{"id":33473,"date":"2023-12-28T00:13:12","date_gmt":"2023-12-27T16:13:12","guid":{"rendered":"https:\/newsletter.sinica.edu.tw\/?p=33473"},"modified":"2023-12-28T00:07:03","modified_gmt":"2023-12-27T16:07:03","slug":"%e3%80%90%e5%b0%88%e6%ac%84%e3%80%91%e5%9f%ba%e5%9b%a0%e7%b7%a8%e8%bc%af%e8%88%87%e5%8e%9f%e7%94%9f%e8%b3%aa%e9%ab%94%e5%86%8d%e7%94%9f%ef%bc%9a%e8%be%b2%e6%a5%ad%e7%9a%84%e5%b6%84%e6%96%b0%e6%87%89","status":"publish","type":"post","link":"https:\/newsletter.sinica.edu.tw\/33473\/","title":{"rendered":"\u3010\u5c08\u6b04\u3011\u57fa\u56e0\u7de8\u8f2f\u8207\u539f\u751f\u8cea\u9ad4\u518d\u751f\uff1a\u8fb2\u696d\u7684\u5d84\u65b0\u61c9\u7528"},"content":{"rendered":"<div class=\"auth-info\">\n<div class='one_fourth'>\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_A.jpg\" alt=\"\u6797\u5d07\u7199\u535a\u58eb\" width=\"150\" height=\"150\" class=\"aligncenter size-full wp-image-33474\" \/>\n\t\t\t\t<\/div>\n<div class='three_fourth et_column_last'>\n\t\t\t\t\t\u6797\u5d07\u7199\u535a\u58eb\u76ee\u524d\u70ba\u672c\u9662\u8fb2\u696d\u751f\u7269\u79d1\u6280\u7814\u7a76\u4e2d\u5fc3\u690d\u7269\u6838\u5fc3\u5be6\u9a57\u5ba4\u4e3b\u6301\u4eba\uff0c\u5c08\u9577\u65bc\u5efa\u7acb\u690d\u7269\u7814\u7a76\u76f8\u95dc\u5de5\u5177\u5e73\u53f0\u53ca\u4e0d\u540c\u7269\u7a2e\u7d44\u7e54\u57f9\u990a\u8207\u539f\u751f\u8cea\u9ad4\u7684\u6280\u8853\u61c9\u7528\u3002\u8a72\u6838\u5fc3\u5be6\u9a57\u5ba4\u5728\u591a\u7269\u7a2e\u539f\u751f\u8cea\u9ad4\u57fa\u56e0\u7de8\u8f2f\u8207\u518d\u751f\u6280\u8853\u7814\u7a76\uff0c\u56e0\u70ba\u4e0d\u5177\u6709\u57fa\u56e0\u8f49\u6b96\u7684\u5916\u4f86\u5e8f\u5217\u4e14\u6240\u7522\u751f\u7684\u690d\u7269\u53ef\u9054\u5230\u76ee\u6a19\u7de8\u8f2f\uff0c\u53ef\u80fd\u53ef\u4ee5\u89e3\u6c7a\u5927\u773e\u5c0d\u65bc\u57fa\u56e0\u8f49\u6b96\u4e0a\u7684\u7591\u616e\u3002\n\t\t\t\t<\/div><div class='clear'><\/div>\n<\/div>\n<p>&nbsp;<\/p>\n<p>\u4eba\u985e\u81ea\u4e00\u842c\u5169\u5343\u5e74\u524d\u958b\u59cb\u99b4\u5316\u3001\u9078\u64c7\u9069\u5408\u683d\u57f9\u7684\u7ce7\u98df\u4f5c\u7269\uff0c\u4e26\u4e14\u900f\u904e\u6230\u722d\u3001\u90e8\u843d\u9077\u5f99\u4ee5\u53ca\u5546\u696d\u884c\u70ba\uff0c\u5c0b\u627e\u66f4\u5408\u9069\u7684\u683d\u57f9\u74b0\u5883\u8207\u6539\u826f\u66f4\u7b26\u5408\u98df\u7528\u50f9\