{"id":15485,"date":"2018-11-22T00:04:20","date_gmt":"2018-11-21T16:04:20","guid":{"rendered":"https:\/newsletter.sinica.edu.tw\/?p=15485"},"modified":"2020-12-30T17:14:36","modified_gmt":"2020-12-30T09:14:36","slug":"mbd3%e8%9b%8b%e7%99%bd%e7%9a%84c%e7%ab%af%e5%85%b7%e5%82%99z-dna%e6%93%ac%e6%85%8b%e7%89%b9%e6%80%a7%e4%b8%a6%e5%8f%af%e5%8f%96%e4%bb%a3z-dna%e8%88%87za%e7%b5%90%e5%90%88","status":"publish","type":"post","link":"https:\/newsletter.sinica.edu.tw\/15485\/","title":{"rendered":"MBD3\u86cb\u767d\u7684C\u7aef\u5177\u5099Z-DNA\u64ec\u614b\u7279\u6027\u4e26\u53ef\u53d6\u4ee3Z-DNA\u8207Za\u7d50\u5408"},"content":{"rendered":"<p>\u672c\u9662\u751f\u7269\u5316\u5b78\u7814\u7a76\u6240\u738b\u60e0\u921e\u9662\u58eb\u5be6\u9a57\u5ba4\u767c\u73feMBD3\u86cb\u767d\u7684C\u7aef\u6709\u4e00\u6bb5\u5bcc\u542b\u5929\u9580\u51ac\u80fa\u9178(aspartate)\u548c\u9ea9\u80fa\u9178(glutamate)\u7684\u5340\u57df(D\/E-rich domain)\u80fd\u8207ADAR1\u4e0a\u4e4bZ-DNA\u7d50\u5408\u57df(Z\uf061)\u7d50\u5408\uff0c\u63a8\u8ad6\u9019\u6bb5\u80dc\u80bd\u5177\u5099DNA\u64ec\u614b\u7279\u6027\u3002\u85c9\u7531\u5169\u500b\u86cb\u767d\u8cea\u4e4b\u9593\u76f8\u4e92\u4f5c\u7528\u4f86\u8abf\u63a7B-DNA\u548cZ-DNA\u69cb\u5f62\u4e4b\u9593\u7684\u8f49\u8b8a\uff0c\u9032\u800c\u5f71\u97ff\u57fa\u56e0\u8868\u73fe\u3002<\/p>\n<p>\u5728\u7d30\u80de\u4e2d\u666e\u904d\u5b58\u5728\u7684DNA\u96d9\u80a1\u87ba\u65cb\u662f\u53f3\u65cb\u5f62\u614b\u7684B-DNA\u30021979\u5e74\uff0c\u738b\u60e0\u921e\u9662\u58eb\u5728\u9ebb\u7701\u7406\u5de5\u5b78\u9662Alexander Rich\u6559\u6388\u5be6\u9a57\u5ba4\u7814\u7a76\u6642\u89e3\u958b\u4e86\u4e00\u7a2e\u5168\u65b0\u5177\u5de6\u65cb\u5f62\u614b\u7684DNA\u96d9\u80a1\u87ba\u65cb\u7d50\u69cb\uff0c\u5c07\u5176\u547d\u540d\u70baZ-DNA\u3002Z-DNA\u662f\u9ad8\u80fd\u800c\u4e0d\u7a69\u5b9a\u7684\u7d50\u69cb\u4e14\u504f\u597d\u5728\u6709\u560c\u5464\u548c\u5627\u5576\u4ea4\u66ff\u7684\u5e8f\u5217\u4e2d\u5f62\u6210\uff0c\u4f8b\u5982CpG\u4ea4\u66ff\u91cd\u8907\u5e8f\u5217\u3002\u7576\u7d30\u80de\u9032\u884c\u8f49\u9304(transcription)\u6216\u8005\u67d3\u8272\u8cea\u91cd\u5851\u8907\u5408\u9ad4(chromatin remodeling complex)\u4f5c\u7528\u6642\u6703\u7522\u751f\u8ca0\u8d85\u87ba\u65cb(negative supercoiling)\uff0c\u6b64\u8ca0\u8d85\u87ba\u65cb\u53ef\u63d0\u4f9b\u5c40\u90e8B-DNA\u8f49\u8b8a\u6210Z-DNA\u6240\u9700\u7684\u80fd\u91cf\u3002\u53e6\u5916\uff0cZ\uf061\u80fd\u5920\u8a98\u5c0e\u4e26\u7a69\u5b9aZ-DNA\u7d50\u69cb\u3002\u76ee\u524d\u5c0d\u65bcZ-DNA\u7684\u751f\u7406\u529f\u80fd\u6240\u77e5\u6709\u9650\u3002\u6709\u7814\u7a76\u986f\u793a\u555f\u52d5\u5b50\u4e0aZ-DNA\u7684\u5f62\u6210\u548c\u57fa\u56e0\u8abf\u63a7\u6709\u95dc\u806f\u3002<\/p>\n<p>\u6b64\u6b21\u7814\u7a76\u767c\u73feD\/E-rich domain\u8207Z\uf061\u5177\u6709\u9ad8\u89aa\u548c\u529b\uff0c\u80fd\u5920\u8207Z-DNA\u7af6\u722d\u4e26\u53d6\u800c\u4ee3\u4e4b\u8207Z\uf061\u7d50\u5408\u3002\u53e6\u4e00\u65b9\u9762\uff0cD\/E-rich domain\u6703\u85c9\u7531\u8207MBD3\u7684N\u7aef\u7532\u57fa\u5316CpG\u7d50\u5408\u57df(methyl-CpG-binding