{"id":14368,"date":"2018-08-16T00:00:13","date_gmt":"2018-08-15T16:00:13","guid":{"rendered":"https:\/newsletter.sinica.edu.tw\/?p=14368"},"modified":"2021-08-12T14:42:53","modified_gmt":"2021-08-12T06:42:53","slug":"%e5%be%9e%e3%80%8c%e9%ab%98%e4%b8%ad%e7%94%9f%e3%80%8d%e5%88%b0%e3%80%8c%e5%88%86%e5%ad%90%e7%94%9f%e7%89%a9%e5%ad%b8%e5%ae%b6%e3%80%8d-%e5%b0%88%e8%a8%aa%e8%a9%b9%e4%b8%96%e9%b5%ac","status":"publish","type":"post","link":"https:\/newsletter.sinica.edu.tw\/14368\/","title":{"rendered":"\u5f9e\u300c\u9ad8\u4e2d\u751f\u300d\u5230\u300c\u5206\u5b50\u751f\u7269\u5b78\u5bb6\u300d \u5c08\u8a2a\u8a79\u4e16\u9d6c"},"content":{"rendered":"<div class=\"aio-icon-header\">\n<h1 class=\"aio-icon-title ult-responsive\" data-ultimate-target=\"#Info-box-wrap-7138 .aio-icon-title\" data-responsive-json-new=\"{&quot;font-size&quot;:&quot;&quot;,&quot;line-height&quot;:&quot;&quot;}\"><strong>miRNA \u7814\u7a76\u70ba\u4f55\u91cd\u8981<\/strong><\/h1>\n<\/div>\n<div class=\"aio-icon-description ult-responsive\" data-ultimate-target=\"#Info-box-wrap-7138 .aio-icon-description\" data-responsive-json-new=\"{&quot;font-size&quot;:&quot;&quot;,&quot;line-height&quot;:&quot;&quot;}\">miRNA \u53ef\u8abf\u63a7\u8a31\u591a\u751f\u7269\u7684\u57fa\u56e0\u8868\u73fe\uff0c\u4e26\u5f71\u97ff\u4eba\u985e\u764c\u75c7\u7684\u767c\u751f\u6a5f\u5236\uff0c\u662f\u73fe\u4ee3\u91ab\u7642\u7684\u95dc\u9375\u5206\u5b50\u3002\u8a79\u4e16\u9d6c\u526f\u6559\u6388\u4ee5 miRNA \u70ba\u4e3b\u8981\u7814\u7a76\u9818\u57df\uff0c\u73fe\u8077\u65bc\u81fa\u5927\u91ab\u5b78\u9662\u5fae\u751f\u7269\u5b78\u79d1\u66a8\u7814\u7a76\u6240\uff0c\u66fe\u5728\u4e2d\u7814\u9662\u912d\u6dd1\u73cd\u9662\u58eb\u9580\u4e0b\u6c42\u5b78\uff0c\u4e26\u5c07\u7814\u7a76\u6210\u679c\u767c\u8868\u65bc\u300aScience\u300b\uff0c\u8fc4\u4eca\u6301\u7e8c\u62d3\u5c55 miRNA \u7684\u57fa\u790e\u77e5\u8b58\u3002\u800c\u4ed6\u7684\u7814\u7a76\u751f\u6daf\u8d77\u9ede\uff0c\u662f\u9ad8\u4e2d\u6642\u4f86\u5230\u4e2d\u7814\u9662\u53c3\u8207\u7684\u8f14\u5c0e\u5be6\u9a57\u8a08\u756b\u3002<\/div>\n<div data-ultimate-target=\"#Info-box-wrap-7138 .aio-icon-description\" data-responsive-json-new=\"{&quot;font-size&quot;:&quot;&quot;,&quot;line-height&quot;:&quot;&quot;}\"><\/div>\n<div data-ultimate-target=\"#Info-box-wrap-7138 .aio-icon-description\" data-responsive-json-new=\"{&quot;font-size&quot;:&quot;&quot;,&quot;line-height&quot;:&quot;&quot;}\"><\/div>\n<div data-ultimate-target=\"#Info-box-wrap-7138 .aio-icon-description\" data-responsive-json-new=\"{&quot;font-size&quot;:&quot;&quot;,&quot;line-height&quot;:&quot;&quot;}\">\n<h1 class=\"contains_subtitle_icon\"><strong><b>\u8e0f\u5165\u5be6\u9a57\u5ba4\u7684\u5951\u6a5f<\/b><\/strong><\/h1>\n<p>\u8207\u5be6\u9a57\u5ba4\u4e00\u9580\u4e4b\u9694\u7684\u8fa6\u516c\u5ba4\u4e2d\uff0c\u8a79\u4e16\u9d6c\u6aa2\u67e5\u8457\u5f85\u9001\u6aa2\u7684\u57fa\u56e0\u5e8f\u5217\uff0c\u4e00\u9762\u56de\u61b6 33 \u5e74\u524d\u52a0\u5165\u7b2c\u4e8c\u5c46\u300c<a href=\"http:\/proj1.sinica.edu.tw\/~hispj\/introduction.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u5317\u5340\u9ad8\u4e2d\u751f\u7269\u5b78\u7fd2\u6210\u5c31\u512a\u7570\u5b78\u751f\u8f14\u5c0e\u5be6\u9a57\u8a08\u756b<\/a>\u300d\uff0c\u5f9e\u6700\u57fa\u790e\u7684\u751f\u7269\u5b78\u5be6\u9a57\u96dc\u52d9\u958b\u59cb\uff0c\u8e0f\u4e0a\u4eca\u5929\u5728\u81fa\u5927\u91ab\u5b78\u9662\u7684\u5206\u5b50\u751f\u7269\u5b78\u7814\u7a76\u4e4b\u8def\u3002\u300c\u9ad8\u4e00\u6642\uff0c\u9694\u9031\u65e5\u53bb\u4e2d\u7814\u9662\uff0c\u4e0a\u5348\u807d\u8ab2\u3001\u4e0b\u5348\u505a\u5be6\u9a57\uff0c\u9019\u6a23\u7684\u5047\u65e5\u5f88\u68d2\uff01\u300d\u8a79\u4e16\u9d6c\u56de\u60f3\u3002<\/p>\n<blockquote>\n<h4><strong>\u4f46\u7576\u6642\u96e2\u958b\u5be6\u9a57\u8a08\u756b\u7684\u540c\u5b78\uff0c\u4e0d\u662f\u56e0\u70ba\u6c92\u8208\u8da3\u505a\u7814\u7a76\u6216\u60f3\u8981\u73a9\u6a02\uff0c\u662f\u56e0\u70ba\u4ed6\u5011\u53bb\u6e96\u5099\u8003\u91ab\u5b78\u7cfb\u4e86\u3002<\/strong><\/h4>\n<\/blockquote>\n<p>\u5c11\u5e74\u8a79\u4e16\u9d6c\u53c3\u8207\u7684\u662f\u4eca\u65e5\u4e2d\u7814\u9662\u300c<a href=\"http:\/proj1.sinica.edu.tw\/~hispj\/introduction.