4eae\u65c1\u908a\u6697\uff0c\u5982\u679c\u5149\u5b50\u7684\u91cf\u5920\uff0c\u5c31\u53ef\u4ee5\u7528\u4e00\u500b\u6578\u5b78\u5e38\u614b\u5206\u4f48\u7684\u5047\u8a2d\uff0c\u731c\u6e2c\u5b83\u4e2d\u5fc3\u7684\u4f4d\u7f6e\uff0c\u6e96\u78ba\u6c7a\u5b9a\u5149\u6e90\u662f\u5f9e\u54ea\u4e00\u9ede\u4f86\u7684\u3002
\n\uff08\u4e09\uff09\u7b2c\u4e09\uff0c\u518d\u628a\u4e0a\u5343\u5f35\u4e0a\u842c\u5f35\u55ae\u4e00\u9583\u52d5\u7684\u7167\u7247\uff0c\u5168\u90e8\u758a\u52a0\u8d77\u4f86\uff0c\u6bcf\u5f35\u7167\u7247\u90fd\u662f\u8d85\u9ad8\u89e3\u6790\u5ea6\uff0c\u758a\u52a0\u8d77\u4f86\u53ef\u4ee5\u770b\u5230\u8d85\u9ad8\u89e3\u6790\u5ea6\u7684\u5168\u666f\uff0cPALM\u4ee5\u76ee\u524d\u7684\u87a2\u5149\u5206\u5b50\u4eae\u5ea6\u4f86\u8aaa\u53ef\u4ee5\u9054\u523020\u5948\u7c73\u7684\u89e3\u6790\u5ea6\u3002STED\u548cPALM\u5169\u7a2e\u8d85\u9ad8\u89e3\u6790\u5ea6\u90fd\u662f\u975e\u5e38\u5de7\u5999\u7684\u65b9\u6cd5\uff0c\u7a81\u7834\u4e86\u767e\u5e74\u4f86\u5149\u5b78\u89e3\u6790\u7684\u6975\u9650\uff0c\u8b93\u751f\u7269\u5b78\u5bb6\u9032\u4e00\u5927\u6b65\u7684\u770b\u5230\u539f\u672c\u4e0d\u80fd\u770b\u5230\u7684\u751f\u7269\u5206\u5b50\u7d50\u69cb\u3002\u7576\u7136\u9084\u6709\u5176\u4ed6\u8a31\u591a\u4e0d\u540c\u7684\u8d85\u9ad8\u89e3\u6790\u5ea6\u7684\u65b9\u6cd5\uff0c\u5305\u62ecSIM\u3001PAINT\u3001 \u53ca\u64f4\u5c55\u986f\u5fae\u8853\u7b49\uff0c\u5728\u6b64\u5148\u4e0d\u4e00\u4e00\u4ecb\u7d39\u3002<\/p>\n