domain)\u7d50\u5408\u800c\u4fc3\u4f7fMBD3\u5f62\u6210\u4e8c\u805a\u9ad4\uff0c\u6b64\u4e8c\u805a\u9ad4\u6703\u59a8\u7919D\/E-rich domain\u8207Z\uf061\u7684\u7d50\u5408\u3002\u7531\u6b64\u63a8\u8ad6\u7576MBD3\u548cDNA\u7d50\u5408\u5f8c\u6703\u91cb\u653eD\/E-rich domain\u8207Z-DNA\u7af6\u722d\u4e26\u53d6\u4ee3\u4e4b\u8207Z\uf061\u7d50\u5408\uff0c\u5931\u53bbZ\uf061\u7684Z-DNA\u8b8a\u5f97\u4e0d\u7a69\u5b9a\u800c\u6613\u8f49\u8b8a\u6210B-DNA\u3002\u6b64\u9805\u7814\u7a76\u9996\u6b21\u5448\u73fe\u5177\u6709\u80fd\u529b\u53d6\u4ee3Z-DNA\u8207Z\uf061\u7d50\u5408\u7684D\/E-rich domain\uff0c\u4e26\u70ba\u5c40\u90e8DNA\u69cb\u578b\u7684\u8f49\u8b8a\u63d0\u4f9b\u5275\u65b0\u7684\u8abf\u63a7\u6a5f\u5236\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u7531\u65bc\u4e4b\u524d\u7684\u7814\u7a76\u986f\u793aMBD3\u548cADAR1\u7686\u5728\u8abf\u63a7\u80da\u80ce\u5e79\u7d30\u80de\u7684\u591a\u80fd\u6027\u548c\u5206\u5316\u4e0a\u626e\u6f14\u91cd\u8981\u89d2\u8272\uff0c\u662f\u5426Z-DNA\u7684\u5f62\u6210\u548c\u80da\u80ce\u767c\u80b2\u4e4b\u9593\u5b58\u5728\u95dc\u806f\u6027\uff0c\u5c07\u662f\u672a\u4f86\u4e00\u500b\u91cd\u8981\u7684\u7814\u7a76\u65b9\u5411\u3002<\/p>\n<p>\u672c\u8ad6\u6587\u6a19\u984c\u70ba\u300cThe C-terminal D\/E-rich domain of MBD3 is a putative Z-DNA mimic that competes for Z\uf061 DNA-binding activity \u300d\uff0c\u5df2\u57282018\u5e7410\u670810\u65e5\u767c\u8868\u65bc\u570b\u969b\u671f\u520a\u300c\u6838\u9178\u7814\u7a76\u300d(Nucleic Acids Research)\u3002\u7b2c\u4e00\u4f5c\u8005\u70ba\u674e\u7dba\u83ef\u535a\u58eb\uff0c\u76ee\u524d\u662f\u738b\u60e0\u921e\u9662\u58eb\u5be6\u9a57\u5ba4\u7684\u535a\u58eb\u5f8c\u7814\u7a76\u54e1\u3002<\/p>\n<p>\u8ad6\u6587\u5168\u6587\uff1a <a href=\"https:\/doi.org\/10.1093\/nar\/gky933\">https:\/doi.org\/10.1093\/nar\/gky933<\/a><\/p>\n<p>\uff08\u751f\u5316\u6240\uff09<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u672c\u9662\u751f\u7269\u5316\u5b78\u7814\u7a76\u6240\u738b\u60e0\u921e\u9662\u58eb\u5be6\u9a57\u5ba4\u767c\u73feMBD3\u86cb\u767d\u7684C\u7aef\u6709\u4e00\u6bb5\u5bcc\u542b\u5929\u9580\u51ac\u80fa\u9178(aspartate)\u548c\u9ea9\u80fa\u9178(g [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":15488,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[988,984],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/15485"}],"collection":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/comments?post=15485"}],"version-history":[{"count":2,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/15485\/revisions"}],"predecessor-version":[{"id":23817,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/15485\/revisions\/23817"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media\/15488"}],"wp:attachment":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media?parent=15485"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/categories?post=15485"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/tags?post=15485"}],"curies":[{"name":"wp","href":"https:\/api.w.org\/{rel}","templated":true}]}}