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u9ad8\u4e2d\u751f\u547d\u79d1\u5b78\u8cc7\u512a\u751f\u57f9\u80b2\u8a08\u756b<\/a>\u300d\u4e4b\u524d\u8eab\uff0c\u7576\u6642\u7531\u690d\u7269\u6240\u5354\u540c\u52d5\u7269\u6240\u4e3b\u6301\u3002\u7531\u5be6\u9a57\u8a08\u756b\u6539\u7d44\u70ba\u4eca\u65e5\u7684\u898f\u6a21\uff0c\u5171\u6709 80 \u591a\u4f4d\u7814\u7a76\u54e1\u53c3\u8207\u6307\u5c0e\uff0c\u8d85\u904e 2000 \u540d\u9ad8\u4e2d\u751f\u5f97\u4ee5\u63a5\u53d7\u512a\u79c0\u7684\u751f\u7269\u5b78\u5bb6\u7576\u9762\u50b3\u9053\u89e3\u60d1\u3002\u800c 30 \u591a\u5e74\u4f86\uff0c\u80fd\u5920\u5168\u7a0b\u53c3\u8207\u57f9\u80b2\u8a08\u756b\u7684\u9ad8\u4e2d\u751f\u5927\u7d04\u662f\u5341\u5206\u4e4b\u4e00\u3002<\/p>\n<p>\u56e0\u70ba\u53c3\u8207\u8a08\u756b\uff0c\u8a79\u4e16\u9d6c\u5f97\u5230\u8afe\u8c9d\u723e\u734e\u5f97\u4e3b Sanger \u4e4b\u9580\u751f\u2500\u2500\u5468\u5fb7\u6e90\u535a\u58eb\u3001\u6c34\u7a3b\u57fa\u56e0\u5b9a\u5e8f\u8a08\u756b\u5171\u540c\u4e3b\u6301\u4eba\u2500\u2500\u9114\u5b8f\u6f58\u535a\u58eb\u6307\u5c0e\u3002\u5ea6\u904e\u4e09\u5206\u4e4b\u4e00\u4e16\u7d00\uff0c\u4ed6\u9084\u4f9d\u7a00\u8a18\u5f97\u5e2b\u9577\u5011\u7684\u98a8\u683c\uff0c\u4ed6\u7b11\u8457\u5766\u627f\uff1a\u300c\u4e0a\u8ab2\u5167\u5bb9\u7576\u7136\u5df2\u7d93\u5fd8\u4e86\uff0c\u6709\u4e9b\u8001\u5e2b\u4e5f\u50c5\u8a18\u5f97\u9762\u8c8c\u3002\u5370\u8c61\u4e2d\u6709\u4f4d\u8001\u5e2b\u5f88\u8c6a\u9081\u3001\u6709\u6c5f\u6e56\u5473\uff0c\u4f46\u662f\u540d\u5b57\u537b\u907a\u5931\u5728\u8166\u6d77\u88e1\u3002\u300d<\/p>\n<h2><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/team\/13.svg\" alt=\"\u554f\" width=\"45\" \/><b>\u7814\u7a76\u57fa\u790e\u751f\u7269\u5b78\uff0c\u6709\u5f97\u5230\u5bb6\u4eba\u7684\u652f\u6301\u55ce\uff1f<\/b><\/h2>\n<p class=\"start-reply\"><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/talker\/43.svg\" alt=\"\u7b54\" width=\"45\" \/><span class=\"need-reply-icon\">\u6211\u7576\u5e74\u6210\u7e3e\u4e5f\u53ef\u4ee5\u8003\u4e0a\u91ab\u5b78\u7cfb\uff0c\u4f46\u6211\u7528\u8f14\u5c0e\u5be6\u9a57\u8a08\u756b\u7684\u8cc7\u683c\uff0c\u7504\u9078\u9032\u81fa\u5927\u690d\u7269\u7cfb\u3002\u9019\u7b97\u662f\u6bd4\u8f03\u975e\u4e3b\u6d41\u7684\u9078\u64c7\uff0c\u6211\u7238\u5c31\u5728\u6f6e\u5dde\u8001\u5bb6\u8ddf\u9130\u5c45\u57cb\u6028\u6211\uff1a\u300c\u91ab\u751f\u4e0d\u7576\uff0c\u8dd1\u53bb\u8b80\u500b\u7a2e\u82b1\u7a2e\u8349\u7684\u79d1\u7cfb\u3002\u300d\u7576\u5e74\u8f14\u5c0e\u5be6\u9a57\u8a08\u756b\u7b2c\u4e00\u540d\u7684\u540c\u5b78\uff0c\u6700\u5f8c\u7684\u78ba\u662f\u6210\u70ba\u91ab\u5e2b\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<div class=\"wpb_text_column wpb_content_element\">\n<div class=\"wpb_wrapper\">\n<h2><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/team\/13.svg\" alt=\"\u554f\" width=\"45\" \/><b>\u5728\u912d\u6dd1\u73cd\u9662\u58eb\u9580\u4e0b\u5b78\u7fd2\u5c07\u8fd1\u5341\u5e74\uff0c\u5370\u8c61\u6700\u6df1\u523b\u7684\u662f\u4ec0\u9ebc\u4e8b\u60c5\uff1f<\/b><\/h2>\n<p class=\"start-reply\"><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/talker\/43.svg\" alt=\"\u7b54\" width=\"45\" \/><span class=\"need-reply-icon\"><a href=\"http:\/www.imb.sinica.edu.tw\/ch\/research\/faculty\/mbscc.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u912d\u8001\u5e2b<\/a>\u5f88\u56b4\u8b39\uff0c\u4e0d\u63a5\u53d7\u4efb\u4f55\u5be6\u9a57\u6c92\u6709\u63a7\u5236\u7d44\u7684\u5716\uff1b\u6703\u4ed4\u7d30\u78ba\u8a8d\u6bcf\u500b\u5b78\u751f\u7684\u5be6\u9a57\u7d50\u679c\u80fd\u5426\u91cd\u8907\u518d\u73fe\uff0c\u540c\u6642\u8a18\u5f97\u6240\u6709\u5b78\u751f\u5be6\u9a57\u7684\u7d30\u7bc0\u3002\u9019\u7a2e\u6cbb\u5b78\u98a8\u683c\u4e0b\uff0c\u6bcf\u5f35\u5be6\u9a57\u5716\u8868\u90fd\u8981\u91cd\u8907\u64cd\u4f5c\u6539\u9032\uff0c\u7d2f\u7a4d\u76f8\u7576\u7d2e\u5be6\u7684\u6280\u8853\u8207\u7814\u7a76\u6210\u679c\u3002\u6211\u7684\u535a\u8ad6\u53e3\u8a66\u82b1\u4e86\u4e09\u500b\u5c0f\u6642\uff0c\u6108\u8b1b\u807d\u773e\u6108\u591a\uff0c\u5f88\u591a\u5176\u4ed6\u5be6\u9a57\u5ba4\u7684\u5b78\u751f\u4e2d\u9014\u52a0\u5165\u3002<\/span><\/p>\n<p>\u912d\u8001\u5e2b\u5f88\u91cd\u8996\u5b78\u751f\u7684\u6210\u9577\uff0c\u5e0c\u671b\u6211\u9019\u7a2e\u300c\u571f\u535a\u58eb\u300d\u80fd\u51fa\u570b\u78e8\u7df4\u3002\u800c\u4e14\u4e0d\u6703\u56e0\u70ba\u6211\u5011\u5f88\u719f\uff0c\u5c31\u5e6b\u5b78\u751f\u6253\u7406\u5404\u7a2e\u63a8\u85a6\u9580\u8def\uff0c\u5979\u5e0c\u671b\u6211\u5011\u57f9\u990a\u4e00\u5207\u81ea\u7406\u7684\u80fd\u529b\u3002<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element\">\n<div class=\"wpb_wrapper\">\n<h2><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/team\/13.svg\" alt=\"\u554f\" width=\"45\" \/><b>\u662f\u5426\u4e5f\u5728\u81ea\u5df1\u7684\u5be6\u9a57\u5ba4\u4e2d\uff0c\u518d\u73fe\u4e86\u912d\u8001\u5e2b\u7684\u98a8\u683c\uff1f<\/b><\/h2>\n<p