u503c\u7684\u54c1\u7a2e\u3002\u81f3\u4eca\uff0c\u70ba\u4e86\u56e0\u61c9\u74b0\u5883\u8b8a\u9077\u8207\u98df\u7528\u559c\u597d\uff0c\u4eba\u985e\u4ecd\u7136\u6301\u7e8c\u8457\u99b4\u5316\u3001\u5f15\u7a2e\u3001\u9078\u80b2\u66f4\u9069\u5408\u683d\u57f9\u8207\u5546\u696d\u9700\u6c42\u7684\u65b0\u54c1\u7a2e\u4f86\u89e3\u6c7a\u4eba\u985e\u5403\u7684\u554f\u984c\u3002<\/p>\n<p>\u806f\u5408\u570b\u6c23\u5019\u8b8a\u9077\u5c08\u9580\u59d4\u54e1\u6703\uff08IPCC\uff09\u7b2c\u516d\u6b21\u8a55\u4f30\u5831\u544a\u4e2d\u63d0\u51fa\uff0c\u96a8\u8457\u5168\u7403\u6696\u5316\uff0c\u9810\u4f30\u7d04\u6709 33-36 \u5104\u4eba\u53e3\u751f\u6d3b\u5728\u6975\u6613\u53d7\u6c23\u5019\u8b8a\u5316\u5f71\u97ff\u7684\u74b0\u5883\u4e2d\uff0c\u751f\u614b\u5e73\u8861\u8207\u7ce7\u98df\u4f9b\u9700\u90fd\u5c07\u53d7\u5230\u5a01\u8105 <sup>1<\/sup>\u3002\u904e\u53bb\u70ba\u4e86\u6311\u9078\u9069\u61c9\u74b0\u5883\u6216\u7b26\u5408\u5e02\u5834\u53d6\u5411\u7684\u54c1\u7a2e\uff0c\u5229\u7528\u4eba\u5de5\u6388\u7c89\u7b49\u80b2\u7a2e\u7684\u6280\u8853\u5c07\u512a\u826f\u7279\u5fb5\u9010\u6b65\u6539\u5584\uff0c\u7d93\u904e\u6578\u4ee3\u7684\u4eba\u70ba\u9078\u64c7\uff0c\u53ef\u4ee5\u7372\u5f97\u5e36\u6709\u76ee\u6a19\u7279\u5fb5\u7684\u65b0\u54c1\u7a2e\u3002\u9019\u6a23\u7684\u9078\u80b2\u6d41\u7a0b\u901a\u5e38\u9700\u8981\u8017\u8cbb\u4f5c\u7269 6-7 \u500b\u4e16\u4ee3\u624d\u80fd\u8b93\u4e00\u500b\u76ee\u6a19\u7279\u5fb5\u7a69\u5b9a\u907a\u50b3\uff0c\u53ef\u80fd\u9700\u8981\u82b1\u8cbb\u6578\u5e74\u751a\u81f3\u6578\u5341\u5e74\u624d\u5c07\u591a\u500b\u7279\u5fb5\u540c\u6642\u52a0\u5230\u5230\u65b0\u54c1\u7a2e\u4e0a\uff0c\u9019\u4e5f\u610f\u5473\u8457\u5982\u4f55\u958b\u767c\u65b0\u7684\u6280\u8853\u4f86\u52a0\u901f\u80b2\u7a2e\u7684\u5de5\u4f5c\uff0c\u4ee5\u5feb\u901f\u56e0\u61c9\u74b0\u5883\u8b8a\u9077\u8207\u5e02\u5834\u559c\u597d\u7684\u8b8a\u5316\u662f\u4e00\u500b\u91cd\u8981\u7684\u8ab2\u984c\u3002<\/p>\n<p><strong>\u65b0\u578b\u614b\u57fa\u56e0\u7de8\u8f2f\u6280\u8853CRISPRC\/Cas \u52a0\u901f\u690d\u7269\u5206\u5b50\u80b2\u7a2e\u8173\u6b65<\/strong><\/p>\n<p>\u904e\u53bb\u5341\u5e74\uff0c\u7814\u7a76\u4eba\u54e1\u5229\u7528\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u4f86\u300c\u589e\u52a0\u3001\u522a\u9664\u6216\u6539\u8b8a\u300d\u751f\u7269\u7684 DNA \u5e8f\u5217\uff0c\u4f7f\u5f97\u57fa\u56e0\u7de8\u8f2f\u5728 1-2 \u500b\u4e16\u4ee3\u5c31\u80fd\u9054\u5230\u985e\u4f3c\u81ea\u7136\u754c\u6f14\u5316\u3001\u57fa\u56e0\u7a81\u8b8a\u6216\u662f\u96dc\u4ea4\u7684\u76ee\u7684\uff0c\u9019\u4e9b\u6280\u8853\u8b93\u751f\u7269\u5b78\u767c\u751f\u4e86\u5de8\u5927\u7684\u8b8a\u5316\u3002CRISPR\/Cas\uff08Clustered Regularly Interspaced Short Palindromic Repeats\/CRISPR-associated proteins\uff0c\u5e38\u9593\u56de\u6587\u91cd\u8907\u5e8f\u5217\u53e2\u96c6\uff0f\u5e38\u9593\u56de\u6587\u91cd\u8907\u5e8f\u5217\u53e2\u96c6\u95dc\u806f\u86cb\u767d\u7cfb\u7d71\uff09\u57fa\u56e0\u5de5\u7a0b\u7cfb\u7d71\u7684\u5efa\u7acb\uff0c\u555f\u767c\u4e86\u751f\u7269\u5b78\u5bb6\u7121\u9650\u7684\u5275\u9020\u529b\u3002\u53d7\u81ea\u7136\u754c\u5b58\u5728\u7684 CRISPR\/Cas \u86cb\u767d\u555f\u767c\uff0c\u5176\u7c21\u4fbf\u7684\u8a2d\u8a08\u548c\u6613\u65bc\u50b3\u9001\u5230\u7d30\u80de\u4e2d\u7684\u7279\u9ede\uff0c\u4fc3\u4f7f\u79d1\u5b78\u5bb6\u4e0d\u65b7\u958b\u767c\u5177\u6709\u4e0d\u540c\u529f\u80fd\u7684 CRISPR\/Cas \u86cb\u767d\u8cea\u4f86\u7cbe\u78ba\u5207\u5272\u548c\u7c98\u8cbc DNA\u3002<\/p>\n<p>CRISPR\/Cas \u86cb\u767d\u662f\u4e00\u7a2e DNA \u526a\u5207\u9176\uff0c\u53ef\u4ee5\u548c\u5f15\u5c0e RNA \u7d50\u5408\u6210\u8907\u5408\u9ad4\uff0c\u4e26\u641c\u7d22\u67d3\u8272\u9ad4\u4e0a\u8207 RNA \u5e8f\u5217\u4e92\u88dc\u7684\u76ee\u6a19 DNA\uff08\u627e\u5230\u60f3\u6539\u8b8a\u7684\u57fa\u56e0\u4f4d\u7f6e\uff09\uff0c\u7cbe\u6e96\u526a\u5207\u7de8\u8f2f DNA \u5e8f\u5217\uff08\u4e0d\u6703\u5f71\u97ff\u5230\u5176\u4ed6\u4e0d\u60f3\u6539\u8b8a\u7684\u5730\u65b9\uff09\u3002\u5728\u81ea\u7136\u754c\uff0c\u7d30\u83cc\u70ba\u4e86\u62b5\u79a6\u55dc\u83cc\u9ad4\u9019\u4e00\u985e\u7684\u75c5\u6bd2\u800c\u767c\u5c55\u51fa\u4f86\u7684\u9632\u79a6\u6a5f\u5236\u3002\u75c5\u6bd2\u5165\u4fb5\u7d30\u83cc\u5f8c\uff0c\u5229\u7528\u7d30\u83cc\u9ad4\u5167\u7684\u539f\u6599\u6301\u7e8c\u81ea\u6211\u8907\u88fd\uff0c\u6700\u7d42\u5c0e\u81f4\u7d30\u83cc\u6b7b\u4ea1\u3002\u505a\u70ba\u56de\u61c9\uff0c\u4e00\u4e9b\u7d30\u83cc\u6f14\u5316\u51fa\u53cd\u64ca\u7684\u624b\u6bb5\uff0c\u5b83\u5011\u6f14\u5316\u51fa DNA \u526a\u5207\u9176\uff0c\u4e00\u65e6\u767c\u73fe\u6709\u75c5\u6bd2\u57fa\u56e0\u5165\u4fb5\uff0c\u5c31\u5c07\u5176 DNA \u5207\u788e\uff0c\u7576\u9019\u4e9b\u7d30\u83cc\u5728\u75c5\u6bd2\u653b\u64ca\u4e2d\u5016\u5b58\u4e0b\u4f86\u5f8c\uff0c\u5b83\u5011\u6703\u628a\u75c5\u6bd2 DNA \u7684\u5c0f\u7247\u6bb5\u5d4c\u5165\u81ea\u5df1\u7684\u57fa\u56e0\u7d44\u5efa\u6a94\u6210\u8cc7\u6599\u5eab\uff0c\u9019\u4e9b\u75c5\u6bd2\u4ee3\u78bc\u7247\u6bb5\uff08\u5c07\u5f62\u6210\u5f15\u5c0e RNA\uff09\u4f7f\u7d30\u83cc\u8a18\u61b6\u904e\u53bb\u653b\u64ca\u904e\u81ea\u5df1\u7684\u6575\u4eba\u3002\u7576\u75c5\u6bd2\u4e0b\u6b21\u518d\u653b\u64ca\u6642\uff0c\u7d30\u83cc\u53ef\u4ee5\u7528\u4e00\u7a2e\u66f4\u5f3a\u5927\u7684\u526a\u5207\u9176-CRISPR\/Cas \u86cb\u767d\u6709\u6548\u7387\u7684\u74e6\u89e3\u75c5\u6bd2\u653b\u64ca\u3002<\/p>\n<p>\u7814\u7a76\u4eba\u54e1\u5f9e\u81ea\u7136\u754c\u7684\u555f\u767c\u4fee\u6539\u4e86 CRISPR\/Cas \u6838\u9178\u526a\u5207\u7cfb\u7d71\uff0c\u7814\u7a76\u4eba\u54e1\u61c9\u7528\u9019\u4e9b\u86cb\u767d\u8cea\u4f7f\u7528\u5728\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u3002Jennifer Anne Doudna \u8207 Emmanuelle Marie Charpentier \u66f4\u56e0\u6b64\u6280\u8853\u7684\u958b\u767c\u7372\u5f97 2020 \u5e74\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u3002\u73fe\u4eca\u6700\u88ab\u5ee3\u6cdb\u61c9\u7528\u5728\u690d\u7269\u79d1\u5b78\u7814\u7a76\u7684\u662f CRISPR\/Cas9 \u4e8c\u805a\u9ad4 DNA \u526a\u5207\u9176\u3002<\/p>\n<p>\u64cd\u4f5c CRISPR\/Cas \u57fa\u56e0\u7de8\u8f2f\u7cfb\u7d71\u7b2c\u4e00\u6b65\u662f\u5c0b\u627e\u76ee\u6a19\u57fa\u56e0\u4e0a CRISPR\/Cas9 \u86cb\u767d\u8fa8\u8b58\u4f4d PAM\uff08protospacer adjacent motif\uff09\u9130\u8fd1\u5340\u57df\u7684 DNA \u5e8f\u5217\uff0c\u4e26\u8a2d\u8a08\u5408\u6210\u8207 DNA \u5e8f\u5217\u4e92\u88dc\u7684\u55ae\u93c8\u5f15\u5c0e RNA\uff08sgRNA\uff09\uff0c\u76ee\u6a19\u53ef\u4ee5\u662f\u7d30\u80de\u6838\u67d3\u8272\u9ad4\u4e0a\u7684\u4efb\u4e00\u5340\u57df\uff0c \u56e0\u6b64\u7121\u8ad6\u662f\u751f\u7269\u7684\u907a\u50b3\u7f3a\u9677\u9084\u662f\u4e0d\u53d7\u6b61\u8fce\u7684\u7279\u5fb5\u90fd\u53ef\u4ee5\u9032\u884c\u526a\u5207\u7de8\u8f2f\u3002\u7136\u5f8c\u5c07\u9019\u7a2e\u6975\u77ed\u5e8f\u5217\u7684 sgRNA \u8207 Cas9 \u86cb\u767d\u7d44\u5408\u6210 RNP \u8907\u5408\u9ad4\uff08Ribonucleoprotein\uff09\u5f8c\uff0c\u9032\u5165\u76ee\u6a19\u7de8\u8f2f\u7d30\u80de\u4e2d\u3002RNP \u8907\u5408\u9ad4\u4e0a\u7684 