\u86cb\u767d\u8cea\u662f\u57fa\u56e0\u8868\u73fe\u7684\u7d42\u7522\u7269\uff0c\u751f\u7269\u9ad4\u5167\u9032\u884c\u53cd\u61c9\u591a\u4ee5\u86cb\u767d\u8cea\u70ba\u4e3b\uff0c\u800c\u975eDNA\u6216RNA\uff0c\u6240\u4ee5\u8981\u4e86\u89e3\u751f\u547d\u7684\u5967\u79d8\uff0c\u5fc5\u9808\u8981\u5c0d\u86cb\u767d\u8cea\u6709\u6df1\u5165\u5730\u4e86\u89e3\u3002\u6b64\u5916\uff0c\u81e8\u5e8a\u7528\u85e5\u7269\u4e5f\u662f\u900f\u904e\u8207\u86cb\u767d\u8cea\u7d50\u5408\uff0c\u4f86\u9054\u5230\u6cbb\u7642\u7684\u76ee\u7684\uff0c\u56e0\u6b64\u86cb\u767d\u8cea\u7814\u7a76\u5c0d\u660e\u77ad\u85e5\u7269\u7684\u6d3b\u6027\u8207\u65b0\u85e5\u7684\u958b\u767c\u6975\u70ba\u91cd\u8981\u3002<\/p>\n
\u7814\u7a76\u86cb\u767d\u8cea\u7684\u4e3b\u8981\u6280\u8853\u74f6\u9838\uff0c\u662f\u5982\u4f55\u53d6\u5f97\u91cf\u8db3\u8cea\u7d14\u7684\u86cb\u767d\u3002\u76ee\u524d\u6700\u5e38\u7528\u7684\u65b9\u6cd5\u662f\u57fa\u56e0\u91cd\u7d44\u6280\u8853\uff0c\u5148\u5efa\u69cb\u6b32\u8868\u9054\u6a19\u7684\u86cb\u767d\u7684\u8f09\u9ad4\uff0c\u5c07\u8f09\u9ad4\u9001\u5165\u5bc4\u4e3b\u7d30\u80de\uff0c\u5f8c\u8005\u6703\u8aa4\u8a8d\u8f09\u9ad4\u4e0a\u7684\u6a19\u7684\u86cb\u767d\u57fa\u56e0\u70ba\u81ea\u5df1\u7684\u57fa\u56e0\uff0c\u5927\u91cf\u8868\u9054\u51fa\u6a19\u7684\u86cb\u767d\uff0c\u7136\u5f8c\u7d93\u7d14\u5316\u6b65\u9a5f\u53d6\u5f97\u6a19\u7684\u86cb\u767d\u3002\u76ee\u524d\u76f8\u95dc\u6280\u8853\u5df2\u9ad8\u5ea6\u767c\u5c55\uff0c\u5e7e\u4e4e\u9054\u5230\u4eba\u4eba\u7686\u53ef\u505a\u7684\u7c21\u6613\u7a0b\u5ea6\u3002\u53ef\u60dc\uff0c\u5728\u5be6\u969b\u64cd\u4f5c\u6642\uff0c\u7d50\u679c\u7d93\u5e38\u4e0d\u5982\u9810\u671f\u3002\u86cb\u767d\u8cea\u4f9d\u7269\u7406\u7279\u6027\u53ef\u5206\u6c34\u6eb6\u6027\u8207\u8102\u6eb6\u6027\u5169\u985e\u3002\u8102\u6eb6\u6027\u86cb\u767d\u5fc5\u9808\u5d4c\u9472\u65bc\u6cb9\u6027\u7684\u7d30\u80de\u5916\u819c\u6216\u5404\u7a2e\u80de\u5668\u819c\uff0c\u7531\u65bc\u7d30\u80de\u88e1\u819c\u7684\u7e3d\u8868\u9762\u7a4d\u6709\u9650\uff0c\u5927\u91cf\u8868\u9054\u6642\uff0c\u591a\u6578\u8102\u6eb6\u6027\u86cb\u767d\u7121\u6cd5\u9032\u5165\u819c\u5167\uff0c\u7d93\u758f\u6c34\u6027\u4f5c\u7528\uff0c\u76f8\u805a\u6210\u7121\u6d3b\u6027\u7684\u56fa\u9ad4\u5718\uff0c\u5373\u6240\u8b02\u300c\u5305\u6db5\u9ad4\u300d\u3002\u6c34\u6eb6\u6027\u86cb\u767d\uff0c\u96d6\u4e0d\u9808\u5d4c\u65bc\u819c\u5167\uff0c\u5927\u91cf\u8868\u9054\u6642\uff0c\u4e5f\u