{"id":18261,"date":"2019-05-23T00:02:31","date_gmt":"2019-05-22T16:02:31","guid":{"rendered":"https:\/newsletter.sinica.edu.tw\/?p=18261"},"modified":"2021-08-05T08:18:05","modified_gmt":"2021-08-05T00:18:05","slug":"%e3%80%90%e6%9c%ac%e6%9c%9f%e5%b0%88%e6%ac%84%e3%80%91%e6%aa%a2%e6%b8%ac%e7%92%b0%e5%a2%83%e8%88%87%e9%a3%9f%e7%89%a9%e6%b1%a1%e6%9f%93%e6%af%8f%e5%80%8b%e4%ba%ba%e9%83%bd%e5%8f%af%e4%bb%a5%e5%81%9a","status":"publish","type":"post","link":"https:\/newsletter.sinica.edu.tw\/18261\/","title":{"rendered":"\u3010\u5c08\u6b04\u3011\u6aa2\u6e2c\u74b0\u5883\u8207\u98df\u7269\u6c61\u67d3\u6bcf\u500b\u4eba\u90fd\u53ef\u4ee5\u505a\uff1a\u6dfa\u8ac7\u7d30\u83cc\u751f\u7269\u611f\u61c9\u5668"},"content":{"rendered":"
\u74b0\u5883\u4e2d\u6709\u4ec0\u9ebc\u6c61\u67d3\uff1f\u5b83\u662f\u5982\u4f55\u91cf\u6e2c\u7684\u5462\uff1f\u6211\u5011\u6bcf\u65e5\u5fc5\u9808\u98f2\u98df\u7684\u6c34\u3001\u725b\u5976\u3001\u96de\u86cb\u662f\u5426\u542b\u6709\u4e0d\u5b89\u5168\u7684\u6dfb\u52a0\u7269\u6216\u6297\u751f\u7d20\uff1f\u5b83\u5011\u662f\u5982\u4f55\u88ab\u6aa2\u6e2c\u7684\u5462\uff1f\u4e00\u822c\u5e38\u898b\u7684\u505a\u6cd5\u662f\u5c07\u6536\u96c6\u7684\u6a23\u54c1\u9001\u5230\u5be6\u9a57\u5ba4\uff0c\u5229\u7528\u6c23\u76f8\u5c64\u6790-\u8cea\u8b5c\u5100 (GC-MS) \u6216\u9ad8\u6548\u80fd\u6db2\u76f8\u5c64\u6790\u5100(HPLC)\u7b49\u7269\u7406\u5316\u5b78\u65b9\u6cd5\u4f86\u5206\u6790\uff0c\u96d6\u7136\u9019\u4e9b\u65b9\u6cd5\u53ef\u4ee5\u5f97\u5230\u9ad8\u5ea6\u6e96\u78ba\u7684\u7d50\u679c\uff0c\u4f46\u662f\u8f03\u70ba\u6602\u8cb4\u3001\u8017\u6642\uff0c\u4e5f\u4e0d\u80fd\u5728\u73fe\u5834\u5373\u6642\u9032\u884c\u3002\u5fae\u751f\u7269\u751f\u7269\u611f\u61c9\u5668 (microbial biosensor) \u662f\u4e00\u7a2e\u76f8\u5c0d\u7c21\u55ae\u3001\u6210\u672c\u9069\u4e2d\u3001\u651c\u5e36\u65b9\u4fbf\u7684\u6aa2\u6e2c\u5de5\u5177\uff0c\u662f\u4f5c\u70ba\u76e3\u6e2c\u6c61\u67d3\u7269\u66ff\u4ee3\u65b9\u6cd5\u7684\u597d\u9078\u64c7\u3002<\/p>\n
\u751f\u7269\u611f\u61c9\u5668\uff08biosensor, \u5728\u672c\u7bc7\u4e2d\u662f\u6307\u88ab\u5229\u7528\u4f86\u7576\u4f5c\u611f\u6e2c\u5668\u7684\u751f\u7269\u6750\u6599\uff0c\u4e0d\u662f\u7528\u65bc\u611f\u6e2c\u751f\u7269\u5206\u5b50\u7684\u611f\u6e2c\u5668\uff09\u5229\u7528\u751f\u7269\u6750\u6599\u4f86\u8fa8\u8b58\u74b0\u5883\u4e2d\u7684\u76ee\u6a19\u5206\u6790\u7269\uff0c\u63a5\u8457\u5f15\u767c\u4e00\u500b\u5c0d\u61c9\u6027\u7684\u751f\u7269\u53cd\u61c9\uff0c\u7522\u751f\u53ef\u6e2c\u91cf\u7684\u5149\u5b50\u6216\u96fb\u5b50\u8a0a\u865f\uff0c\u900f\u904e\