{"id":12584,"date":"2023-09-21T00:03:17","date_gmt":"2023-09-20T16:03:17","guid":{"rendered":"https:\/\/newsletter.sinica.edu.tw/en\/?p=12584"},"modified":"2024-03-13T09:19:02","modified_gmt":"2024-03-13T01:19:02","slug":"structural-convergence-endows-nuclear-transport-receptor-kap114p-with-a-transcriptional-repressor-function-toward-tata-binding-protein","status":"publish","type":"post","link":"https:\/\/newsletter.sinica.edu.tw/en\/12584\/","title":{"rendered":"Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein"},"content":{"rendered":"<p>The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-\u03b2 (Kap-\u03b2) in yeast, and various regulatory factors. Previous studies showed that Kap114p, a Kap-\u03b2 that mediates nuclear import of yeast TBP (yTBP), modulates yTBP-dependent transcription. However, how Kap114p associates with yTBP to exert its multifaceted functions has remained elusive. The team led by Dr. Kuo-Chiang Hsia (Institute of Molecular Biology, Academia Sinica) and Dr. Wei-Yi Chen (Institute of Biochemistry and Molecular Biology, College of Life Sciences, National Yang Ming Chiao Tung University) presents a cryo-EM structure of Kap114p, one of the Kap-\u03b2s, in complex with TATA box binding protein and reveals a non-canonical function beyond nuclear transport that modulates TBP-dependent transcription. This study has been published in in the journal <em>Nature Communications<\/em>.<\/p>\n<p>Article link: <a href=\"https:\/\/www.nature.com\/articles\/s41467-023-41206-9\" rel=\"noopener\" target=\"_blank\">https:\/\/www.nature.com\/articles\/s41467-023-41206-9<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-1.jpg\" alt=\"Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein \" width=\"900\" height=\"600\" class=\"aligncenter size-full wp-image-12585\" srcset=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-1.jpg 900w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-1-300x200.jpg 300w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-1-768x512.jpg 768w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-\u03b2 (Kap-\u03b2) in yeast, and various regulatory factors. Previous studies showed that Kap114p, a Kap-\u03b2 that mediates nuclear import of yeast TBP (yTBP), modulates yTBP-dependent transcription. However, how Kap114p associates with yTBP [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":12586,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[533],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12584"}],"collection":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/comments?post=12584"}],"version-history":[{"count":1,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12584\/revisions"}],"predecessor-version":[{"id":13136,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12584\/revisions\/13136"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media\/12586"}],"wp:attachment":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media?parent=12584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/categories?post=12584"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/tags?post=12584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}