5e38\u5f62\u6210\u5305\u6db5\u9ad4\u3002\u76f8\u95dc\u7814\u7a76\u986f\u793a\uff0c\u9ad8\u905480\uff05\u6240\u8b02\u7684\u6c34\u6eb6\u6027\u86cb\u767d\uff0c\u4e0d\u6613\u5927\u91cf\u8868\u9054\u6210\u80fd\u6eb6\u65bc\u6c34\u7684\u91cd\u7d44\u86cb\u767d\uff0c\u9019\u662f\u73fe\u4ee3\u751f\u7269\u8207\u751f\u7269\u79d1\u6280\u91cd\u8981\u8ab2\u984c\u4e4b\u4e00\u3002<\/p>\n
\u89e3\u6c7a\u4e0a\u8ff0\u554f\u984c\uff0c\u65b9\u6cd5\u4e4b\u4e00\u662f\u878d\u5408\u86cb\u767d\u6280\u8853\uff08Fusion Protein Approach\uff09\u3002\u6b64\u65b9\u6cd5\u4fc2\u5c07\u651c\u5e36\u86cb\u767d\uff08Carrier\uff09\u7684\u57fa\u56e0\u8207\u6a19\u7684\u86cb\u767d\u7684\u57fa\u56e0\uff0c\u4ee5\u57fa\u56e0\u91cd\u7d44\u6280\u8853\u7d50\u5408\u70ba\u4e00\uff0c\u8868\u9054\u51fa\u300c\u651c\u5e36\u86cb\u767d+\u6a19\u7684\u86cb\u767d\u300d\u524d\u5f8c\u63a5\u5408\u7684\u878d\u5408\u86cb\u767d\u3002\u878d\u5408\u86cb\u767d\u56e0\u651c\u5e36\u86cb\u767d\u7684\u52a0\u6301\u8f14\u52a9\uff0c\u8868\u73fe\u91cf\u8207\u6c34\u6eb6\u6027\u90fd\u53ef\u589e\u52a0\u3002\u878d\u5408\u86cb\u767d\u5728\u8a2d\u8a08\u6642\uff0c\u6703\u5728\u651c\u5e36\u86cb\u767d\u8207\u6a19\u7684\u86cb\u767d\u9593\uff0c\u52a0\u5165\u4e00\u6bb5\u7279\u5225\u7684\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u53ef\u88ab\u86cb\u767d\u6c34\u89e3\u9176\u5c08\u4e00\u6027\u7684\u8fa8\u8a8d\u8207\u5207\u5272\uff0c\u9054\u5230\u5206\u96e2\u5169\u8005\u7684\u76ee\u7684\uff0c\u518d\u7d93\u4e00\u8f2a\u7d14\u5316\u6b65\u9a5f\uff0c\u5373\u53ef\u53d6\u5f97\u6a19\u7684\u86cb\u767d\u3002\u7531\u904e\u53bb\u8a31\u591a\u751f\u5316\u5b78\u8005\u5be6\u9a57\u7d93\u9a57\u5f97\u77e5\uff0c\u540c\u4e00\u651c\u5e36\u86cb\u767d\u5c0d\u4e0d\u540c\u7684\u6a19\u7684\u86cb\u767d\uff0c\u6548\u679c\u6709\u5f88\u5927\u5dee\u7570\u3002\u76ee\u524d\u6709\u5341\u5e7e\u7a2e\u4e0d\u540c\u7684\u651c\u5e36\u86cb\u767d\u53ef\u4f9b\u9078\u64c7\uff0c\u56e0\u6b64\u5c0d\u540c\u4e00\u6a19\u7684\u86cb\u767d\u800c\u8a00\uff0c\u6bd4\u8f03\u4fdd\u96aa\u7684\u8fa6\u6cd5\u5c31\u662f\u591a\u8a66\u5e7e\u500b\u4e0d\u540c\u7684\u651c\u5e36\u86cb\u767d\u3002\u8981\u505a\u5230\u9019\u9ede\u5f88\u5bb9\u6613\uff0c\u53ea\u9700\u5c07\u6a19\u7684\u86cb\u767d\u57fa\u56e0\uff0c\u5229\u7528\u91cd\u7d44\u57fa\u56e0\u65b9\u6cd5\uff0c\u5206\u5225\u7f6e\u5165\u542b\u6709\u4e0d\u540c\u651c\u5e36\u86cb\u767d\u7684\u8f09\u9ad4\u5373\u53ef\u3002\u53ef\u662f\u7576\u5be6\u969b\u8981\u540