{"id":20251,"date":"2019-10-31T00:05:03","date_gmt":"2019-10-30T16:05:03","guid":{"rendered":"https:\/newsletter.sinica.edu.tw\/?p=20251"},"modified":"2021-08-04T09:16:09","modified_gmt":"2021-08-04T01:16:09","slug":"20191031p01","status":"publish","type":"post","link":"https:\/newsletter.sinica.edu.tw\/20251\/","title":{"rendered":"\u3010\u5c08\u6b04\u3011\u6709\u6a5f\u5316\u5b78\u8207\u751f\u547d\u79d1\u5b78\u7f8e\u9e97\u7684\u7d50\u5408\uff1a\u751f\u7269\u6b63\u4ea4\u6027\u5316\u5b78"},"content":{"rendered":"<p>\u9577\u671f\u4ee5\u4f86\uff0c\u751f\u7269\u5b78\u5bb6\u6e34\u671b\u85c9\u7531\u767c\u5c55\u5404\u7a2e\u7814\u7a76\u7b56\u7565\u671f\u5f85\u4e86\u89e3\u751f\u7269\u6210\u9577\u4ee3\u8b1d\u6a5f\u5236\uff0c\u5f9e\u4e2d\u627e\u51fa\u751f\u547d\u7a0b\u5e8f\u7684\u5967\u5999\uff0c\u4ee5\u53ca\u91dd\u5c0d\u75be\u75c5\u627e\u51fa\u56e0\u61c9\u6cbb\u7642\u4e4b\u9053\u3002\u5341\u4e03\u4e16\u7d00\u5b89\u6771\u5c3c\u00b7\u8303\u00b7\u96f7\u6587\u970d\u514b(Antonie van Leeuwenhoek)\u767c\u660e\u986f\u5fae\u93e1\u4e4b\u5f8c\uff0c\u79d1\u5b78\u5bb6\u5c0d\u65bc\u751f\u7269\u5967\u79d8\u7684\u63a2\u7d22\u4fbf\u4e0d\u518d\u662f\u778e\u5b50\u6478\u8c61\u5f0f\u5728\u9ed1\u6697\u4e2d\u6478\u7d22\uff0c\u4e00\u500b\u9109\u6c11\u6a19\u699c\u300c\u6709\u5716\u6709\u771f\u76f8\u300d\u7684\u7814\u7a76\u8996\u91ce\u88ab\u958b\u555f\uff0c\u4e00\u7cfb\u5217\u5fae\u5c0f\u7684\u751f\u7269\u7d30\u80de\u3001\u7d30\u83cc\u53ca\u75c5\u6bd2\uff0c\u751a\u81f3\u65bc\u4e00\u4e9b\u904e\u53bb\u672a\u77e5\u7684\u751f\u547d\u7d30\u5fae\u500b\u9ad4\u5728\u986f\u5fae\u93e1\u4e0b\u7121\u6240\u9041\u5f62\uff0c\u4e00\u4e00\u63ed\u958b\u5176\u795e\u79d8\u7684\u9762\u7d17\u3002\u7136\u800c\uff0c\u79d1\u5b78\u5bb6\u4e26\u4e0d\u7518\u65bc\u73fe\u72c0\uff0c\u5e0c\u671b\u9032\u4e00\u6b65\u5730\u4e86\u89e3\u751f\u547d\u7d30\u80de\u5167\u5404\u7a2e\u751f\u7269\u5206\u5b50\u9593\u7684\u4ea4\u4e92\u4f5c\u7528\u53ca\u5176\u8a0a\u606f\u529f\u80fd\u50b3\u905e\uff0c\u5982\u4f55\u7dad\u6301\u7d30\u80de\u7684\u7279\u6027\u8207\u500b\u9ad4\u6027\uff0c\u4f8b\u5982\u751f\u547d\u7684\u8d77\u6e90\u4f86\u81ea\u65bc\u7d30\u80de\u5167\u7684\u57fa\u56e0\u907a\u50b3\u8a0a\u606f\u5206\u5b50:\u53bb\u6c27\u6838\u7cd6\u6838\u9178(DNA)\u8207\u6838\u7cd6\u6838\u9178(RNA)\uff0c\u6bcf\u7576\u7d30\u80de\u5206\u88c2\u6642\uff0cDNA\u53ef\u4ee5\u81ea\u884c\u8907\u88fd\uff08replication\uff09\uff0c\u56e0\u800c\u78ba\u4fdd\u6bcf\u4e00\u4ee3\u7684\u7d30\u80de\u90fd\u5e36\u6709\u540c\u6a23\u57fa\u56e0\u907a\u50b3\u7279\u6027\u3002\u7576\u7d30\u80de\u9700\u8981\u8868\u73fe\u6240\u9700\u86cb\u767d\u8cea