u7269\u7406\u5316\u5b78\u5206\u6790\u5100\u5668\u7d50\u5408\u6578\u64da\u5206\u6790\u8655\u7406\u8edf\u9ad4\u5c07\u8a0a\u865f\u8f49\u70ba\u6613\u8fa8\u8b80\u7684\u6578\u64da\u6216\u5716\u8868\u3002\u5e38\u88ab\u7528\u4f86\u4f5c\u70ba\u751f\u7269\u611f\u61c9\u5668\u7684\u751f\u7269\u6750\u6599\u5305\u62ec\u7d14\u5316\u7684\u9176\u3001\u6297\u9ad4\u3001\u6838\u9178\u3001\u7d30\u80de\u53d7\u9ad4\u6216\u6d3b\u7d30\u80de\u7b49\uff0c\u5404\u6750\u6599\u6709\u5176\u512a\u7f3a\u9ede\uff0c\u76ee\u524d\u9019\u4e9b\u6750\u6599\u88ab\u5ee3\u6cdb\u61c9\u7528\u65bc\u74b0\u5883\u3001\u91ab\u85e5\u6aa2\u6e2c\u88e1\u3002\u5728\u9019\u4e9b\u751f\u7269\u6750\u6599\u4e2d\uff0c\u7d30\u80de\u4f7f\u7528\u6d3b\u7684\u539f\u6838\u7d30\u80de\u6216\u771f\u6838\u7d30\u80de\uff0c\u4e0d\u50c5\u80fd\u53cd\u6620\u76ee\u6a19\u5206\u6790\u7269\u7684\u5b58\u5728\uff0c\u9084\u80fd\u53cd\u6620\u5b83\u5c0d\u751f\u7269\u7684\u6bd2\u6027\uff0c\u63d0\u4f9b\u5f85\u6e2c\u5206\u6790\u7269\u7684\u751f\u7269\u5b89\u5168\u8cc7\u8a0a\u3002\u76f8\u8f03\u65bc\u771f\u6838\u7d30\u80de\uff0c\u7d30\u83cc\u4f5c\u70ba\u751f\u7269\u611f\u61c9\u5668\u7684\u8fa8\u8b58\u6750\u6599\u5728\u64cd\u4f5c\u4e0a\u8f03\u7c21\u6613\u3001\u53cd\u61c9\u6642\u9593\u8f03\u77ed\uff0c\u5bb9\u6613\u4fdd\u5b58\u4ee5\u53ca\u8907\u88fd\uff0c\u800c\u4e14\u5728\u60e1\u52a3\u74b0\u5883\u4e2d\u7684\u751f\u5b58\u8010\u53d7\u529b\u8f03\u9ad8\uff0c\u7d30\u83cc\u4e5f\u56e0\u6b64\u66f4\u5229\u65bc\u6aa2\u6e2c\u5206\u6790\u3002<\/p>\n
\u5929\u7136\u7684\u7d30\u83cc\u548c\u57fa\u56e0\u8f49\u6b96\u7684\u7d30\u83cc\u5df2\u5ee3\u6cdb\u88ab\u7814\u7a76\u4ee5\u61c9\u7528\u65bc\u74b0\u5883\u98a8\u96aa\u7684\u6aa2\u6e2c\uff08\u5716\u4e00a\uff09\u3002\u5929\u7136\u7684\u7d30\u83cc\u80fd\u900f\u904e\u751f\u7406\u6d3b\u6027\u7684\u6539\u8b8a\u53cd\u6620\u5916\u4f86\u523a\u6fc0\u7684\u7a0b\u5ea6\u8207\u7a2e\u985e\uff0c\u5916\u5728\u56e0\u5b50\u80fd\u5f71\u97ff\u7d30\u83cc\u7684\u4ee3\u8b1d\u3001\u57fa\u56e0\u8868\u73fe\u6216\u86cb\u767d\u8cea\u4fee\u98fe\uff0c\u9032\u800c\u5f71\u97ff\u7d30\u83cc\u751f\u7406\u884c\u70ba\u53cd\u61c9\uff0c\u900f\u904e\u7d30\u83cc\u578b\u614b\u3001\u5927\u5c0f\u3001\u9846\u7c92\u6027\u3001\u904b\u52d5\u80fd\u529b\u6216\u751f\u5b58\u529b\u7684\u6539\u8b8a\u53ef\u4ee5\u8a55\u4f30\u74b0\u5883\u7684\u512a\u52a3\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u5929\u751f\u767c\u51b7\u5149\u7684\u7d30\u83cc\uff08Aliivibrio fischeri<\/em>\u3001Photobacterium