c\u6b65\u9078\u6b96\u8f09\u9ad4\u6642\uff0c\u7d93\u5e38\u5148\u8981\u82b1\u4e00\u756a\u529f\u592b\u9078\u64c7\u9069\u7576\u7684\u9650\u5236\u9176\uff0c\u53bb\u5207\u5272\u4e0d\u540c\u8f09\u9ad4\u8207\u6a19\u7684\u86cb\u767d\u7684DNA\uff0c\u7136\u5f8c\u624d\u80fd\u9032\u884c\u57fa\u56e0\u91cd\u7d44\u5de5\u4f5c\u3002\u70ba\u4e86\u514d\u9664\u9078\u64c7\u9650\u5236\u9176\u7684\u7e41\u7463\uff0c\u7b46\u8005\u767c\u660e\u4e00\u500b\u65b0\u65b9\u6cd5\uff0c\u53ea\u7528\u5169\u500b\u4efb\u610f\u9078\u5b9a\u7684\u9650\u5236\u9176\uff08\u5982EcoRI\u8207XhoI\uff09\uff0c\u61c9\u7528\u975e\u5e73\u6574\u7aef\u9ede\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9\uff08Sticky-End PCR\uff09\uff0c\u5c07\u4efb\u4f55\u6a19\u7684\u86cb\u767d\u57fa\u56e0\u7684\u5169\u7aef\uff0c\u5206\u5225\u88fd\u51faEcoRI\u6216XhoI\u4e4b\u975e\u5e73\u6574\u7aef\u9ede\uff08\u57161A\uff09\uff0c\u518d\u4ee5\u56fa\u5b9a\u65b9\u5411\uff0c\u540c\u6b65\u7f6e\u5165\u4e0d\u540c\u8f09\u9ad4\u3002\u6b64\u65b0\u65b9\u6cd5\u7684\u539f\u7406\u8207\u6b65\u9a5f\u90fd\u5f88\u7c21\u55ae\uff0c\u5f88\u5feb\u5c31\u5728\u5be6\u9a57\u5ba4\u9a57\u8b49\u6210\u529f\uff0c\u4e26\u5efa\u7acb\u4e86\u9ad8\u6548\u7387\u6c34\u6eb6\u6027\u86cb\u767d\u8cea\u7be9\u9078\u6280\u8853\u5e73\u53f0\uff0c\u65bc2002\u5e74\u767c\u8868\u5728Protein Science\u671f\u520a\u3002<\/p>\n
\u57161\u3001\u975e\u5e73\u6574\u7aef\u9ede\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9\u61c9\u7528\u65bc\u57fa\u56e0\u91cd\u7d44\u65b9\u6cd5\u3002\uff08A\uff09\u5229\u7528\u5169\u5c0d\u5f15\u5b50\u5206\u5225\u9032\u884c\u5169\u500b\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9\uff0c\u7136\u5f8c\u5c07\u5169\u500b\u7522\u7269\u7b49\u91cf\u6df7\u5408\uff0cDNA\u76845\u2019\u7aef\u5229\u7528T4\u591a\u6838\u82f7\u9178\u6fc0\u9176\u52a0\u4ee5\u78f7\u9178\u5316\uff0c95oC\u52a0\u71b15\u5206\u9418\uff0c\u4f7f\u96d9\u80a1DNA\u5206\u96e2\u6210\u55ae\u80a1DNA\uff0c65oC\u975c\u7f6e5\u5206\u9418\uff0c\u4f7f\u55ae\u80a1DNA\u53c8\u56de\u5fa9\u6210\u96d9\u80a1DNA\u3002\u7d50\u679c\u6703\u670925\uff05\u96d9\u80a1DNA\uff0c\u5176\u5169\u7aef\u5206\u5225\u7522\u751fEcoRI\u8207XhoI\u7684\u975e\u5e73\u6574\u7aef\u9ede\uff0c\u53ef\u76f4\u63a5\u8207\u8f09\u9ad4\u9032\u884c\u63a5\u5408\u3002\uff08B\uff09\u5982\uff08A\uff09\u6240\u8ff0\uff0c\u56e0SnaBI\u5207\u5272\u51fa\u5e73\u6574\u7aef\u9ede\uff0c\u4f9d\u5716\u5b8c\u6210\u53cd\u61c9\u6b65\u9a5f\u5f8c\uff0c\u670950\uff05\u96d9\u80a1DNA\uff0c\u4e00\u7aef\u6709SnaBI\u7684\u5e73\u6574\u7aef\u9ede\uff0c\u53e6\u4e00\u7aef\u662fXhoI\u7684\u975e\u5e73\u6574\u7aef\u9ede\u3002P2\u3001P1\u8207P1\u2019\u4ee3\u8868\u6c28\u57fa\u9178\u4f4d\u7f6e\uff0c\u8a73\u7d30\u89e3\u91cb\u898b\u57162\u3002<\/p>\n