\u6642\uff0c\u6703\u5c07DNA\u7684\u8a0a\u606f\u8f49\u9304\u5230RNA\u4e0a\uff08transcription\uff09\uff0c\u518d\u7531RNA\u8f49\u8b6f\u751f\u6210\u86cb\u767d\u8cea\uff08translation\uff09\uff0c\u800c\u8868\u9054\u51fa\u7684\u86cb\u767d\u8cea\u5247\u6703\u57f7\u884c\u7d30\u80de\u6240\u9700\u8981\u7684\u529f\u80fd\uff0c\u9019\u4e5f\u662f\u5206\u5b50\u751f\u7269\u5b78\u7684\u4e2d\u5fc3\u6cd5\u5247\u3002\u7136\u800c\uff0c\u7d30\u80de\u5167\u7684\u5b58\u5728\u8457\u591a\u6a23\u5316\u4e14\u5177\u5404\u7a2e\u4e0d\u540c\u529f\u80fd\u6027\u86cb\u767d\u8cea\u4ee5\u7dad\u6301\u7d30\u80de\u751f\u9577\u7684\u904b\u4f5c\uff0c\u5982\u4f55\u4e86\u89e3\u55ae\u4e00\u86cb\u767d\u8cea\u5728\u7d30\u80de\u88e1\u9762\u6240\u626e\u6f14\u7684\u89d2\u8272\u53ca\u5176\u904b\u4f5c\u4e0a\u91cd\u8981\u6027\u5247\u662f\u79d1\u5b78\u5bb6\u6240\u9762\u81e8\u7684\u7814\u7a76\u8ab2\u984c\u3002<\/p>\n<p>\u7da0\u8272\u87a2\u5149\u86cb\u767d(\u7c21\u7a31GFP\uff0cGreen Fluorescent Protein)\u7684\u7814\u7a76\uff0c\u70ba\u4e0a\u8ff0\u7684\u7814\u7a76\u9818\u57df\u9ede\u4e86\u4e00\u76de\u660e\u71c8\uff0c\u540c\u6642\u9707\u64bc\u9019\u500b\u7814\u7a76\u9818\u57df\u4f5c\u51fa\u4e86\u8de8\u6642\u4ee3\u7684\u9769\u547d\u6027\u5f71\u97ff\uff0c\u5176\u5f71\u97ff\u5c64\u9762\u4e4b\u5ee3\uff0c\u5305\u542b\u7d30\u80de\u5206\u5b50\u751f\u7269\u5b78\u3001\u85e5\u7269\u958b\u767c\u53ca\u5176\u751f\u7269\u611f\u6e2c\u6280\u8853\u7b49\u9818\u57df\u3002\u56e0\u70ba\u63a5\u4e0b\u4f86\u7684\u7814\u7a76\u8005\u53ea\u9700\u5c07GFP\u57fa\u56e0\u8f49\u6b96\u81f3\u6240\u9700\u89c0\u5bdf\u7684\u86cb\u767d\u8cea\u4e0a\u5373\u53ef\u5229\u7528GFP\u767c\u5149\u5718\u7279\u6027\uff0c\u4ee5\u87a2\u5149\u986f\u5fae\u93e1\u8089\u773c\u89c0\u5bdf\u7279\u5b9a\u86cb\u767d\u8cea\u5728\u7d30\u80de\u5167\u7684\u4f4d\u7f6e\u3001\u52d5\u614b\u53ca\u5176\u4f5c\u7528\uff0c\u9032\u800c\u4e86\u89e3\u5982\u4f55\u5f71\u97ff\u7d30\u80de\u6210\u9577\u904b\u4f5c\uff0c\u5982\u540c\u70ba\u86cb\u767d\u8cea\u751f\u7269\u5206\u5b50\u7c21\u55ae\u6234\u4e0a\u667a\u6167\u7a7f\u6234\u88dd\u7f6e\uff0c\u4fbf\u53ef\u5bb9\u6613\u6e05\u695a\u4e86\u89e3\u8a72\u86cb\u767d\u8cea\u5728\u7d30\u80de\u5167\u7684\u4e00\u8209\u4e00\u52d5\u3002\u6b63\u75762008\u5e74\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u9812\u767c\u7d66\u4e0b\u6751\u8129(Osamu Shimomura)\u3001\u99ac\u4e01\u55ac\u975e(Martin Chalfie)\u8207\u9322\u6c38\u5065(Roger