class=\"start-reply\"><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/talker\/43.svg\" alt=\"\u7b54\" width=\"45\" \/><span class=\"need-reply-icon\">\u6211\u5f9e\u912d\u8001\u5e2b\u90a3\u908a\u7e7c\u627f\u7684\uff0c\u5927\u6982\u5c31\u662f\u7d93\u5e38\u548c\u5b78\u751f\u8aaa\uff1a\u300c\u5be6\u9a57\uff0c\u6c92\u6709\u4ec0\u9ebc\u5230\u6642\u5019\u518d\u505a\u3002\u300d\u5e73\u5e38\u6211\u5011\u76f8\u8655\u5f88\u8f15\u9b06\u3001\u6c92\u6709\u7981\u5fcc\u3002\u4f46\u662f\u6211\u4e0d\u63a5\u53d7\u5b78\u751f\u5c0d\u5be6\u9a57\u8aaa\uff1a\u300c\u505a\u5230\u9019\u6a23\u5c31\u597d\u4e86\u300d\u3001\u300c\u5230\u6642\u5019\u518d\u505a\u300d\u3001\u300c\u5982\u679c\u8981\u505a\u7684\u8a71\u2026\u300d\u9019\u985e\u4e0d\u8e0f\u5be6\u7684\u8a71\u3002<\/span><\/p>\n<p>\u6211\u5e38\u548c\u4ed6\u5011\u8aaa\uff1a\u300c\u505a\u5be6\u9a57\uff0c\u8981\u5c31\u73fe\u5728\u3002\u6c92\u6709\u4ec0\u9ebc\u5230\u6642\u5019\uff01\u300d<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element\">\n<div class=\"wpb_wrapper\">\n<h2><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/team\/13.svg\" alt=\"\u554f\" width=\"45\" \/><b>\u76f8\u5c0d\u65bc\u5176\u4ed6\u8077\u696d\uff0c\u7814\u7a76\u5de5\u4f5c\u7684\u7279\u9ede\u662f\u4ec0\u9ebc\uff1f<\/b><\/h2>\n<p class=\"start-reply\"><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/talker\/43.svg\" alt=\"\u7b54\" width=\"45\" \/><span class=\"need-reply-icon\">\u505a\u7814\u7a76\u7684\u597d\u8655\uff0c\u5c31\u662f\u6642\u9593\u53ef\u4ee5\u81ea\u7406\u3002\u6709\u81ea\u7531\u8207\u8cc7\u6e90\u53ef\u4ee5\u53bb\u63a2\u7d22\u81ea\u5df1\u597d\u5947\u7684\u4e8b\u60c5\uff0c\u9019\u6a23\u975e\u5e38\u68d2\u3002\u7576\u7136\uff0c\u6bcf\u500b\u4eba\u7684\u5c6c\u6027\u4e0d\u540c\uff0c\u6709\u4e9b\u4eba\u7684\u6027\u683c\u9069\u5408\u5750\u4e0b\u4f86\u8ffd\u6839\u7a76\u67e2\uff1b\u6709\u4e9b\u4eba\u6bd4\u8f03\u5750\u4e0d\u4f4f\uff0c\u5c31\u672a\u5fc5\u80fd\u4eab\u53d7\u7814\u7a76\u5de5\u4f5c\u3002\u50cf\u6211\u662f\u6bcf\u5929\u5728\u5be6\u9a57\u5ba4\u8dd1\u81a0\u4e5f\u5341\u5206\u5feb\u6a02\u7684\u90a3\u7a2e\u4eba\u3002\u2028<\/span><\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element\">\n<div class=\"wpb_wrapper\">\n<h2><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/team\/13.svg\" alt=\"\u554f\" width=\"45\" \/><b>\u5c0d\u6709\u5fd7\u65bc\u6295\u5165\u751f\u7269\u5b78\u7814\u7a76\u7684\u5b78\u751f\uff0c\u6709\u4ec0\u9ebc\u5efa\u8b70\uff1f<\/b><\/h2>\n<p class=\"start-reply\"><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/talker\/43.svg\" alt=\"\u7b54\" width=\"45\" \/><span class=\"need-reply-icon\">\u751f\u7269\u5b78\u5de5\u4f5c\u7576\u7136\u4e5f\u6709\u73fe\u5be6\u9762\u7684\u58d3\u529b\u3002\u6211\u9019\u500b\u4e16\u4ee3\u548c\u8077\u4f4d\u7684\u5b78\u8005\uff0c\u4e5f\u5e38\u6703\u88ab\u4e0a\u7d1a\u6216\u9577\u8f29\u8a8d\u70ba\u300c\u9084\u4e0d\u5920\u52aa\u529b\u300d\u6216\u300c\u4e0d\u5920\u8070\u660e\u300d\uff0c\u8ad6\u6587\u767c\u8868\u6578\u6216\u7814\u7a76\u6210\u679c\u4e0d\u76e1\u7406\u60f3\u3002\u4f46\u662f\u771f\u7684\u60f3\u6c42\u77e5\u3001\u60f3\u6eff\u8db3\u597d\u5947\u5fc3\u7684\u5b78\u751f\uff0c\u6211\u60f3\u4ed6\u5011\u4e0d\u9700\u8981\u5c0b\u6c42\u5efa\u8b70\uff0c\u5c31\u8d95\u5feb\u958b\u59cb\u52d5\u624b\uff0c\u99ac\u4e0a\u8a2d\u6cd5\u505a\u5be6\u9a57\u5427\uff01<\/span><\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element\">\n<div class=\"wpb_wrapper\">\n<h2><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/team\/13.svg\" alt=\"\u554f\" width=\"45\" \/><b>\u7814\u7a76\u57fa\u790e\u79d1\u5b78\uff0c\u8a2d\u5b9a\u7814\u7a76\u554f\u984c\u6642\u662f\u5426\u8a72\u56de\u61c9\u7279\u5b9a\u7684\u300c\u793e\u6703\u9700\u6c42\u300d\u5462\uff1f<\/b><\/h2>\n<p class=\"start-reply\"><img decoding=\"async\" src=\"http:\/research.sinica.edu.tw\/img\/talker\/43.svg\" alt=\"\u7b54\" width=\"45\" \/><span