{"id":6453,"date":"2019-11-14T00:00:40","date_gmt":"2019-11-13T16:00:40","guid":{"rendered":"https:\/\/newsletter.sinica.edu.tw/en\/?p=6453"},"modified":"2021-08-26T13:34:26","modified_gmt":"2021-08-26T05:34:26","slug":"three-dimensional-optical-super-resolution-microscopy-microscopes-image-a-whole-fly-brain","status":"publish","type":"post","link":"https:\/\/newsletter.sinica.edu.tw/en\/6453\/","title":{"rendered":"Three-Dimensional Optical Super-Resolution Microscopy: Microscopes Image a Whole Fly Brain"},"content":{"rendered":"
The 2014 Nobel Prize in Chemistry was awarded to three scientists who have advanced the optical microscopy system beyond the limits of optical resolution. Assistant Research Fellow Bi-Chang Chen, from the Academia Sinica Applied Science Research Center, and Academician Ann-Shyn Chiang, Dean of the Department of Life Sciences and Brain Research Center of Tsing Hua University, jointly developed a new generation of three-dimensional optical super-resolution microscopy – Microscopes allow super resolution imaging from the cell level to the tissue level, which deconstructing the dopaminergic neural network of the whole brain of Drosophila<\/em>, and seeing the regeneration of memory proteins at specific neuronal synapses. The results of this research have been published in Nature Communications<\/em> on October 18, 2019.<\/p>\n Before coming back to the Academia Sinica, Bi-Chang Chen worked in Dr. Eric Betzig\u2019s lab, one of the winners of the 2014 Nobel Prize in Chemistry. Recently, he cooperates with Academician Ann-Shyn Chiang to make biological tissues transparent under the microscope, which enables locating and quantifying the spatial distribution of each single protein. This technology is expected to bring breakthroughs in tissue physiology and pathology research and has the potential to uncover the mystery of the brain’s memory mechanism.<\/p>\n Bi-Chang Chen said that this research results can be regarded as an advanced version of his research in Communications Biology earlier this year. The study used a lightsheet localization microscope to achieve a three-dimensional spatial resolution of less than 100 nm, and the size of the nuclear pores was visible.<\/p>\n