Y. Tsien)\u4e09\u4f4d\u79d1\u5b78\u5bb6\u4ee5\u6a19\u699c\u5c0dGFP\u91cd\u5927\u7814\u7a76\u8ca2\u737b\u7684\u540c\u6642\uff0c\u79d1\u5b78\u5bb6\u4e5f\u958b\u59cb\u601d\u8003\u53e6\u4e00\u500b\u7814\u7a76\u8ab2\u984c\uff0c\u7d30\u80de\u5167\u7684\u91cd\u8981\u751f\u7269\u5206\u5b50\u4e0d\u50c5\u6b62\u65bc\u86cb\u767d\u8cea\uff0c\u907a\u50b3\u50b3\u905e\u5206\u5b50DNA\u8207RNA\u3001\u8102\u8cea\u548c\u86cb\u767d\u8cea\u5f8c\u4fee\u98fe\u7684\u91a3\u985e\u5206\u5b50\u540c\u6a23\u8868\u73fe\u4e86\u5f71\u97ff\u7d30\u80de\u7684\u904b\u4f5c\u53ca\u5176\u4e0d\u53ef\u5ffd\u8996\u7684\u751f\u7406\u6d3b\u6027\uff0c\u5982\u4f55\u767c\u5c55\u5f4c\u88dcGFP\u57fa\u56e0\u8f49\u6b96\u6280\u8853\u50c5\u80fd\u87a2\u5149\u6a19\u5b9a\u86cb\u767d\u8cea\u7684\u7814\u7a76\u7b56\u7565\uff0c\u958b\u767c\u91dd\u5c0d\u5176\u4ed6\u7279\u5b9a\u751f\u7269\u5206\u5b50\u4fee\u98fe\u6a19\u5b9a\u7684\u79d1\u5b78\u65b9\u6cd5\uff0c\u4ee5\u62d3\u5c55\u6211\u5011\u5c0d\u9019\u4e9b\u751f\u7269\u5206\u5b50\u7684\u8a8d\u77e5\u53ca\u6df1\u5c64\u4e86\u89e3\u3002<\/p>\n<p>\u751f\u7269\u6b63\u4ea4\u6027\u5316\u5b78(bioorthogonal chemistry)\u662f\u6e90\u81ea\u65bc\u7576\u6642\u4efb\u6559\u65bc\u52a0\u5dde\u5927\u5b78\u67cf\u514b\u840a\u5206\u6821\u5316\u5b78\u7cfb\u6559\u6388\u5361\u7f85\u7433\u00b7\u8c9d\u723e\u6258\u897f(Carolyn R. Bertozzi)\u57282003\u5e74\u6240\u63d0\u51fa\u7684\u6982\u5ff5[1]\uff0c\u6307\u7684\u662f\u53ef\u5728\u6d3b\u9ad4\u7d30\u80de\u3001\u751f\u7269\u7d44\u7e54\u4e2d\u9032\u884c\u9ad8\u6548\u80fd\u7279\u7570\u7684\u5916\u6e90\u6027\u5316\u5b78\u53cd\u61c9\uff0c\u4e26\u4e14\u8207\u7d30\u80de\u4ee3\u8b1d\u904e\u7a0b\u4e2d\u8907\u96dc\u751f\u5316\u53cd\u61c9\u4e92\u4e0d\u5e72\u64fe\uff0c\u900f\u904e\u6b64\u4e00\u5316\u5b78\u7b56\u7565\uff0c\u4e0d\u50c5\u53ef\u87a2\u5149\u6a19\u5b9a\u7279\u5b9a\u751f\u7269\u5206\u5b50\uff0c\u540c\u6642\u53ef\u85c9\u7531\u7d30\u5fae\u7684\u5316\u5b78\u4fee\u98fe\u7814\u7a76\u751f\u7269\u5927\u5206\u5b50\uff0c\u4ee5\u4eba\u70ba\u624b\u6bb5\u5e72\u64fe\u6216\u8abf\u63a7\u751f\u547d\u7cfb\u7d71\uff0c\u5f9e\u4e2d\u95e1\u8ff0\u8907\u96dc\u751f\u7269\u9ad4\u7cfb\u904b\u4f5c\u7684\u751f\u7269\u4ee3\u8b1d\u6a5f\u5236\u3002\u6709\u6a5f\u5316\u5b78\u5728\u7814\u7a76\u751f\u547d\u79d1\u5b78\u9818\u57df\u65b9\u9762\u76f8\u8f03\u65bc\u5176\u4ed6\u5316\u5b78\u5206\u652f\u5b78\u79d1\u64c1\u6709\u5f97\u5929\u7368\u539a\u7684\u512a\u52e2\uff0c\u5316\u5b78\u5bb6\u900f\u904e\u5408\u7406\u7684\u8a2d\u8a08\u5408\u6210\u4e0d\u540c\u529f\u80fd\u6027\u7684\u6709\u6a5f\u5c0f\u5206\u5b50\u4f5c\u70ba\u5316\u5b78\u63a2\u91dd\uff0c\u53ef\u4ee5\u6709\u6548\u5730\u63a2\u7d22\u8907\u96dc\u7684\u751f\u547d\u9ad4\u7cfb\uff0c\u56e0\u6b64\u900f\u904e\u5316\u5b78\u5de5\u5177\u548c\u7814\u7a76\u7b56\u7565\u8a8d\u8b58\u8907\u96dc\u751f\u547d\u