class=\"need-reply-icon\">\u554a\uff0c\u975e\u5e38\u96e3\u56de\u7b54\u7684\u554f\u984c\u3002(\u5f9e\u5730\u677f\u6383\u8996\u5230\u5929\u82b1\u677f\uff0c\u82e6\u601d)<\/span><\/p>\n<p>\u5f9e\u6b77\u53f2\u7684\u89d2\u5ea6\u770b\uff0c\u751f\u7269\u5b78\u7684\u8d77\u9ede\u5176\u5be6\u662f\u4f9d\u9644\u5728\u5e1d\u570b\u4e3b\u7fa9\u8207\u6b96\u6c11\u6d3b\u52d5\u4e4b\u4e0b\u7684\u535a\u7269\u5b78\u5de5\u4f5c\u3002\u7576\u6642\u7684\u751f\u7269\u5b78\u5bb6\u76ee\u7684\u5f88\u660e\u78ba\uff0c\u5c31\u662f\u70ba\u6b50\u6d32\u5f37\u6b0a\u641c\u96c6\u6d77\u5916\u73cd\u79bd\u7570\u7378\u3001\u5947\u82b1\u602a\u6728\u3001\u751a\u81f3\u662f\u7570\u570b\u4eba\u985e\uff0c\u4f5c\u70ba\u5c55\u793a\u6216\u5a1b\u6a02\u4e4b\u7528\u3002<\/p>\n<p>\u73fe\u4ee3\u57fa\u790e\u751f\u7269\u5b78\u76f8\u5c0d\u4ee5\u300c\u6c42\u77e5\u300d\u70ba\u5c0e\u5411\uff0c\u4e0d\u50cf\u904e\u5f80\u70ba\u4e86\u5e1d\u570b\u8cb4\u65cf\u6216\u6b96\u6c11\u5546\u4eba\u7684\u559c\u597d\u800c\u505a\u3002\u9019\u6a23\u770b\u4f86\uff0c\u6211\u6703\u8aaa\u4e0d\u5fc5\u70ba\u4e86\u4efb\u4f55\u4eba\u7684\u76ee\u7684\uff0c\u800c\u7d14\u70ba\u6c42\u77e5\u800c\u5047\u8a2d\u7814\u7a76\u984c\u76ee\u6bd4\u8f03\u597d\u3002<\/p>\n<p>\u4e0d\u904e\u6211\u5728\u570b\u7acb\u5927\u5b78\u5de5\u4f5c\uff0c\u800c\u4e14\u570b\u5167\u5927\u591a\u6578\u7814\u7a76\u7d93\u8cbb\u4f86\u6e90\u90fd\u4f86\u81ea\u653f\u5e9c\uff0c\u78ba\u5be6\u662f\u5960\u57fa\u5728\u516c\u5171\u8cc7\u6e90\u4e0a\u9032\u884c\u7814\u7a76\u3002\u7576\u7136\u5fc5\u9808\u8981\u8003\u616e\u300c\u56de\u994b\u793e\u6703\u300d\uff0c\u4f46\u54ea\u4e00\u7fa4\u4eba\u7684\u9700\u6c42\u53ef\u4ee5\u4ee3\u8868\u300c\u793e\u6703\u9700\u6c42\u300d\uff1f\u6216\u662f\u8aaa\u751f\u7269\u5b78\u5bb6\u61c9\u8a72\u512a\u5148\u5728\u610f\u8ab0\u7684\u9700\u6c42\u5462\uff1f\u9019\u662f\u6bcf\u500b\u5b78\u8005\u81ea\u5df1\u5fc5\u9808\u62ff\u634f\u7684\u3002<\/p>\n<p>\u5c0d\u6211\u4f86\u8aaa\uff0c\u5728\u57fa\u790e\u79d1\u5b78\u9818\u57df\uff0c\u4ee5\u81ea\u5df1\u7684\u77e5\u8b58\u8207\u597d\u5947\u5fc3\u51fa\u767c\uff0c\u9032\u884c\u5be6\u9a57\u8a2d\u8a08\u5c31\u597d\u3002\u4f46\u662f\u57fa\u790e\u79d1\u5b78\u7814\u7a76\u8005\u5728\u6311\u9078\u4e3b\u984c\u3001\u9032\u884c\u5be6\u9a57\u6642\uff0c\u5fc3\u4e2d\u62b1\u6301\u8457\u300c\u4ee5\u7814\u7a76\u6210\u679c\u56de\u994b\u793e\u6703\u300d\u7684\u671f\u5f85\u8207\u4fe1\u5ff5\u662f\u975e\u5e38\u91cd\u8981\u3001\u800c\u4e14\u5fc5\u8981\u7684\u3002\u8981\u653e\u5728\u5fc3\u88e1\u7684\u662f\uff0c\u9055\u80cc\u79d1\u5b78\u502b\u7406\u3001\u751a\u81f3\u865b\u5047\u7684\u5be6\u9a57\u5c31\u9060\u4f4e\u65bc\u9019\u500b\u57fa\u672c\u8981\u6c42\u3002<\/p>\n<div id=\"attachment_14369\" style=\"width: 838px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14369\" class=\" wp-image-14369\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-01.jpg\" alt=\"\" width=\"828\" height=\"552\" srcset=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-01.jpg 3000w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-01-300x200.jpg 300w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-01-768x512.jpg 768w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-01-1024x683.jpg 1024w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-01-1080x720.jpg 1080w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-01-180x120.jpg 180w\" sizes=\"(max-width: 828px) 100vw, 828px\" \/><p id=\"caption-attachment-14369\" class=\"wp-caption-text\">\u8a79\u4e16\u9d6c\u8a8d\u70ba\uff1a\u7814\u7a76\u9019\u4e9b\u5c0f\u5206\u5b50\u7684\u4f5c\u7528\u6a5f\u5236\uff0c\u9664\uf9ba\u6eff\u8db3\u7d14\u5b78\u8853\u597d\u5947\u5fc3\uff0c\u66f4\u662f\u5c07 miRNA \u8207 siRNA \u61c9\u7528\u5728\u6cbb\u7642\u4eba\u985e\u75be\u75c5\u7684\u91cd\u8981\u57fa\u790e\u3002<br \/>\u651d\u5f71\u2502\u5f35\u8a9e\u8fb0<\/p><\/div>\n<h1 class=\"contains_subtitle_icon\"><strong><b>\u8abf\u63a7\u57fa\u56e0\u8868\u73fe\u7684 miRNA<\/b><\/strong><\/h1>\n<p>\u4eba\u985e\u9ad4\u5167\u6709\u4e0a\u5343\u500b miRNA \u5df2\u5b8c\u6210\u5b9a\u5e8f\uff0c\u5b83\u5011\u53ef\u8abf\u63a7 60% \u7684\u4eba\uf9d0\u57fa\u56e0\u8868\u73fe\u3002\u800c\u8a79\u4e16\u9d6c<a href=\"https:\/spchanlab.wordpress.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u5be6\u9a57\u5ba4<\/a>\u7684\u4e3b\u8981\u7814\u7a76\u9818\u57df\u662f\u5fae\u6838\u7cd6\u6838\u9178\u7684\u57fa\u56e0\u8abf\u63a7\u6a5f\u5236 (microRNA-mediated gene regulation) \u4ee5\u53ca\u5c0f\u5e72\u64fe\u6838\u91a3\u6838\u9178 (small interfering RNA) \u751f\u6210\u8207\u4f5c\u7528\u6a5f\u5236\u7684\u57fa\u790e\u7814\u7a76\u3002<\/p>\n<p>\u8a79\u4e16\u9d6c\u5728\u78a9\u3001\u535a\u58eb\u73ed\u6642\u671f\uff0c\u65bc\u4e2d\u7814\u9662<a href=\"http:\/www.imb.sinica.edu.tw\/ch\/research\/faculty\/mbscc.