\u7531\u65bc\u6b64\u6cd5\u9069\u7528\u65bc\u4efb\u4f55\u57fa\u56e0\uff0c\u672c\u6240\u738b\u60e0\u921e\u6240\u9577\u5c07\u5176\u7d0d\u5165\u570b\u79d1\u6703\u6240\u88dc\u52a9\u7684\u9ad8\u6548\u7387\u86cb\u767d\u8ceaX\u5149\u7d50\u69cb\u6838\u5fc3\u5be6\u9a57\u5ba4\u8a08\u756b\u4e2d\u3002\u5f8c\u4f86\uff0c\u57fa\u56e0\u9ad4\u8a08\u756b\u8fa6\u516c\u5ba4\u5efa\u8b70\u738b\u6240\u9577\u5c07\u6b64\u65b0\u6280\u8853\u53e6\u5916\u6210\u7acb\u9ad8\u6548\u7387\u86cb\u767d\u8cea\u8868\u9054\u6838\u5fc3\u5be6\u9a57\u5ba4\u3002\u4e09\u5e74\u591a\u4f86\uff0c\u8a72\u6838\u5fc3\u5be6\u9a57\u5ba4\u670d\u52d9\u904e\u570b\u5167\u6578\u5341\u500b\u5be6\u9a57\u5ba4\uff0c\u5b8c\u6210\u8fd1\u4e09\u767e\u4ef6\u6c34\u6eb6\u6027\u86cb\u767d\u8cea\u7be9\u9078\u5de5\u4f5c\uff0c\u5176\u4e2d\u5e7e\u500b\u5be6\u9a57\u5ba4\u5df2\u767c\u8868\u76f8\u95dc\u6210\u679c\u3002\u6211\u5011\u4e5f\u900f\u904e\u6280\u8853\u8f49\u79fb\u65b9\u5f0f\uff0c\u5728\u570b\u5167\u5916\u63a8\u5ee3\u6b64\u6280\u8853\u5e73\u53f0\uff0c\u570b\u5167\u76ee\u524d\u6709\u8d85\u904e40\u500b\u5be6\u9a57\u5ba4\u6216\u7814\u7a76\u6a5f\u69cb\u63a5\u53d7\u6280\u8f49\uff0c\u570b\u5916\u6280\u8f49\u5c0d\u8c61\u5305\u62ec\u829d\u52a0\u54e5\u5927\u5b78\u3001\u7d10\u7d04\u5927\u5b78\u3001\u4e39\u9ea5Aarhus\u5927\u5b78\u3001\u8377\u862dGroningen\u5927\u5b78\u3001\u65e5\u672c\u6771\u4eac\u5927\u5b78\u7b49\u6578\u500b\u5be6\u9a57\u5ba4\u3002\u53bb\u5e74\u6211\u5011\u4e5f\u53d7\u9080\u70ba\u7f8e\u570b\u51b7\u6cc9\u6e2f\u5be6\u9a57\u5ba4\u6240\u51fa\u7248\u7684\u86cb\u767d\u8cea\u9ad4\u5b78\u6280\u8853\u5c08\u66f8\uff0c\u64b0\u5beb\u4e00\u7ae0\u5c08\u6587\uff0c\u66f4\u8a73\u7d30\u8aaa\u660e\u6b64\u6280\u8853\u7684\u8a2d\u8a08\u8207\u64cd\u4f5c\u6d41\u7a0b\u3002<\/p>\n
\u96d6\u7136\u4ee5\u4e0a\u65b9\u6cd5\u53ef\u5feb\u901f\u7be9\u9078\u80fd\u7522\u751f\u5927\u91cf\u6c34\u6eb6\u6027\u86cb\u767d\u7684\u8f09\u9ad4\uff0c\u672c\u8cea\u4e0a\u4ecd\u7121\u6cd5\u907f\u514d\u878d\u5408\u86cb\u767d\u6280\u8853\u65e2\u6709\u7684\u7f3a\u5931\u3002\u8a72\u65b9\u6cd5\u904e\u7a0b\u5197\u9577\u4e14\u6602\u8cb4\uff0c\u8981\u5148\u7d14\u5316\u878d\u5408\u86cb\