9ad4\u7cfb\u7684\u5316\u5b78\u751f\u7269\u5b78\u9818\u57df\u8fd1\u5e74\u4f86\u65e5\u76ca\u84ec\u52c3\u767c\u5c55\u3002\u5361\u7f85\u7433\u00b7\u8c9d\u723e\u6258\u897f\u6559\u6388\u8a8d\u70ba\uff0c\u7d30\u80de\u5206\u5b50\u4ee3\u8b1d\u6a5f\u5236\u56fa\u7136\u8907\u96dc\uff0c\u4f46\u662f\u4f9d\u7136\u4f9d\u5faa\u6709\u898f\u5f8b\u7684\u5316\u5b78\u53cd\u61c9\u7d44\u5408\u800c\u6210\uff0c\u8207\u4e00\u822c\u55ae\u7d14\u5316\u5b78\u9375\u751f\u6210\u6216\u65b7\u88c2\u7684\u6709\u6a5f\u5316\u5b78\u53cd\u61c9\u6709\u6240\u4e0d\u540c\u4e4b\u8655\uff0c\u5728\u65bc\u751f\u547d\u9ad4\u7cfb\u4e2d\u5145\u65a5\u8457\u8a31\u591a\u9175\u7d20\u76f8\u4e92\u5354\u540c\u6216\u62ee\u6297\u4f5c\u7528\u7684\u8d85\u5206\u5b50\u5316\u5b78\u7d44\u88dd\u548c\u5206\u89e3\u53cd\u61c9\u3002\u70ba\u4e86\u6df1\u5165\u7814\u7a76\u548c\u8a8d\u8b58\u9019\u4e9b\u8907\u96dc\u7684\u751f\u547d\u904e\u7a0b\uff0c\u767c\u5c55\u300c\u53ef\u8996\u5316\u300d\u7814\u7a76\u5de5\u5177\u548c\u7b56\u7565\u65b9\u6cd5\uff0c\u5c07\u6709\u52a9\u65bc\u7814\u7a76\u8005\u63ed\u958b\u9019\u4e9b\u4ee3\u8b1d\u904e\u7a0b\u7684\u795e\u79d8\u9762\u7d17\uff0c\u5f9e\u800c\u77ad\u89e3\u751f\u7269\u5206\u5b50\u7684\u91cd\u8981\u6027\u53ca\u529f\u80fd\u6027\u3002<\/p>\n<p>\u8fd1\u5e74\u4f86\uff0c\u6709\u6a5f\u5316\u5b78\u5bb6\u958b\u767c\u4e86\u4e00\u7a2e\u540d\u70ba\u300c\u9ede\u64ca\u5316\u5b78\u300d(click chemistry)\u7684\u5f37\u5927\u5de5\u5177\uff0c\u9867\u540d\u601d\u7fa9\uff0c\u9019\u7a2e\u5316\u5b78\u53cd\u61c9\u7279\u6027\u5728\u65bc\u53cd\u61c9\u6d3b\u6027\u6975\u4f73\uff0c\u5982\u540c\u4e00\u6309\u6ed1\u9f20\u7684\u77ac\u9593\uff0c\u53cd\u61c9\u4fbf\u8fc5\u901f\u5b8c\u6210\uff0c\u56e0\u6b64\u300c\u9ede\u64ca\u5316\u5b78\u300d\u4fbf\u6210\u70ba\u5316\u5b78\u751f\u7269\u5b78\u5bb6\u7814\u7a76\u751f\u7269\u9ad4\u7cfb\u5206\u5b50\u7684\u4e00\u500b\u6838\u5fc3\u5316\u5b78\u5de5\u5177\uff0c\u7528\u65bc\u5316\u5b78\u6a19\u8a18\u548c\u8ffd\u8e64\u8907\u96dc\u751f\u547d\u9ad4\u7cfb\u4e2d\u751f\u7269\u5de8\u5927\u5206\u5b50\u6216\u529f\u80fd\u6027\u5c0f\u5206\u5b50\u3002\u300c\u9ede\u64ca\u5316\u5b78\u300d\u9996\u63a8\u7576\u6642\u4efb\u8077\u53f2\u666e\u5229\u514b\u65af\u7814\u7a76\u6240\uff08The Scripps Research Institute\uff09\u8c9d\u745e\u00b7\u590f\u666e\u5229\u65af\uff08K. Barry Sharpless\uff09\u6559\u6388\u57282001\u5e74\u767c\u5c55\u51fa\u4e00\u7a2e\u5229\u7528\u4e00\u50f9\u9285\u96e2\u5b50\u50ac\u5316\u758a\u6c2e\u5316\u7269\uff08azide\uff09\u548c\u7aef\u7094\u985e\uff08alkyne\uff09\u4f5c\u7528\u5f62\u6210\u5171\u50f9\u9375\uff0c\u4e26\u751f\u6210\u96dc\u74b0\u4e09\u5511\u985e\u5316\u5408\u7269\u7684\u5316\u5b78\u53cd\u61c9\uff0c\u7c21\u7a31CuAAC [Copper(I)-catalyzed Azide-Alkyne Cycloaddition][2]\uff0c\u6b64\u985e\u578b\u5316\u5b78\u7279\u6027\u5728\u65bc\uff0c\u80fd\u5728\u5e38\u6eab\u689d\u4ef6\u4e0b\u7684\u6c34\u4e2d\u9032\u884c\u53cd\u61c9\uff0c\u800c\u4e14\u4e0d\u53d7\u53cd\u61c9\u74b0\u5883pH\u503c\u5f71\u97ff\u53cd\u61c9\u6d3b\u6027\uff0c\u751a\u81f3\u512a\u5316\u5f8c\u7684\u53cd\u61c9\u689d\u4ef6\u80fd\u5728\u6d3b\u7d30\u80de\u4e2d\u9032\u884c\u9ad8\u6548\u80fd\u548c\u9ad8\u63a7\u5236\u6027\u53cd\u61c9\u9032\u884c\u5206\u5b50\u6a19\u5b9a\uff0c\u885d\u64ca\u4e00\u822c\u4eba\u8a8d\u70ba\u6709\u6a5f\u5316\u5b78\u53cd\u61c9\u5fc5\u9808\u5b58\u5728\u65bc\u6709\u6a5f\u6eb6\u5291\u4e26\u9700\u52a0\u71b1\u4e0b\u65b9\u53ef\u9032\u884c\u53cd\u61c9\u7684\u8a8d\u77e5\uff0c\u7576\u6642\u5728\u5316\u5b78\u5408\u6210\u53ca\u5316\u5b78\u751f\u7269\u5b78\u9818\u57df\u5f15\u8d77\u4e86\u6975\u5927\u7684\u6ce8\u76ee\u3002\u4ee5CuAAC\u70ba\u4ee3\u8868\u7684\u9ede\u64ca\u5316\u5b78\u6982\u5ff5\u4e0d\u53ea\u5c0d\u5316\u5b78\u5408\u6210\u9818\u57df\u6709\u5f88\u5927\u7684\u8ca2\u737b\uff0c\u76ee\u524d\u5728\u6750\u6599\u5316\u5b78\u3001\u751f\u7269\u88fd\u85e5\u548c\u5316\u5b78\u751f\u7269\u5b78\u7684\u8af8\u591a\u9818\u57df\u4e0a\u4e5f\u5c55\u73fe\u91cd\u5927\u5ee3\u6cdb\u7684\u61c9\u7528\u6027\u3002<\/p>\n<p>\u91dd\u5c0d\u300c\u751f\u7269\u6b63\u4ea4\u6027\u5316\u5b78\u300d\u7684\u5b9a\u7fa9\uff0c\u758a\u6c2e\u548c\u7094\u985e\u9019\u5169\u7a2e\u5316\u5b78\u5b98\u80fd\u57fa\u7686\u4e0d\u5e38\u898b\u65bc\u8907\u96dc\u751f\u7269\u4ee3\u8b1d\u7cfb\u7d71\uff0c\u4e00\u822c\u8a8d\u70ba\u5c6c\u65bc\u300c\u751f\u7269\u6027\u6b63\u4ea4\u5b98\u80fd\u57fa\u300d\uff0c\u4e0d\u6703\u5e72\u64fe\u6216\u53c3\u8207\u81ea\u7136\u751f\u7269\u4ee3\u8b1d\u53cd\u61c9\u6a5f\u5236\uff0c\u5361\u7f85\u7433\u00b7\u8c9d\u723e\u6258\u897f\u7814\u7a76\u5718\u968a\u5229\u7528\u5316\u5b78\u5408\u6210\u542b\u758a\u6c2e\u5b98\u80fd\u57fa\u7684\u55ae\u91a3\u57fa\u8cea(\u7c21\u7a31\u91a3\u5316\u5b78\u63a2\u91dd)\uff0c\u4e26\u5de7\u5999\u5229\u7528\u7d30\u80de\u5167\u6240\u5b58\u5728\u7684\u91a3\u9175\u7d20\u8f49\u79fb\u9176\u4ee3\u8b1d\u6a5f\u5236\u7684\u5c07\u91a3\u63a2\u91dd\u5f15\u5165\u76f8\u95dc\u7684\u751f\u7269\u5206\u5b50\uff0c\u81ea\u7136\u5f62\u6210\u91a3\u86cb\u767d\u6216\u91a3\u8102\u8cea(\u7b56\u7565\u904e\u7a0b\u7c21\u7a31MOE\uff0cmetabolic oligosaccharide