\u6211\u5011\u7684\u5be6\u9a57\u5ba4\u5f9e2008\u5e74\u958b\u59cb\u8d85\u9ad8\u89e3\u6790\u5ea6\u986f\u5fae\u8853\u7684\u5be6\u9a57\uff0c\u4e3b\u8981\u7684\u751f\u7269\u984c\u76ee\u5c31\u662f\u4e86\u89e3\u4e3b\u7e96\u6bdb\u5e95\u90e8\u4ee5\u53ca\u4e2d\u5fc3\u7c92\u9802\u90e8\uff0c\u5305\u62ec\u904e\u6e21\u5340\u3001\u9060\u7aef\u9644\u5c6c\u7269\u3001\u53ca\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u7684\u7d50\u69cb\uff0c\u767c\u8868\u4e863\u7bc7\u6587\u7ae0\u5206\u5225\u65bc Scientific Reports\u3001Nature Communications\u3001\u53ca eLife\u3002\u5728\u904e\u6e21\u5340\u7684\u90e8\u5206\uff0c\u6211\u5011\u770b\u6e05\u695a\u4e86\u54ea\u4e9b\u86cb\u767d\u8cea\u6bd4\u8f03\u9760\u8fd1\u4e3b\u7e96\u6bdb\u4e2d\u9593\u985e\u4f3c\u9435\u8def\u8ecc\u9053\u7684\u90e8\u5206\uff0c\u54ea\u4e9b\u86cb\u767d\u8cea\u6bd4\u8f03\u9760\u8fd1\u5916\u9762\u7e96\u6bdb\u819c\u7684\u90e8\u5206\uff0c\u53ef\u4ee5\u4e86\u89e3\u54ea\u4e9b\u86cb\u767d\u8cea\u662f\u6bd4\u8f03\u6709\u53ef\u80fd\u64d4\u4efb\u819c\u86cb\u767d\u7684\u63a7\u7ba1\uff0c\u54ea\u4e9b\u86cb\u767d\u8cea\u6bd4\u8f03\u662f\u8ca0\u8cac\u628a\u904e\u6e21\u5340\u67b6\u5728\u8ecc\u9053\u4e0a\uff0c\u4f7f\u6574\u500b\u4e3b\u7e96\u6bdb\u7814\u7a76\u7684\u9818\u57df\u80fd\u5920\u66f4\u9032\u4e00\u6b65\u7684\u5728\u7d50\u69cb\u4e0a\u4e86\u89e3\u5404\u86cb\u767d\u8cea\u7684\u529f\u80fd\uff0c\u800c\u4e0d\u662f\u4e4b\u524d\u5341\u5e7e\u5e74\u4f86\u86cb\u767d\u8cea\u90fd\u4e0d\u6e05\u695a\u5728\u54ea\u88e1\uff0c\u6c92\u6709\u7d50\u69cb\u8a8d\u77e5\uff0c\u7d14\u7cb9\u57fa\u56e0\u65b9\u6cd5\u7814\u7a76\u731c\u6e2c\u4ed6\u5011\u7684\u4f4d\u7f6e\u3002\u5728\u9060\u7aef\u9644\u5c6c\u7269\u65b9\u9762\uff0c\u6211\u5011\u628a9\u500b\u5c0d\u7a31\u7684\u6247\u8449\u72c0\u7684\u7d50\u69cb\u4e0a\u86cb\u767d\u8cea\u7684\u76f8\u5c0d\u4f4d\u7f6e\uff0c\u5f04\u6e05\u695a\u54ea\u4e00\u500b\u662f\u6bd4\u8f03\u9760\u8fd1\u6700\u5c16\u7aef\u63a5\u89f8\u7e96\u6bdb\u819c\u7684\u90e8\u5206\uff0c\u54ea\u4e00\u500b\u662f\u5728\u6700\u6839\u90e8\uff0c\u8b93\u6240\u6709\u7684\u6771\u897f\u5f80\u4e0a\u9577\u7684\u57fa\u5e95\uff0c\u54ea\u4e00\u500b\u662f\u5305\u8986\u5728\u6247\u8449\u5916\u9762\uff0c\u8b93\u9019\u4e9b\u9060\u7aef\u9644\u5c6c\u7269\u5404\u81ea\u7684\u86cb\u767d\u4f4d\u7f6e\u8207\u4e4b\u524d\u57fa\u56e0\u65b9\u6cd5\u505a\u51fa\u4f86\u7684\u5be6\u9a57\u505a\u9a57\u8b49\u3002<\/p>\n