phosphoreum<\/em>\u7b49\uff09\u4e5f\u662f\u8a55\u4f30\u74b0\u5883\u56e0\u5b50\u7684\u597d\u5de5\u5177\uff0c\u74b0\u5883\u4e2d\u7684\u6bd2\u7269\u53ef\u85c9\u7531\u7834\u58de\u96fb\u5b50\u50b3\u905e\u93c8\u5f71\u97ff\u7d30\u83cc\u7684\u4ee3\u8b1d\uff0c\u964d\u4f4e\u51b7\u5149\u7684\u7522\u751f\uff0c\u914d\u5408\u5149\u5b78\u5100\u5668\u5206\u6790\u5373\u53ef\u8a55\u4f30\u74b0\u5883\u56e0\u5b50\u5c0d\u751f\u7269\u662f\u5426\u6709\u6bd2\u6027\uff0c\u4f8b\u5982\u8cbb\u6c0f\u5f27\u83cc\uff08Aliivibrio fischeri<\/em>\uff09\u5728\u542b\u6709\u591a\u74b0\u6027\u82b3\u9999\u5316\u5408\u7269\u7684\u74b0\u5883\u4e2d\uff0c\u5b83\u7684\u5929\u7136\u51b7\u5149\u88ab\u6291\u5236\u7522\u751f\uff0cA. fischeri<\/em> \u4e5f\u56e0\u6b64\u88ab\u74b0\u4fdd\u7f72\u6307\u5b9a\u70ba\u751f\u7269\u6025\u6bd2\u6027\u7684\u6aa2\u6e2c\u65b9\u6cd5\u4e4b\u4e00 ( \u300c\u751f\u7269\u6025\u6bd2\u6027\u6aa2\u6e2c\u65b9\u6cd5-\u7d30\u83cc\u51b7\u5149\u6cd5\u300d106\u5e746\u670814\u65e5\u74b0\u7f72\u6aa2\u5b57\u7b2c1060043097\u865f\u516c\u544a \u81ea\u4e2d\u83ef\u6c11\u570b106\u5e749\u670815\u65e5\u751f\u6548, NIEA B301.10C\u00a0)\u3002<\/p>\n \u76f8\u8f03\u65bc\u5929\u7136\u7d30\u83cc\uff0c\u57fa\u56e0\u8f49\u6b96\u7684\u7d30\u83cc\u80fd\u7522\u751f\u5c0d\u76ee\u6a19\u5206\u6790\u7269\u6709\u8f03\u5c08\u4e00\u7684\u53cd\u61c9\uff0c\u6aa2\u6e2c\u8a0a\u865f\u4e5f\u5f80\u5f80\u8f03\u70ba\u986f\u8457\u3002\u5c07\u5e36\u6709\u7279\u5b9a\u7684\u555f\u52d5\u5b50(promoter)\u8207\u5831\u544a\u57fa\u56e0(reporter gene)\u7684\u8cea\u9ad4\u8f49\u6b96\u5230\u5929\u7136\u7684\u7d30\u83cc\u88e1\u6216\u63d2\u5165\u7d30\u83cc\u7684\u67d3\u8272\u9ad4\u4e2d\uff0c\u5728\u7279\u5b9a\u7684\u76ee\u6a19\u5206\u6790\u7269\u523a\u6fc0\u4e0b\uff0c\u5c08\u4e00\u7684\u555f\u52d5\u5b50\u6703\u88ab\u6d3b\u5316\uff0c\u5831\u544a\u57fa\u56e0\u4e5f\u56e0\u6b64\u88ab\u95dc\u9589\u6216\u88ab\u8a98\u5c0e\u8868\u73fe\uff08\u5716\u4e00b\uff09\uff0c\u9020\u6210\u5831\u544a\u86cb\u767d\u88ab\u8f49\u8b6f\u7684\u91cf\u6e1b\u5c11\u6216\u589e\u52a0\u3002\u4e00\u822c\u5e38\u898b\u7684\u5831\u544a\u86cb\u767d\u6709\u87a2\u5149\u86cb\u767d \u00a0(fluorescent protein)\u3001\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176 (\u03b2-galactosidase\uff0clacZ<\/em>)\u3001\u7d30\u83cc\u51b7\u5149\u9176 (lux<\/em>)\u3001\u87a2\u706b\u87f2\u51b7\u5149\u9176 (luc<\/em>)\uff0c\u9019\u4e9b\u5831\u544a\u86cb\u767d\u901a\u5e38\u85c9\u7531\u5149\u5b78\u5206\u6790\u5100\u5668\u5373\u53ef\u91cf\u6e2c\u542b\u91cf\u7684\u591a\u5be1\uff0c\u56e0\u6b64\u53ef\u4ee5\u9032\u800c\u5f97\u77e5\u76ee\u6a19\u5206\u6790\u7269\u7684\u7a2e\u985e\u53ca\u542b\u91cf\u3002<\/p>\n