engineering)[3]\uff0c\u901a\u904e\u5f8c\u7e8cCuAAC\u53cd\u61c9\u4ee5\u76f8\u5c0d\u61c9\u7684\u7094\u985e\u4fee\u98fe\u7684\u6709\u6a5f\u5c0f\u5206\u5b50\u6216\u87a2\u5149\u67d3\u6599\u9032\u884c\u5316\u5b78\u4fee\u98fe\u6216\u53ef\u8996\u5316\u87a2\u5149\u6a19\u5b9a\uff0c\u85c9\u6b64\u7372\u53d6\u86cb\u767d\u8cea\u9032\u884c\u5f8c\u4fee\u98fe\u91a3\u57fa\u5316\u4f5c\u7528\u8a0a\u606f\u53ca\u5176\u4e0d\u540c\u751f\u7269\u5206\u5b50\u9593\u7684\u4ea4\u4e92\u4f5c\u7528\u7684\u8b8a\u5316\u4fe1\u606f\uff0c\u5982\u5716\u4e00\u6240\u793a\u3002\u7531\u65bc\u76ee\u524d\u7814\u7a76\u986f\u793a\u7d30\u80de\u5167\u751f\u7269\u5206\u5b50\u7684\u91a3\u57fa\u5f8c\u4fee\u98fe\u5316\u4e0d\u53ea\u53ef\u8abf\u63a7\u7d30\u80de\u529f\u80fd\uff0c\u540c\u6642\u8207\u764c\u7d30\u80de\u8f49\u79fb\u3001\u5fc3\u8840\u7ba1\u75be\u75c5\u4ee5\u53ca\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u7b49\u4eba\u985e\u91cd\u5927\u75be\u75c5\u606f\u606f\u76f8\u95dc\uff0c\u4e26\u4e14\u4eba\u9ad4\u4e2d\u86cb\u767d\u8cea\u534a\u6578\u4ee5\u4e0a\u90fd\u5e36\u6709\u91a3\u5206\u5b50\uff0c\u4e86\u89e3\u9019\u4e9b\u91a3\u5206\u5b50\u4fee\u98fe\u53ca\u5176\u5728\u7d30\u80de\u751f\u7269\u4e0a\u626e\u6f14\u89d2\u8272\u4fbf\u65e5\u76ca\u91cd\u8981\uff0c\u9019\u7a2e\u7d50\u5408\u751f\u7269\u91a3\u4ee3\u8b1d(MOE)\u53ca\u5316\u5b78\u53cd\u61c9(click chemistry)\u7684\u751f\u7269\u5206\u5b50\u4fee\u98fe\u7684\u7b56\u7565\uff0c\u4f7f\u5f97\u8aaa\u660e\u8907\u96dc\u91a3\u5316\u751f\u7269\u5927\u5206\u5b50\u8207\u751f\u547d\u7d30\u80de\u9ad4\u7cfb\u4e4b\u76f8\u4e92\u95dc\u4fc2\u7684\u7814\u7a76\u5728\u8fd1\u5e74\u4f86\u53d6\u5f97\u660e\u986f\u9032\u5c55\u6210\u679c\u3002\u5728\u6b64\u540c\u6642\uff0c\u4ee5\u4e00\u50f9\u9285\u96e2\u5b50\u50ac\u5316[Cu(I)-catalyzed]\u70ba\u57fa\u790e\u7684CuCCA\u53cd\u61c9\u96d6\u7136\u53ef\u5ee3\u6cdb\u7528\u65bc\u5316\u5b78\u751f\u7269\u5b78\u7814\u7a76\uff0c\u4f46\u7531\u65bc\u96e2\u5b50\u7d30\u80de\u6bd2\u6027\uff0c\u5c0e\u81f4\u5728\u6d3b\u7d30\u80de\u6216\u751f\u7269\u500b\u9ad4\u4e0a\u5316\u5b78\u6a19\u5b9a\u7684\u61c9\u7528\u4e0a\u53d7\u5230\u6975\u5927\u9650\u5236\uff0c\u4e26\u4e0d\u5b8c\u7136\u7b26\u5408\u751f\u7269\u6b63\u4ea4\u6027\u5316\u5b78\u7684\u8981\u6c42\u3002\u6709\u9452\u65bc\u6b64\uff0c\u8a31\u591a\u7814\u7a76\u5718\u968a\u8a2d\u8a08\u5404\u7a2e\u6709\u6a5f\u914d\u9ad4(ligand)\u8a66\u5716\u8207\u4e00\u50f9\u9285\u96e2\u5b50\u5f62\u6210\u7a69\u5b9a\u932f\u5408\u7269\uff0c\u76ee\u7684\u5728\u6e1b\u5c11\u9285\u96e2\u5b50\u4f7f\u7528\u91cf\uff0c\u4e26\u4e14\u53ef\u5728\u6d3b\u9ad4\u7cfb\u7d71\u7684\u61c9\u7528\u4e0a\u53d6\u5f97\u91cd\u5927\u9032\u5c55(\u7b56\u7565\u904e\u7a0b\u7c21\u7a31LACuAAC\uff0cLigand-assitsted Cu-catalyzed Azide-Alkyne