u767d\uff0c\u7136\u5f8c\u7528\u6602\u8cb4\u7684\u86cb\u767d\u6c34\u89e3\u9176\u5c07\u878d\u5408\u86cb\u767d\u5207\u958b\uff0c\u518d\u7d93\u7b2c\u4e8c\u8f2a\u86cb\u767d\u8cea\u7d14\u5316\u6b65\u9a5f\uff0c\u624d\u53d6\u5f97\u6a19\u7684\u86cb\u767d\u3002\u6709\u4e9b\u6a19\u7684\u86cb\u767d\u4e00\u65e6\u8207\u651c\u5e36\u86cb\u767d\u5206\u96e2\u5f8c\uff0c\u7acb\u523b\u5931\u53bb\u6c34\u6eb6\u6027\u767c\u751f\u6c88\u6bbf\uff0c\u56e0\u800c\u524d\u529f\u76e1\u68c4\u3002\u53e6\u4e00\u500b\u5927\u554f\u984c\u662f\u878d\u5408\u86cb\u767d\u7d93\u6c34\u89e3\u9176\u5207\u5272\u5f8c\uff0c\u7522\u7269\u5e38\u6703\u6bd4\u539f\u614b\u86cb\u767d\u591a\u51fa\u5e7e\u500b\u984d\u5916\u7684\u6c28\u57fa\u9178\u3002\u5f8c\u8005\u5f62\u6210\u539f\u56e0\uff0c\u662f\u56e0\u5fc5\u9808\u65bc\u6a19\u7684\u86cb\u767d\u8207\u651c\u5e36\u86cb\u767d\u57fa\u56e0\u9593\uff0c\u5916\u52a0\u5165\u9650\u5236\u9176\u8207\u86cb\u767d\u8cea\u6c34\u89e3\u9176\u6240\u80fd\u8fa8\u8b58\u7684\u7279\u5b9a\u5e8f\u5217\u3002\u9019\u4e9b\u591a\u51fa\u4f86\u7684\u6c28\u57fa\u9178\uff0c\u6709\u4e9b\u6703\u5f71\u97ff\u6a19\u7684\u86cb\u767d\u7684\u751f\u5316\u6d3b\u6027\u6216\u7d50\u69cb\u7a69\u5b9a\u6027\uff0c\u5373\u4fbf\u5c0d\u5169\u8005\u90fd\u6c92\u6709\u5f71\u97ff\uff0c\u4e5f\u5927\u5927\u964d\u4f4e\u4e86\u8a72\u86cb\u767d\u4e4b\u61c9\u7528\u50f9\u503c\u3002\u56e0\u70ba\u81e8\u5e8a\u7528\u7684\u86cb\u767d\u8cea\u85e5\u6216\u88fd\u5291\uff0c\u57fa\u672c\u8981\u6c42\u4e4b\u4e00\uff0c\u5c31\u662f\u5176\u6c28\u57fa\u9178\u5e8f\u5217\u8207\u7d44\u6210\u5fc5\u9808\u8207\u539f\u614b\u86cb\u767d\u5b8c\u5168\u76f8\u540c\uff0c\u5426\u5247\u5c31\u5f97\u5927\u8cbb\u5468\u7ae0\u5730\u63d0\u51fa\u751f\u7406\u8207\u75c5\u7406\u7814\u7a76\u6578\u64da\uff0c\u8b49\u660e\u591a\u51fa\u4f86\u7684\u6c28\u57fa\u9178\u5c0d\u4eba\u9ad4\u7121\u5bb3\uff0c\u4e5f\u4e0d\u6703\u767c\u751f\u514d\u75ab\u6392\u65a5\u7b49\u554f\u984c\u3002\u57fa\u65bc\u4ee5\u4e0a\u8003\u91cf\uff0c\u878d\u5408\u86cb\u767d\u6280\u8853\u904e\u53bb\u5728\u751f\u6280\u5de5\u696d\u4e26\u4e0d\u53d7\u9752\u775e\u3002<\/p>\n