\u9664\u6b64\u4e4b\u5916\uff0c\u6211\u5011\u66f4\u4e86\u89e3\u5176\u4e2d\u67d0\u4e00\u86cb\u767d\u80fd\u5920\u8ca0\u8cac\u7e96\u6bdb\u819c\u86cb\u767d\u9032\u51fa\u7684\u63a7\u5236\uff0c\u53e6\u5916\u57289\u500b\u5c0d\u7a31\u7684\u6247\u8449\u72c0\u7d50\u69cb\u4e4b\u9593\u767c\u73fe\u5176\u5be6\u662f\u6709\u7269\u8cea\u5b58\u5728\u7684\u3002\u4ee5\u524d\u8a8d\u70ba\u9019\u500b\u90e8\u5206\u6c92\u6709\u6771\u897f\uff0c\u4f46\u662f\u6211\u5011\u767c\u73fe\u9019\u500b\u7d50\u69cb\u662f\u6709\u8abf\u63a7\u7684\u529f\u80fd\u7684\uff0c\u6211\u5011\u7a31\u4e4b\u70ba\u9060\u7aef\u9644\u5c6c\u7269matrix\u3002\u9019\u662f\u4e00\u500b\u65b0\u767c\u73fe\uff0c\u662f\u6c92\u6709\u8d85\u9ad8\u89e3\u6790\u5ea6\u986f\u5fae\u8853\u5c31\u6c92\u6709\u8fa6\u6cd5\u8fa6\u5230\u7684\u3002\u5728\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u65b9\u9762\uff0c\u6211\u5011\u767c\u73fe\u5b83\u5011\u5176\u5be6\u662f\u6bd4\u8f03\u4e0d\u898f\u5247\u7684\u5e7e\u500b\u6247\u8449\uff0c\u9019\u4e9b\u6247\u8449\u6c92\u6709\u50cf\u9060\u7aef\u9644\u5c6c\u7269\u7684\u7d50\u69cb\u9019\u9ebc\u7a69\u5b9a\uff0c\u53ef\u4ee5\u8aaa\u6bd4\u8f03\u8edf\u4e00\u9ede\uff0c\u800c\u4e14\u4e5f\u4e0d\u4e00\u5b9a9\u500b\u90fd\u6703\u9577\u51fa\u4f86\uff0c\u6709\u6642\u6703\u5c11\u4e00\u4e9b\uff0c\u9577\u51fa\u4f86\u7684\u89d2\u5ea6\u4e5f\u4e0d\u898b\u5f97\u90fd\u4e00\u6a23\u3002\u6211\u5011\u4e5f\u4e00\u4e00\u89e3\u6790\u5728\u9019\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u4e0a\uff0c\u5404\u86cb\u767d\u8cea\u7684\u76f8\u5c0d\u4f4d\u7f6e\u3002\u66f4\u6709\u8da3\u7684\u662f\uff0c\u6211\u5011\u767c\u73fe\u96d6\u7136\u9060\u7aef\u9644\u5c6c\u7269\u548c\u6b21\u7aef\u9644\u5c6c\u7269\u8ca0\u8cac\u7684\u529f\u80fd\u4e0d\u540c\uff0c\u4e00\u500b\u7ba1\u7e96\u6bdb\u7684\u6210\u9577\u3001\u53e6\u5916\u4e00\u500b\u7ba1\u5fae\u7ba1\u7db2\u5f80\u5916\u7684\u5ef6\u4f38\u3002\u4f46\u662f\u6211\u5011\u767c\u73fe\u5b83\u5011\u7684\u7d50\u69cb\u662f\u606f\u606f\u76f8\u95dc\u7684\uff0c\u628a\u5176\u4e2d\u4e00\u500b\u7d50\u69cb\u5f04\u6389\uff0c\u53e6\u5916\u4e00\u500b\u7d50\u69cb\u5c31\u6703\u6709\u986f\u8457\u7684\u8b8a\u5316\u6216\u662f\u4f4d\u7f6e\u7684\u6539\u8b8a\u3002\u6211\u5011\u4e5f\u6e05\u695a\u7684\u770b\u5230\u5c07\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u53bb\u6389\uff0c\u5ef6\u4f38\u51fa\u53bb\u7684\u5fae\u7ba1\u5728\u9019\u500b\u9644\u8fd1\u5c31\u6703\u6e1b\u5c11\uff0c\u76f4\u63a5\u5730\u8b49\u660e\u4e86\u4ed6\u5011\u652f\u6490\u5fae\u7ba1\u7684\u529f\u80fd\u3002<\/p>\n