Cycloaddition)[4]\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u5361\u7f85\u7433\u00b7\u8c9d\u723e\u6258\u897f\u7814\u7a76\u5718\u968a\u5247\u610f\u8b58\u5230\u4ee5\u9285\u96e2\u5b50\u50ac\u5316\u70ba\u57fa\u790e\u7684CuAAC\u53cd\u61c9\u4e26\u7121\u6cd5\u5168\u7136\u6eff\u8db3\u6d3b\u9ad4\u7cfb\u7d71\u5316\u5b78\u6a19\u5b9a\u7684\u9700\u6c42\uff0c\u56e0\u800c\u63d0\u51fa\u5229\u7528\u74b0\u7094\u672c\u8eab\u9ad8\u5f35\u529b\u6027\u8cea\u4fc3\u4f7f\u758a\u6c2e\u7121\u9808\u9285\u96e2\u5b50\u53c3\u8207\u4e0b\u4fbf\u53ef\u5728\u6eab\u548c\u53cd\u61c9\u689d\u4ef6\u4e2d\u8207\u74b0\u7094\u751f\u6210\u4e09\u5511\u985e\u5316\u5408\u7269\u7684\u5316\u5b78\u53cd\u61c9\u7b56\u7565\uff0c\u7c21\u7a31SPAAC (Strain-Promoted Azide-Alkyne Cycloaddition)\uff0c\u76ee\u524d\u5df2\u6709\u5404\u5f0f\u5404\u6a23\u516b\u54e1\u74b0\u7094\u985e\u884d\u751f\u7269\u904b\u7528\u65bc\u5404\u985e\u578b\u6d3b\u9ad4\u7cfb\u7d71\u751f\u7269\u5206\u5b50\u6a19\u5b9a\u4e0a[5]\u3002<\/p>\n<p>&nbsp;<\/p>\n<div id=\"attachment_20252\" style=\"width: 986px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20252\" class=\"wp-image-20252\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2019\/10\/3-1\u6f2b\u6b65\u79d1\u7814-\u3010\u672c\u671f\u5c08\u6b04\u3011\u5316\u5b78\u6240-\u5716\u72471-1024x569.jpg\" alt=\"\" width=\"976\" height=\"543\" srcset=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2019\/10\/3-1\u6f2b\u6b65\u79d1\u7814-\u3010\u672c\u671f\u5c08\u6b04\u3011\u5316\u5b78\u6240-\u5716\u72471-1024x569.jpg 1024w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2019\/10\/3-1\u6f2b\u6b65\u79d1\u7814-\u3010\u672c\u671f\u5c08\u6b04\u3011\u5316\u5b78\u6240-\u5716\u72471-300x167.jpg 300w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2019\/10\/3-1\u6f2b\u6b65\u79d1\u7814-\u3010\u672c\u671f\u5c08\u6b04\u3011\u5316\u5b78\u6240-\u5716\u72471-768x427.jpg 768w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2019\/10\/3-1\u6f2b\u6b65\u79d1\u7814-\u3010\u672c\u671f\u5c08\u6b04\u3011\u5316\u5b78\u6240-\u5716\u72471-1080x600.jpg 1080w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2019\/10\/3-1\u6f2b\u6b65\u79d1\u7814-\u3010\u672c\u671f\u5c08\u6b04\u3011\u5316\u5b78\u6240-\u5716\u72471-256x142.jpg 256w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2019\/10\/3-1\u6f2b\u6b65\u79d1\u7814-\u3010\u672c\u671f\u5c08\u6b04\u3011\u5316\u5b78\u6240-\u5716\u72471-350x195.jpg 350w, 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\/>\n[1]. Sletten, Ellen M.; Bertozzi, C. R. Angew. Chem. Int. Ed. 2009, 48, 6974\u20136998. \u201cBioorthogonal chemistry: Fishing for selectivity in a sea of functionality\u201d<br \/>\n[2]. Rostovtsev, V. V.; Green, L. G.; Fokin, V. V.; Sharpless, K. B. Angew. Chem. Int. Ed. 2002, 41, 2596\u22122599. \u201cA stepwise Huisgen cycloaddition process: Copper(I)-catalyzed regioselective \u201cligation\u201d of azides and terminal alkynes.