\u5728\u672c\u6240\u738b\u6240\u9577\u652f\u6301\u4e0b\uff0c\u6211\u5011\u6700\u8fd1\u6210\u529f\u7814\u767c\u51fa\u4e00\u5957\u65b0\u6280\u8853\uff0c\u53ef\u6539\u5584\u4e0a\u8ff0\u7f3a\u5931\u3002\u6b64\u65b9\u6cd5\u662f\u5728\u5bc4\u4e3b\u7d30\u80de\u5167\uff0c\u540c\u6642\u8868\u9054\u878d\u5408\u86cb\u767d\u8207\u7159\u8349\u8755\u523b\u75c5\u6bd2\u86cb\u767d\u6c34\u89e3\u9176\uff08tobacco etch virus protease\uff0c\u7c21\u7a31TEVP\uff09\uff0cTEVP\u53ef\u76f4\u63a5\u5728\u7d30\u80de\u5167\u5207\u5272\u878d\u5408\u86cb\u767d\uff0c\u56e0\u6b64\u7121\u9700\u7d93\u904e\u4efb\u4f55\u7d14\u5316\u6b65\u9a5f\uff0c\u5373\u53ef\u6aa2\u9a57\u6a19\u7684\u86cb\u767d\u7684\u8868\u73fe\u91cf\u8207\u6c34\u6eb6\u6027\u3002\u9019\u9805\u65b0\u6280\u8853\uff0c\u4ecd\u63a1\u7528\u975e\u5e73\u6574\u7aef\u9ede\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9\u7684\u65b9\u6cd5\uff0c\u53ea\u7528\u5169\u500b\u7279\u5b9a\u7684\u9650\u5236\u9176\uff08SnaBI\u8207XhoI\uff1b\u57161B\uff09\uff0c\u5c31\u53ef\u5c07\u4efb\u4f55\u6a19\u7684\u86cb\u767d\u7684\u57fa\u56e0\u5e73\u884c\u9078\u6b96\u5165\u591a\u500b\u4e0d\u540c\u8f09\u9ad4\u3002SnaBI\u9650\u5236\u9176\u7684\u9078\u7528\u662f\u975e\u5e38\u5225\u51fa\u5fc3\u88c1\u7684\u8a2d\u8a08\uff0c\u7d50\u679c\u80fd\u4f7f\u7522\u51fa\u7684\u6a19\u7684\u86cb\u767d\uff0c\u4e0d\u50c5\u5b8c\u5168\u6c92\u6709\u591a\u51fa\u4efb\u4f55\u6c28\u57fa\u9178\u5e8f\u5217\uff08\u5373\u539f\u614b\u86cb\u767d\uff09\uff0c\u4e5f\u53ef\u4f9d\u9700\u6c42\u96a8\u610f\u6539\u8b8a\u524d\u5e7e\u500b\u6c28\u57fa\u9178\u5e8f\u5217\u3002\u6b64\u7cfb\u7d71\u53e6\u4e00\u500b\u512a\u9ede\u662f\uff0c\u53ef\u61c9\u7528\u5728\u591a\u7a2e\u5bc4\u4e3b\u7d30\u80de\uff0c\u5982\u5927\u8178\u687f\u83cc\u3001\u9175\u6bcd\u83cc\u8207\u52d5\u7269\u7d30\u80de\u5747\u53ef\uff0c\u56e0\u70baTEVP\u5728\u9019\u4e9b\u7d30\u80de\u8868\u9054\u5f8c\uff0c\u90fd\u6709\u6d3b\u6027\u3002\u5be6\u969b\u61c9\u7528\u6642\uff0c\u53ef\u4ee5trans\u6216cis\u5169\u7a2e\u65b9\u5f0f\u9032\u884c\uff0c\u524d\u8005\u662f\u5728\u5bc4\u4e3b\u7d30\u80de\u8868\u9054\u300c\u651c\u5e36\u86cb\u767d-rsTEV-\u6a19\u7684\u86cb\u767d\u300d\u8207TEVP\u5169\u500b\u86cb\u767d\uff0crsTEV\u4ee3\u8868TEVP\u6240\u8fa8\u8b58\u5207\u5272\u7684\u7279\u5b9a\u6c28\u57fa\u9178\u5e8f\u5217\uff08\u57162A\uff09\u3002cis\u65b9\u6cd5\u5247\u8868\u9054\u4e00\u500b\u8f03\u9577\u7684\u878d\u5408\u86cb\u767d\uff08\u651c\u5e36\u86cb\u767d-TEVP-rsTEV-\u6a19\u7684\u86cb\u767d\uff09\uff0c\u7136\u5f8cTEVP\u53ef\u9032\u884c\u5206\u5b50\u5167\u6216\u5206\u5b50\u9593\u5207\u5272\u53cd\u61c9\uff0c\u88fd\u9020\u51fa\u539f\u614b\u86cb\u767d(\u57162B)\u3002<\/p>\n