\u9019\u4e00\u7cfb\u5217\u4e09\u7bc7\u8d85\u9ad8\u89e3\u6790\u5ea6\u986f\u5fae\u8853\u7684\u7814\u7a76\uff0c\u4ed4\u7d30\u7684\u5206\u6790\u7684\u9019\u4e00\u500b\u90e8\u520620\u7a2e\u4ee5\u4e0a\u86cb\u767d\u8cea\u7684\u76f8\u5c0d\u4f4d\u7f6e\uff0c\u8b93\u6211\u5011\u5c0d\u4e3b\u7e96\u6bdb\u548c\u4e2d\u5fc3\u7c92\u6709\u66f4\u5168\u9762\u6027\u4ee5\u7d50\u69cb\u70ba\u57fa\u790e\u7684\u4e86\u89e3\uff0c\u4e5f\u8b93\u751f\u7269\u5b78\u5bb6\u80fd\u5920\u4e0d\u7528\u518d\u778e\u5b50\u6478\u8c61\u822c\u5730\u53ea\u7528\u57fa\u56e0\u65b9\u6cd5\u731c\u6e2c\u5404\u86cb\u767d\u8cea\u7684\u4f4d\u7f6e\u3002\u9084\u6709\u975e\u5e38\u591a\u7684\u751f\u7269\u7d50\u69cb\u7b49\u8457\u7528\u8d85\u9ad8\u89e3\u6790\u5ea6\u986f\u5fae\u8853\u89c0\u5bdf\uff0c\u800c\u5176\u5be6\u6709\u53ef\u80fd\u5728\u4e0d\u4e45\u7684\u5c07\u4f86\uff0c\u6211\u5011\u53ef\u4ee5\u9054\u5230\u66f4\u9ad8\u7684\u89e3\u6790\u5ea6\uff0c\u5efa\u9020\u7d50\u69cb\u751f\u7269\u5b78\u53ca\u7d30\u80de\u751f\u7269\u5b78\u4e4b\u9593\u7684\u6a4b\u6a11\u3002<\/p>\n

\"\"

\u5716\u4e8c \u4ee5STED\u8d85\u9ad8\u89e3\u6790\u5ea6\u5efa\u69cb\u7684\u4e3b\u7e96\u6bdb\u904e\u6e21\u5340\u6a21\u578b\u53ca\u4ee5STORM\u8d85\u9ad8\u89e3\u6790\u5ea6\u5efa\u69cb\u7684\u4e2d\u5fc3\u7c92\u9060\u7aef\u9644\u5c6c\u7269\u53ca\u6b21\u9060\u7aef\u9644\u5c6c\u7269\u6a21\u578b<\/p><\/div>\n

\u53c3\u8003\u6587\u737b
\n1. Chong, W.M., Wang, W.-J., Lo, C.-H., Chiu, T.-Y., Chang, T.-J., Liu, Y.-P., Tanos, B., Mazo, G., Tsou, M.-F.B., Jane, W.-N., Yang, TT., Liao, J.-C. (2020) Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages. eLife, 9, e53580.<\/p>\n

2. Yang, TT., Chong, W.M., Wang, W.-J., Mazo G., Tanos, B., Chen, Z., Tran T.M.N., Chen, Y.-D., Weng, R.R., Huang, C.-E., Jane, W.-N. Tsou, M.-F.B., Liao, J.-C. (2018) Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components. Nature Communications, 9, 2023.<\/p>\n

3. Yang, TT., Su, J., Wang, W.-J., Craige, B., Witman, G.B., Tsou, M.-F.B., Liao, J.-C. (2015) Superresolution pattern recognition reveals architectural map of the ciliary transition zone, Scientific Reports, 5, 14096.<\/p>\n","protected":false},"excerpt":{"rendered":"

\u4f5c\u8005\uff1a\u5ed6\u4ef2\u9e92\u7814\u7a76\u54e1\uff08\u672c\u9662\u539f\u5b50\u8207\u5206\u5b50\u79d1\u5b78\u7814\u7a76\u6240\uff09 \u52d5\u7269\u7d30\u80de\u5206\u88c2\u7684\u6642\u5019\uff0c\u6709\u4e00\u7d44\u5c0f\u7684\u7d50\u69cb\u4e5f\u6703\u8ddf\u8457\u7d30\u80de\u4e00\u8d77\u8907\u88fd\uff0c\u518d\u5206\u5230 […]<\/p>\n","protected":false},"author":1,"featured_media":21750,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[991,983],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/21747"}],"collection":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/comments?post=21747"}],"version-history":[{"count":1,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/21747\/revisions"}],"predecessor-version":[{"id":21782,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/21747\/revisions\/21782"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media\/21750"}],"wp:attachment":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media?parent=21747"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/categories?post=21747"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/tags?post=21747"}],"curies":[{"name":"wp","href":"https:\/api.w.org\/{rel}","templated":true}]}}