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u912d\u6dd1\u73cd<\/a>\u9662\u58eb\u9580\u4e0b\u7814\u7a76\u8a0a\u606f\u6838\u91a3\u6838\u9178\u526a\u63a5 (pre-mRNA splicing) \u53cd\u61c9\u4e2d\u7684\u91cd\u8981\u86cb\u767d\u8907\u5408\u9ad4 PRP19-complex\uff0c\u6210\u679c\u65bc 2003 \u5e74\u767c\u8868\u65bc\u300aScience\u300b\uff0c\u81f3\u4eca\u5b78\u754c\u7684\u6700\u65b0\u7814\u7a76\u4ecd\u5f15\u7528\u70ba\u7406\u8ad6\u57fa\u790e\u3002\u4ed6\u96a8\u5f8c\u52a0\u5165\u8036\u9b6f\u5927\u5b78\u00a0<a href=\"https:\/www.bidmc.org\/research\/research-by-department\/pathology\/laboratories\/frank-slack-lab\" target=\"_blank\" rel=\"noopener noreferrer\">Frank Slack<\/a>\u00a0\u535a\u58eb\u7684\u5718\u968a\u9032\ufa08\u535a\u58eb\u5f8c\u7814\u7a76\u3002Slack \u662f\u767c\u73fe let-7 miRNA \u7684\u91cd\u8981\u7814\u7a76\u5718\u968a\u6210\u54e1\uff0c\u4ed6\u7684\u7814\u7a76\u4e5f\u8b49\u5be6 let-7 \u53ef\u8abf\u63a7\u81f4\u764c\u57fa\u56e0 RAS\uff0c\u4e26\u767c\u73fe let-7 \u7684\u8868\u73fe\u8207\u80ba\u764c\u6709\u9ad8\u5ea6\u76f8\u95dc\u6027\u3002<\/p>\n<p>\u5230\u4e86\u535a\u58eb\u5f8c\u6642\u671f\u7684\u7814\u7a76\u4e3b\u984c\uff0c\u8a79\u4e16\u9d6c\u5247\u63a2\u8a0e\u5fae\u6838\u91a3\u6838\u9178 (miRNA) \u8abf\u63a7\u7dda\u87f2\u57fa\u56e0\u8868\u73fe\u7684\u6a5f\u5236\u3002miRNA \u7684\u529f\u80fd\u662f\u8fd1\u5341\u591a\u5e74\u4f86\u7684\u91cd\u8981\u751f\u547d\u79d1\u5b78\u767c\u73fe\uff0c\u5b83\u5011\u7d93\u7531\u6838\u91a3\u6838\u9178\u5e8f\uf99c\u7684\u4e92\u88dc\u6027\uff0c\u8fa8\u8a8d\u4e26\u7d50\u5408\u6a19\u7684\u8a0a\u606f\u6838\u91a3\u6838\u9178 (mRNA)\uff0c\u6291\u5236\u5176\u8a0a\u606f\u7684\u8f49\u8b6f\u529f\u80fd\u3001\u4e26\u4fc3\u4f7f\u5176\u964d\u89e3\u3002<\/p>\n<div id=\"attachment_14370\" style=\"width: 825px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14370\" class=\" wp-image-14370\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1.jpg\" alt=\"\" width=\"815\" height=\"490\" srcset=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1.jpg 4167w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1-300x180.jpg 300w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1-768x462.jpg 768w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1-1024x616.jpg 1024w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1-1080x649.jpg 1080w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1-1280x768.jpg 1280w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1-627x376.jpg 627w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-02-1-440x264.jpg 440w\" sizes=\"(max-width: 815px) 100vw, 815px\" \/><p id=\"caption-attachment-14370\" class=\"wp-caption-text\">miRNA \u5f77\u5f7f\u5728\u5e6b mRNA \u8e29\u524e\u8eca\uff0c\u66ab\u505c\u5f8c\u7e8c\u8f49\u8b6f\u86cb\u767d\u8cea\uff0c\u85c9\u6b64\u8abf\u63a7\u57fa\u56e0\u8868\u73fe\u3002<br \/>\u5716\u8aaa\u8a2d\u8a08\u2502\u5f35\u8a9e\u8fb0<\/p><\/div>\n<p>&nbsp;<\/p>\n<\/div>\n<p>RNA (\u6838\u7cd6\u6838\u9178)\u5728\u751f\u7269\u9ad4\u5167\u4e0d\u50c5\u662f\u57fa\u56e0\u50b3\u8a0a\u8005\uff0c\u66f4\u4ee5\u5176\u4ed6\u5f62\u5f0f\u8abf\u7bc0\u8457\u57fa\u56e0\u8868\u73fe\uff0c\u626e\u6f14\u500b\u9ad4\u767c\u80b2\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u7d30\u80de\u5206\u5316\u7b49\u6a5f\u5236\u7684\u95dc\u9375\u89d2\u8272\u3002<\/p>\n<p>\u63d0\u5230 RNA \uff0c\u591a\u6578\u4eba\u6703\u76f4\u89ba\u60f3\u5230\u5206\u5b50\u751f\u7269\u5b78\u7684<a href=\"https:\/en.wikipedia.org\/wiki\/Central_dogma_of_molecular_biology\" target=\"_blank\" rel=\"noopener noreferrer\">\u4e2d\u5fc3\u539f\u5247<\/a>\uff0c\u5728\u57fa\u56e0\u8868\u73fe\u904e\u7a0b\u4e2d\uff0cmRNA (\u4fe1\u4f7f\u6838\u7cd6\u6838\u9178)\u64d4\u7db1\u8a0a\u606f\u50b3\u905e\u529f\u80fd\uff0c\u4e5f\u53ef\u8aaa\u662f\u593e\u5728 DNA \u548c\u86cb\u767d\u8cea\u4e4b\u9593\u7684\u50b3\u8a0a\u8005\u3002\u7576 DNA \u5e8f\u5217\u8f49\u9304 (transcription) \u6210 mRNA \u4e4b\u5f8c\uff0cmRNA \u6703\u518d\u64d4\u4efb\u6a21\u677f\u3001\u8f49\u8b6f (translation) \u51fa\u86cb\u767d\u8cea\uff0c\u9032\u800c\u5c55\u73fe\u751f\u7269\u5316\u5b78\u5c64\u9762\u7684\u529f\u80fd\uff0c\u800c tRNA\u3001rRNA \u5247\u8f14\u52a9\u6b64\u6a5f\u5236\u904b\u4f5c\u3002<\/p>\n<p>\u6b64\u5916\uff0cmiRNA (\u5fae\u6838\u7cd6\u6838\u9178) \u8207 siRNA (\u5c0f\u5e72\u64fe\u6838\u7cd6\u6838\u9178)\u5ee3\u6cdb\u5b58\u5728\u65bc\u7d30\u83cc\u3001\u690d\u7269\u3001\u52d5\u7269\u9ad4\u4e2d\uff0c\u5b83\u5011\u7684\u529f\u80fd\u4e0d\u540c\u65bc mRNA \uff0c\u81ea\u8eab\u6c92\u6709\u6700\u7d42\u7684\u86cb\u767d\u8cea\u7522\u7269\uff0c\u537b\u80fd\u6c7a\u5b9a\u5176\u4ed6\u57fa\u56e0\u7684\u8868\u73fe\u3002<\/p>\n<blockquote>\n<h4><strong>\u7814\u7a76\u8b49\u5be6 