\u57162\u3001Trans \u8207Cis \u5169\u7a2e\u65b9\u6cd5\u7684\u7c21\u6613\u793a\u610f\u5716\u3002\uff08A\uff09Trans\u65b9\u6cd5\u662f\u5728\u540c\u4e00\u7d30\u80de\u5167\u7522\u751f\u201c\u651c\u5e36\u86cb\u767d-reTEV-\u6a19\u7684\u86cb\u767d\u201d\u8207TEVP\u5169\u500b\u86cb\u767d\uff0cTEVP\u8fa8\u8b58rsTEV\u5e8f\u5217\uff0c\u7531\u53f3\u800c\u5de6\u4f9d\u5e8f\u547d\u540d\u70baP1-P6\u3002Z\u4ee3\u8868\u6a19\u7684\u86cb\u767d\u7684\u7b2c\u4e00\u500b\u6c28\u57fa\u9178\uff0c\u4f4d\u7f6e\u4ee5P1\u2019\u4ee3\u8868\uff0c\u53ef\u753120\u7a2e\u6c28\u57fa\u9178\u4e2d\u4efb\u610f\u9078\u5176\u4e00\u3002TEVP\u5207\u5272\u4f4d\u7f6e\u4f4d\u65bcP1\u8207P1\u2019\u9593\u3002Tyrosine (Y)\u8207Valine (V)\u5169\u500b\u6c28\u57fa\u9178\u7684\u907a\u50b3\u5bc6\u78bc\u5408\u8d77\u4f86\u5373\u662fSnaBI\u9650\u5236\u9176\u6240\u8fa8\u8a8d\u7684DNA\u5e8f\u5217\u3002\uff08B\uff09Cis\u65b9\u6cd5\u662f\u5728\u540c\u4e00\u7d30\u80de\u5167\u7522\u751f\u4e00\u500b\u300c\u651c\u5e36\u86cb\u767d-TEVP-rsTEV-\u6a19\u7684\u86cb\u767d\u300d\u7684\u878d\u5408\u86cb\u767d\uff0c\u7136\u5f8cTEVP\u53ef\u9032\u884c\u5206\u5b50\u5167\u6216\u5206\u5b50\u9593\u53cd\u61c9\uff0c\u5c07\u6a19\u7684\u86cb\u767d\u5207\u51fa\u4f86\u3002P1\u8207P2\u4ee3\u8868\u63a7\u5236\u57fa\u56e0\u8868\u73fe\u7684\u555f\u52d5\u5b50\u3002<\/p>\n
\u4e0a\u8ff0\u5275\u65b0\u7684\u6280\u8853\uff0c\u4e0d\u50c5\u6210\u529f\u6539\u9032\u878d\u5408\u86cb\u767d\u6280\u8853\u7684\u7f3a\u9ede\uff0c\u53c8\u56e0\u4e0d\u9700\u8cfc\u8cb7\u6602\u8cb4\u7684\u86cb\u767d\u8cea\u6c34\u89e3\u9175\u7d20\uff0c\u53ef\u5927\u5e45\u964d\u4f4e\u5176\u6210\u672c\uff0c\u56e0\u6b64\u5c0d\u86cb\u767d\u8cea\u7814\u7a76\u6216\u5de5\u696d\u751f\u7522\u90fd\u6703\u6709\u7528\u3002\u76ee\u524d\u672c\u6280\u8853\u5df2\u7372\u5f97\u672c\u9662\u5be9\u6838\u901a\u904e\uff0c\u7533\u8acb\u5c08\u5229\u4e2d\uff1b\u8ad6\u6587\u4e5f\u65bc\u4eca\u5e74\u4e94\u6708\u767c\u8868\u65bcProtein Science\u671f\u520a\u3002\u503c\u5f97\u6b23\u6170\u7684\u662f\uff0c\u7acb\u523b\u53d7\u5230\u4e0d\u5c11\u570b\u5916\u7814\u7a76\u4eba\u54e1\u7684\u91cd\u8996\uff0c\u4f86\u51fd\u5c0b\u6c42\u5408\u4f5c\u6216\u6280\u8853\u8f49\u79fb\u3002\u77ed\u77ed\u4e00\u500b\u6708\u671f\u9593\uff0c\u5df2\u540c\u610f\u6280\u8853\u79fb\u8f49\u7d66\u4ee5\u8272\u5217\u8036\u8def\u6492\u51b7\u5e02\u7684\u7336\u592a\u5927\u5b78\u3001\u5967\u5730\u5229Paris Lodron\u5927\u5b78\u3001\u745e\u58eb\u8607\u9ece\u4e16\u5927\u5b78\u7b49\u7814\u7a76\u6a5f\u69cb\uff0c\u5c0d\u63d0\u5347\u672c\u9662\u570b\u969b\u77e5\u540d\u5ea6\u5c07\u6703\u6709\u52a9\u76ca\u3002<\/p>\n
