{"id":9726,"date":"2022-06-02T00:17:18","date_gmt":"2022-06-01T16:17:18","guid":{"rendered":"https:\/\/newsletter.sinica.edu.tw/en\/?p=9726"},"modified":"2024-03-13T09:19:05","modified_gmt":"2024-03-13T01:19:05","slug":"breakthrough-of-wild-tomato-protoplast-regeneration-and-dna-free-crispr-cas9-genome-editing","status":"publish","type":"post","link":"https:\/\/newsletter.sinica.edu.tw/en\/9726\/","title":{"rendered":"Breakthrough of wild tomato protoplast regeneration and DNA-free CRISPR-Cas9 genome editing."},"content":{"rendered":"<p>Wild tomato (Solanum peruvianum) is an important genetic source for the tomato breeding, but the progress has been hampered due to the self-incompatibility and highly heterozygous genome. Yao-Cheng Lin, Choun-Sea Lin, and Ming-Che Shih from Agricultural Biotechnology Research Center developed a DNA-free CRISPR-Cas9 genome editing tool based on an optimized protoplast regeneration protocol that creates stable and inheritable diploid and tetraploid regenerants. The study was published in <em>Plant Physiology<\/em> and selected as the cover image for the April Issue.<\/p>\n<p>Article link\uff1a<a href=\"https:\/\/doi.org\/10.1093\/plphys\/kiac022\">https:\/\/doi.org\/10.1093\/plphys\/kiac022<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-9728 aligncenter\" src=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-771x1024.jpg\" alt=\"\" width=\"771\" height=\"1024\" srcset=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-771x1024.jpg 771w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-226x300.jpg 226w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-768x1019.jpg 768w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-1157x1536.jpg 1157w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-1543x2048.jpg 1543w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-1080x1434.jpg 1080w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/\u76f4\u5f0f\u8fb2\u751f\u4e2d\u5fc3\u6797\u8000\u6b63-scaled.jpg 1929w\" sizes=\"(max-width: 771px) 100vw, 771px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-9727 aligncenter\" src=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-1024x615.jpg\" alt=\"\" width=\"1024\" height=\"615\" srcset=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-1024x615.jpg 1024w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-300x180.jpg 300w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-768x462.jpg 768w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-1080x649.jpg 1080w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-1280x768.jpg 1280w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-627x376.jpg 627w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11-440x264.jpg 440w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2022\/05\/webimage-11.jpg 1396w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Wild tomato (Solanum peruvianum) is an important genetic source for the tomato breeding, but the progress has been hampered due to the self-incompatibility and highly heterozygous genome. Yao-Cheng Lin, Choun-Sea Lin, and Ming-Che Shih from Agricultural Biotechnology Research Center developed a DNA-free CRISPR-Cas9 genome editing tool based on an optimized protoplast regeneration protocol that creates [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":9727,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[533],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/9726"}],"collection":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/comments?post=9726"}],"version-history":[{"count":2,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/9726\/revisions"}],"predecessor-version":[{"id":13194,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/9726\/revisions\/13194"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media\/9727"}],"wp:attachment":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media?parent=9726"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/categories?post=9726"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/tags?post=9726"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}