\u201d<br \/>\n[3]. Laughlin, S. T.; Bertozzi, C. R. Nat. Protoc. 2007, 2, 2930\u22122944. \u201cMetabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation.\u201d<br \/>\n[4]. del Amo, D. S.; Wang, W.; Jiang, H.; Besanceney, C.; Yan, A. C.; Levy, M.; Liu, Y.; Marlow, F. L.; Wu, P. J. Am. Soc. Chem. 2010, 132, 16893\u201316899. \u201cBiocompatible copper(I) catalysts for in vivo Imaging of glycans.\u201d<br \/>\n[5]. Jewett, J. C.; Bertozzi, C. R. Chem. Soc. Rev. 2010, 39, 1272\u22121279. \u201cCu-free click cycloaddition reactions in chemical biology.\u201d<br \/>\n[6]. Shie, J.-J.; Liu, Y.-C.; Lee, Y.-M.; Lim, C.; Fang, J.-M.; Wong, C.-H. J. Am. Chem. Soc. 2014, 136, 9953\u20129961. \u201cAn azido-BODIPY probe for glycosylation: Initiation of strong fluorescence upon triazole formation.\u201d<br \/>\n[7]. Shie, J.-J.; Liu, Y.-C.; Hsiao, J.-C.; Fang, J.-M.; Wong, C.-H. Chem. Commun. 2017, 53, 1490\u20121493. \u201cA cell-permeable and triazole-forming fluorescence probe for glycoconjugate imaging in live cells.\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9577\u671f\u4ee5\u4f86\uff0c\u751f\u7269\u5b78\u5bb6\u6e34\u671b\u85c9\u7531\u767c\u5c55\u5404\u7a2e\u7814\u7a76\u7b56\u7565\u671f\u5f85\u4e86\u89e3\u751f\u7269\u6210\u9577\u4ee3\u8b1d\u6a5f\u5236\uff0c\u5f9e\u4e2d\u627e\u51fa\u751f\u547d\u7a0b\u5e8f\u7684\u5967\u5999\uff0c\u4ee5\u53ca\u91dd\u5c0d\u75be\u75c5\u627e\u51fa\u56e0 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":20252,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[991,983],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/20251"}],"collection":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/comments?post=20251"}],"version-history":[{"count":2,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/20251\/revisions"}],"predecessor-version":[{"id":20255,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/20251\/revisions\/20255"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media\/20252"}],"wp:attachment":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media?parent=20251"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/categories?post=20251"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/tags?post=20251"}],"curies":[{"name":"wp","href":"https:\/api.w.org\/{rel}","templated":true}]}}