\u53c3\u8003\u6587\u737b\uff1a\uff08*corresponding author\uff09<\/p>\n
Yan-Ping Shih, Wen-Mei Kung, Jui-Chuan Chen, Chia-Hui Yeh, Andrew H.-J. Wang and Ting-Fang Wang* (2002) High throughput screening of soluble recombinant proteins. Protein Science 11, 1714-1719.
\nTing-Fang Wang* and Andrew H.-J. Wang (2004) High-throughput screening of soluble recombinant proteins. Chapter 5 of \u201cPurifying Proteins for Proteomics: A Laboratory Manual\u201d edited by Richard J. Simpson, Cold Spring Harbor Laboratory Press, New York.
\nYan-Ping Shih, Hui-Chung Wu, Su-Ming Hu, Ting-Fang Wang* and Andrew H.-J. Wang* (2005) Self-cleavage of fusion protein in vivo using TEV protease to yield native. Protein Science 14, 936-941.
\n\u8a3b\uff1a\u672c\u6587\u7531\u751f\u5316\u6240\u52a9\u7814\u7a76\u54e1\u738b\u5ef7\u65b9\u64b0\u7a3f\uff0c\u7279\u8058\u7814\u7a76\u54e1\u517c\u6240\u9577\u738b\u60e0\u921e\u9662\u58eb\u6821\u7a3f\u3002
\n\u5c08\u696d\u7528\u8a9e\u4e4b\u4e2d\u82f1\u7ffb\u8b6f\uff0c\u8acb\u53c3\u95b1\u738b\u60e0\u921e\u9662\u58eb\u7e3d\u6821\u95b1\u4e4b\u300a\u751f\u5316\u5be6\u9a57\u2014\u57fa\u790e\u64cd\u4f5c\u539f\u7406\u8207\u65b9\u6cd5\u300b\uff082005\u5e74\uff0c\u5049\u660e\u5716\u66f8\u6709\u9650\u516c\u53f8\u51fa\u7248\uff09<\/p>\n","protected":false},"excerpt":{"rendered":"
\u86cb\u767d\u8cea\u662f\u57fa\u56e0\u8868\u73fe\u7684\u7d42\u7522\u7269\uff0c\u751f\u7269\u9ad4\u5167\u9032\u884c\u53cd\u61c9\u591a\u4ee5\u86cb\u767d\u8cea\u70ba\u4e3b\uff0c\u800c\u975eDNA\u6216RNA\uff0c\u6240\u4ee5\u8981\u4e86\u89e3\u751f\u547d\u7684\u5967\u79d8\uff0c\u5fc5\u9808\u8981\u5c0d\u86cb […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[4],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/19183"}],"collection":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/comments?post=19183"}],"version-history":[{"count":1,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/19183\/revisions"}],"predecessor-version":[{"id":19184,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/19183\/revisions\/19184"}],"wp:attachment":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media?parent=19183"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/categories?post=19183"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/tags?post=19183"}],"curies":[{"name":"wp","href":"https:\/api.w.org\/{rel}","templated":true}]}}