miRNA \u7684\u529f\u80fd\u8207\u4eba\u985e\u764c\u75c7\u3001\u500b\u9ad4\u767c\u80b2\u95dc\u4fc2\u7dca\u5bc6\u3002\u4f46\u662f\uff0cmiRNA \u8abf\u7bc0\u57fa\u56e0\u8868\u73fe\u7684\u65b9\u5f0f\uff0c\u4ecd\u662f\u4e00\u9762\u7a7a\u767d\u904d\u5e03\u7684\u8907\u96dc\u62fc\u5716\u3002<\/strong><\/h4>\n<p>&nbsp;<\/p><\/blockquote>\n<h1 class=\"contains_subtitle_icon\"><strong><b>\u85c9\u7531\u300c\u7dda\u87f2\u300d\u4e86\u89e3 miRNA<\/b><\/strong><\/h1>\n<p>miRNA \u662f\u7dda\u87f2\u5404\u767c\u80b2\u968e\u6bb5\u7684\u4e3b\u8981\u8abf\u63a7\u5206\u5b50\uff0c\u5de7\u5999\u8cab\u4e32\u6574\u500b\u751f\u547d\u53f2\uff1b\u540c\u6642\u4e5f\u56e0\u70ba miRNA \u7684\u57fa\u56e0\u4fdd\u5b88\u6027\uff0c\u5728\u5176\u4ed6\u751f\u7269\u8207\u4eba\u985e\u9ad4\u5167\u6709\u8a31\u591a\u57fa\u56e0\u662f\u4ee5\u76f8\u540c\u6a5f\u5236\u8abf\u63a7\uff0c\u56e0\u6b64\u79d1\u5b78\u5bb6\u6703\u7814\u7a76\u7dda\u87f2\u4f86\u4e86\u89e3 miRNA \u7684\u5967\u79d8\u3002<\/p>\n<p>\u53f2\u4e0a\u7b2c\u4e00\u500b\u88ab\u767c\u73fe\u7684\u5fae\u6838\u91a3\u6838\u9178\u662f\u7dda\u87f2\u7684 lin-4 miRNA \uff0c\u4f86\u81ea\u7dda\u87f2\u767c\u80b2\u65e9\u671f\u88ab\u6d3b\u5316\u7684 lin-4 \u57fa\u56e0\uff0c\u8f49\u9304\u51fa\u7684 RNA \u50c5\u6709 22 \u500b\u6838\u82f7\u9178\uff1blin-4 miRNA \u672c\u8eab\u7121\u6cd5\u8f49\u8b6f\u7522\u751f\u86cb\u767d\u8cea\uff0c\u537b\u80fd\u5f71\u97ff\u53e6\u4e00\u57fa\u56e0 lin-14 \u7684\u8868\u73fe\u3002lin-4 \u4e0d\u6291\u5236 lin-14 \u57fa\u56e0\u7684\u8f49\u9304\u529f\u80fd\uff0c\u537b\u80fd\u58d3\u6291\u4e0b\u4e00\u6b65\u7684\u8f49\u8b6f\u968e\u6bb5\uff0c\u4f7f lin-14 \u7684\u86cb\u767d\u7522\u7269\u5927\u5e45\u6e1b\u5c11\u3002\u6b64\u6a5f\u5236\u5728 1993 \u5e74\u958b\u59cb\u63ed\u9732\uff0c\u7576\u6642\u88ab\u8996\u70ba\u7dda\u87f2\u7279\u6709\u7684\u767c\u80b2\u8abf\u7bc0\u6a5f\u5236\uff0c\u4e26\u672a\u5f15\u8d77\u5ee3\u5927\u6ce8\u610f\u3002<\/p>\n<p>\u76f4\u5230 2000 \u5e74\uff0c<a href=\"https:\/zh.wikipedia.org\/wiki\/%E5%8A%A0%E9%87%8C%C2%B7%E9%B2%81%E5%A4%AB%E6%98%86\" target=\"_blank\" rel=\"noopener noreferrer\">Gary Ruvkun<\/a>\u00a0\u7684\u7814\u7a76\u5718\u968a\u767c\u73fe lin-14 \u53ef\u4ee5\u8abf\u63a7\u4e0b\u6e38\u57fa\u56e0 let-7 \u7684\u8868\u73fe\uff0c\u800c\u7dda\u87f2\u665a\u671f\u767c\u80b2\u6642\u88ab\u6d3b\u5316\u7684 let-7 \u7522\u751f\u7684 miRNA \u53ef\u4ee5\u6291\u5236\u4e0b\u6e38\u57fa\u56e0 lin-41 \u4ee5\u53ca\u6210\u87f2\u767c\u80b2\u57fa\u56e0 lin-29\u3002\u4ee5 miRNA \u8cab\u7a7f\u7dda\u87f2\u751f\u547d\u53f2\u7684\u57fa\u56e0\u8abf\u63a7\u6a5f\u5236\u958b\u59cb\u5f97\u5230\u77da\u76ee\u3002<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div data-ultimate-target=\"#Info-box-wrap-7138 .aio-icon-description\" data-responsive-json-new=\"{&quot;font-size&quot;:&quot;&quot;,&quot;line-height&quot;:&quot;&quot;}\">\n<div id=\"attachment_14371\" style=\"width: 819px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14371\" class=\" wp-image-14371\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-03-1-1024x537.jpg\" alt=\"\" width=\"809\" height=\"424\" srcset=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-03-1-1024x537.jpg 1024w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-03-1-1024x537-300x157.jpg 300w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-03-1-1024x537-768x403.jpg 768w\" sizes=\"(max-width: 809px) 100vw, 809px\" \/><p id=\"caption-attachment-14371\" class=\"wp-caption-text\">miRNAs lin-4 \u548c let-7 \u8abf\u63a7\u6a19\u9776\u57fa\u56e0\u7684\u6642\u9593\u95dc\u4fc2\uff0c\u8cab\u7a7f\u7dda\u87f2\u7684\u5e74\u5e7c\u6642\u671f\u5230\u6210\u87f2\u3002<br \/>\u8cc7\u6599\u4f86\u6e90\u2502<em>Vella, M.C., &amp; Slack, F.J. (2005). C. elegans microRNAs. WormBook : the online review of C. elegans biology, 1-9.<\/em><\/p><\/div>\n<\/div>\n<p>&nbsp;<\/p>\n<p>\u53e6\u5916\uff0c\u65bc 2000 \u5e74\uff0c<a href=\"http:\/labs.biology.ucsd.edu\/pasquinelli\/index.html\" target=\"_blank\" rel=\"noopener noreferrer\">Pasquinelli<\/a>\u00a0\u7684\u7814\u7a76\u5718\u968a\u4e5f\u6307\u51fa let-7 RNA \u5177\u6709\u7269\u7a2e\u9593\u7684\u57fa\u56e0\u4fdd\u5b88\u6027 (<a href=\"https:\/doi.org\/10.1038\/35040556\" target=\"_blank\" rel=\"noopener noreferrer\">Gene conservation<\/a>)\uff0c\u7531\u65bc\u4eba\u985e\u9ad4\u5167\u4e5f\u542b\u6709 miRNA\uff0cmiRNA \u958b\u59cb\u5728\u767c\u80b2\u5b78\u7814\u7a76\u6fc0\u8d77\u6ce2\u703e\u3002<\/p>\n<p>\u6578\u5e74\u5167\uff0clet-7 \u57fa\u56e0\u88ab\u767c\u73fe\u8207\u4eba\u985e\u764c\u75c7\u95dc\u4fc2\u7dca\u5bc6\uff0c\u4f8b\u5982\uff0c\u8207\u80ba\u764c\u75c5\u4eba\u8853\u5f8c\u5b58\u6d3b\u7387\u6709\u76f4\u63a5\u95dc\u4fc2\u3001\u53ef\u4ee5\u76f4\u63a5\u8abf\u63a7\u4eba\u985e\u764c\u75c7\u57fa\u56e0 RAS\u3002\u6b64\u5f8c\uff0c\u8a31\u591a\u7814\u7a76\u5718\u968a\u958b\u59cb\u6295\u5165 miRNA \u7814\u7a76\uff1a\u5305\u542b\u5c0b\u627e\u65b0\u7a2e miRNA\uff0c\u9810\u6e2c\u8207\u9451\u5b9a\u5176\u8abf\u63a7\u76ee\u6a19\u57fa\u56e0\uff1b\u7814\u7a76 miRNA \u5728\u764c\u75c7\u4e2d\u7684\u89d2\u8272\uff0c\u4e26\u6e2c\u8a66\u76f8\u95dc\u7642\u6cd5\u3002miRNA \u6210\u70ba\u5206\u5b50\u751f\u7269\u5b78\u8207\u57fa\u790e\u91ab\u5b78\u9818\u57df\u7684\u8981\u89d2\u3002<\/p>\n<blockquote>\n<h4><strong>\u5404\u7a2e miRNA \u7d50\u5408\u7684\u8f14\u52a9\u86cb\u767d\u5206\u5b50\u5404\u7570\uff0c \u8abf\u63a7\u57fa\u56e0\u8868\u73fe\u7684\u6a5f\u5236\u4e5f\u5145\u6eff\u8907\u96dc\u53ef\u80fd\u6027\u3002<\/strong><\/h4>\n<\/blockquote>\n<p>miRNA \u5982\u4f55\u6291\u5236\u57fa\u56e0\u8868\u73fe\u4ecd\u5b58\u6709\u722d\uf941\uff0c\u67d0\u4e9b\u7814\u7a76\u652f\u6301 miRNA \u6291\u5236\u8f49\u8b6f\u4f5c\u7528\u7684\u8d77\u59cb (initiation)\uff1b\u540c\u6642\u4e5f\u6709\u5b78\u8005\u767c\u73fe\uff0cmiRNA \u6291\u5236\u7684\u662f\u6b63\u5728\u8f49\u8b6f\u7684\u6838\ufa03\u9ad4 (post-initiation repression)\u3002<\/p>\n<h1 class=\"contains_subtitle_icon\"><strong><b>miRNA \u7684\u4f5c\u7528\u9014\u5f91<\/b><\/strong><\/h1>\n<div id=\"attachment_14372\" style=\"width: 697px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14372\" class=\" wp-image-14372\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-04-1.jpg\" alt=\"\" width=\"687\" height=\"860\" srcset=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-04-1.jpg 3000w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-04-1-240x300.jpg 240w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-04-1-768x961.jpg 768w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-04-1-818x1024.jpg 818w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2018\/08\/shihpengchan-mirna-04-1-1080x1351.jpg 1080w\" sizes=\"(max-width: 687px) 100vw, 687px\" \/><p id=\"caption-attachment-14372\" class=\"wp-caption-text\">\u3010miRNA \u548c siRNA \u7684\u751f\u6210\u8207\u4f5c\u7528\u3011<br \/>\u4e00\u822c\u8def\u5f91 (\u5716\u7247\u7531\u4e0a\u81f3\u4e0b)\uff1a<br \/>1. DNA \u8207 RNA \u805a\u5408\u9176\u8f49\u9304\u51fa\u4e0a\u767e\u81f3\u4e0a\u5343\u6838\u82f7\u9178\u9577\u7684 Pri-miRNA\uff0c\u900f\u904e Drosha \u5207\u5272\u70ba 60~70 \u6838\u82f7\u9178\u9577\u7684 pre-miRNA\u3002<br \/>2. pre-miRNA \u7d93\u904e\u8f38\u9001\u86cb\u767d Exportin-5 \u9032\u5165\u7d30\u80de\u8cea\uff0c\u7531 Dicer \u5207\u5272\u70ba\u7d04 22 \u6838\u82f7\u9178\u9577\u5ea6\u7684 miRNA \u548c\u4e92\u88dc\u80a1 miRNA* (\u5c07\u88ab\u79fb\u9664\u964d\u89e3\u7684\u4e00\u80a1)\u3002<br \/>3. miRNA \u548c AGO \u86cb\u767d\u7d50\u5408\u6210 RISC \uff0c\u5c0b\u627e\u4e26\u7d50\u5408\u76ee\u6a19 mRNA\u3002\u5b8c\u7f8e\u7d50\u5408\u5c31\u53ef\u4ee5\u8fc5\u901f\u5207\u65b7\u3001\u964d\u89e3 mRNA\u3002\u7d50\u5408\u4e0d\u5b8c\u5168\uff0c\u5f62\u6210\u7a81\u8d77 (bulge) \u7684\u8a71\uff0c\u5c31\u6703\u6291\u5236\u6838\u7cd6\u9ad4\u8f49\u9304\u529f\u80fd\uff0c\u9032\u800c\u7531\u4f86\u81ea RISC \u7684\u53bb\u817a\u5627\u5576\u9176 (deadenylase) \u8907\u5408\u7269\u964d\u89e3 mRNA\u3002<br \/>\u2014\u2013<br \/>\u8f03\u5c11\u898b\u7684 Mirtron \u8def\u5f91 (\u5716\u7247\u53f3\u4e0a\u81f3\u53f3\u4e0b)\uff1a<br \/>1. \u4f86\u81ea\u4e2d\u4ecb\u5b50 (intron) \u7684 miRNA \u5e8f\u5217\uff0c\u4e0d\u7d93\u904e Drosha\uff0c\u800c\u662f\u7531 RNA \u526a\u63a5\u53cd\u61c9\u5f62\u6210 pre-miRNA\u3002\u6b64\u5f8c\u7684\u6b65\u9a5f\u8207\u4e00\u822c\u8def\u5f91\u76f8\u540c\u3002<br \/>\u2014\u2013<br \/>siRNA \u8def\u5f91 (\u5716\u7247\u5de6\u5074\u81f3\u5de6\u4e0b)\uff1a<br \/>1. \u5916\u4f86\u96d9\u80a1 DNA \u9032\u5165\u7d30\u80de\u8cea\uff0c\u7531 Dicer \u5207\u5272\u70ba\u6578\u5341\u6838\u82f7\u9178\u9577\u5ea6\u7684\u96d9\u80a1 RNA \u3002<br \/>2. \u5176\u4e2d\u4e00\u80a1\u8207 AGO \u7b49\u86cb\u767d\u7d44\u6210 RISC\uff0c\u53ef\u4ee5\u548c\u76ee\u6a19 mRNA \u5b8c\u7f8e\u7d50\u5408\uff0c\u4e26\u5c07\u5176\u5207\u65b7\u964d\u89e3\u3002<br \/>\u8cc7\u6599\u4f86\u6e90\u2502\u8a79\u4e16\u9d6c\uff0c2010\u3002\u3008\u5c0f\u5175\u7acb\u5927\u529f\u2500 \u5fae\u5c0f\u6838\u91a3\u6838\u9178\u8207\u5c0f\u5e72\u64fe\u6838\u91a3\u6838\u9178\u3009\uff0c\u300a\u5316\u5b78\u300b\uff0c68 (4)\uff0c303 \u2013 312\u3002 \u5716\u8aaa\u91cd\u88fd\u2502\u6797\u4efb\u9060\u3001\u5f35\u8a9e\u8fb0<\/p><\/div>\n<p>&nbsp;<\/p>\n<p>\u5982\u4e0a\u5716\u6240\u793a\uff0cmiRNA \u7684\u751f\u6210\u5305\u542b\u5169\u7a2e\u6838\u91a3\u6838\u9178\u5167\u5207\u9176\uff1a Drosha \u8207 Dicer \uff0c\u63a5\u8457\u548c Argonaute \u86cb\u767d\u8cea\u7d44\u6210\u300c\u6838\u91a3\u6838\u9178\u6c89\u9ed8\u8907\u5408\u9ad4\u300d (RNA-induced silencing complex, RISC) \u6291\u5236 mRNA \u7684\u8f49\u8b6f\u4f5c\u7528\u6216\u4fc3\u4f7f\u5176\u964d\u89e3\u3002siRNA \u5247\u4f86\u81ea\u751f\u7269\u9ad4\u5916\u7684\u9577\u6bb5 DNA\uff0c\u7d93 Dicer \u5207\u5272\u800c\u4f86\uff0csiRNA \u4ea6\u53ef\u7d44\u6210 RISC \u5f15\u767c\u76ee\u6a19 mRNA \u964d\u89e3\u3002<\/p>\n<p>\u8a79\u4e16\u9d6c\u5728\u535a\u58eb\u5f8c\u7814\u7a76\u4e2d\uff0c\u5c31\u767c\u73fe\u7dda\u87f2\u7684 let-7 miRNA \u5f62\u6210\u7684\u6838\u91a3\u6838\u9178\u2500\u86cb\u767d\u8907\u5408\u9ad4\u5177\u6709\u5e8f\uf99c\u7279\uf962\u6027\uff0c\u67d0\u5e7e\u7a2e\u86cb\u767d\u8cea\u50c5\u8207 let-7 miRNA \u7684\u7279\u5b9a\u5e8f\uf99c\u7d50\u5408\uff0c\u986f\u793a miRNA \u5f62\u6210 RISC \u7684\u7279\u7570\u6027\u8207\u591a\u6a23\u6027\u3002<\/p>\n<p>\u6b64\u5916\uff0c\u7576 mRNA \u7684\u8f49\u8b6f\u4f5c\u7528\u88ab miRNA \u6291\u5236\u5f8c\uff0c\u4f86\u81ea RISC \u7684\u53bb\u817a\u5627\u5576\u9176(deadenylase) \u8907\u5408\u7269\u6703\u9032\u4e00\u6b65\u5f15\u767c mRNA \u964d\u89e3 (degradation)\u3002\u56e0\u6b64\u5728 miRNA \u4f5c\u7528\u6642\uff0c\u53ef\u4ee5\u89c0\u5bdf\u5230\u86cb\u767d\u8cea\u5408\u6210\u88ab\u6291\u5236\uff0c\u4e5f\u53ef\u4ee5\u767c\u73fe\u5230 mRNA \u6e1b\u5c11\u3002\u7136\u800c\uff0c\uf967\u540c miRNA \u5f15\u767c mRNA \u6e1b\u5c11\u7684\u5e45\ufa01\u8207\u901f\uf961\uff0c\u4e5f\u5728\u5404\u7a2e\u7814\u7a76\u4e2d\ufa26\u51fa\u73fe\u5dee\uf962\u3002<\/p>\n<p>\u8a79\u4e16\u9d6c\u8aaa\uff0c\u56e0\u70ba\u9019\u4e9b\u7279\u9ede\uff0cmiRNA \u5f88\u53ef\u80fd\u56e0\u70ba\u300c\u5e8f\uf99c\u5dee\uf962\u300d\uff0c\u4ee5\u53ca\u4e0d\u540c\u7684\u300c\u8f14\u52a9\u86cb\u767d\u5206\u5b50\u300d\u7d44\u5408\uff0c \u6c7a\u5b9a\u76ee\u6a19 mRNA \u7684\u547d\u904b\u3002\u4ed6\u5728\u7814\u7a76\u4e2d\u767c\u73fe\uff0c\u6838\ufa03\u9ad4\u4e0a\u7684\u7279\u5b9a\u86cb\u767d\u8cea\u6703\u5f71\u97ff let-7 miRNA \u529f\u80fd\uff0c\u8a72\u86cb\u767d\u8cea\u6240\u5728\u7684\u6838\ufa03\u9ad4\u5340\u57df\u5f88\u53ef\u80fd\u662f miRNA \u767c\u63ee\u6291\u5236\u4f5c\u7528\u4e4b\u8655\u3002<\/p>\n<p>\u81f3\u4eca\uff0c\u8a79\u4e16\u9d6c\u4ecd\u7136\u5e38\u5e38\u505a\u5be6\u9a57\u5230\u4e09\u66f4\u534a\u591c\uff0c\u81f4\u529b\u5c07\u9019\u4e9b\u8907\u96dc\u4e14\u7cbe\u5fae\u7684\u53cd\u61c9\uff0c\u7814\u7a76\u5730\u66f4\u52a0\u900f\u5fb9\u3002\u70ba\u7684\u662f\u597d\u5947\u5fc3\uff0c\u4e5f\u70ba\u4e86\u6709\u671d\u4e00\u65e5\u80fd\u7acb\u57fa\u65bc\u9019\u4e9b\u77e5\u8b58\u767c\u5c55\u91ab\u85e5\u61c9\u7528\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2><strong>\u66f4\u591a\u7cbe\u91c7\u6587\u7ae0\uff0c\u8acb\u81f3\u300c\u7814\u4e4b\u6709\u7269\u300d\u5b98\u7db2\uff1a<a href=\"http:\/research.sinica.edu.tw\/\"><em>http:\/research.sinica.edu.tw\/<\/em><\/a><\/strong><\/h2>\n","protected":false},"excerpt":{"rendered":"<p>miRNA \u7814\u7a76\u70ba\u4f55\u91cd\u8981 miRNA \u53ef\u8abf\u63a7\u8a31\u591a\u751f\u7269\u7684\u57fa\u56e0\u8868\u73fe\uff0c\u4e26\u5f71\u97ff\u4eba\u985e\u764c\u75c7\u7684\u767c\u751f\u6a5f\u5236\uff0c\u662f\u73fe\u4ee3\u91ab\u7642\u7684\u95dc\u9375\u5206\u5b50 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":14369,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[4],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/14368"}],"collection":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/comments?post=14368"}],"version-history":[{"count":1,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/14368\/revisions"}],"predecessor-version":[{"id":16282,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/14368\/revisions\/16282"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media\/14369"}],"wp:attachment":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media?parent=14368"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/categories?post=14368"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/tags?post=14368"}],"curies":[{"name":"wp","href":"https:\/api.w.org\/{rel}","templated":true}]}}