CRISPR\/Cas \u641c\u7d22 PAM \u5340\u57df\uff0c\u4f9d\u64da sgRNA \u7684\u5e8f\u5217\u6383\u63cf\u67d3\u8272\u9ad4\u4e0a\u53ef\u4ee5\u914d\u5c0d\u7684 DNA \u5e8f\u5217\u5f8c\uff0cCRISPR\/Cas \u4e8c\u805a\u9ad4\u86cb\u767d\u5c31\u5982\u540c\u526a\u5200\u7684\u5169\u7247\u5200\u5203\uff0c\u5c07\u76ee\u6a19 DNA \u4f4d\u7f6e\u526a\u65b7\u3002<\/p>\n<p>\u88ab\u526a\u65b7\u7684 DNA \u5728\u81ea\u6211\u4fee\u5fa9\u904e\u7a0b\u4e2d\u53ef\u80fd\u56e0\u70ba\u907a\u5931\u5c0f\u7247\u6bb5\u7684 DNA\uff0c\u4f7f\u5f97\u907a\u50b3\u7f3a\u9677\u6216\u662f\u4e0d\u53d7\u6b61\u8fce\u7684\u7279\u5fb5\u7121\u6cd5\u5728\u4e0b\u4e00\u4ee3\u8868\u73fe\uff1b\u6216\u662f\u5728 DNA \u81ea\u6211\u4fee\u88dc\u7684\u904e\u7a0b\u4e2d\u5c0b\u627e\u9130\u8fd1\u7684 DNA \u505a\u70ba\u6a21\u677f\uff0c\u5728\u526a\u65b7\u7684\u5207\u53e3\u4e2d\u589e\u52a0\u4e00\u6bb5\u65b0\u7684 DNA \u5e8f\u5217\u4e26\u9032\u800c\u7372\u5f97\u65b0\u7684\u907a\u50b3\u7279\u5fb5 <sup>2<\/sup>\u3002<\/p>\n<p><strong>CRISPR\/Cas \u89e3\u6c7a GMO \u6cd5\u898f\u9650\u5236\u8207\u98df\u7528\u5b89\u5168\u7591\u616e<\/strong><\/p>\n<p>\u96d6\u7136\u7f8e\u570b\u8fb2\u696d\u90e8\u5df2\u7d93\u51c6\u8a31\u5c11\u6578\u57fa\u56e0\u6539\u9020\u8fb2\u7522\u54c1\u4e0a\u5e02\uff0c\u4f46\u6b50\u76df\u6216\u4e9e\u6d32\u570b\u5bb6\u7684\u653f\u7b56\u8207\u6d88\u8cbb\u65cf\u7fa4\u5c0d\u65bc\u67d3\u8272\u9ad4\u4e2d\u542b\u6709\u5916\u6e90\u7269\u7a2e DNA \u7247\u6bb5\u7684\u57fa\u56e0\u6539\u9020\u4f5c\u7269\u4ecd\u7136\u62b1\u6301\u8457\u8b39\u614e\u4fdd\u5b88\u7684\u614b\u5ea6\u3002\u904e\u53bb\u4f9d\u8cf4\u8fb2\u687f\u83cc\u6216\u57fa\u56e0\u69cd\u5c0e\u5165\u5916\u6e90\u7269\u7a2e DNA \u7684\u57fa\u56e0\u6539\u9020\u4f5c\u7269\u96d6\u7136\u53ef\u4ee5\u5927\u5e45\u7e2e\u77ed\u76ee\u6a19\u907a\u50b3\u7279\u5fb5\u7d2f\u7a4d\u6240\u8cbb\u6642\u9593\uff0c\u4f46\u5176\u683d\u57f9\u74b0\u5883\u53d7\u5230\u975e\u5e38\u56b4\u8b39\u7684\u6cd5\u898f\u76e3\u7ba1\uff0c\u66f4\u9051\u8ad6\u6210\u70ba\u5546\u696d\u54c1\u7a2e\u4e0a\u5e02\u3002CRISPR\/Cas \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u7684\u6210\u719f\uff0c\u4e14\u53ea\u91dd\u5c0d\u7279\u5b9a\u7684\u5340\u57df\u9032\u884c\u7a81\u8b8a\uff0c\u9019\u7a2e\u7a81\u8b8a\u662f\u5728\u81ea\u7136\u754c\u4e2d\u53ef\u80fd\u53ef\u4ee5\u767c\u751f\u7684\u3002<\/p>\n<p>\u56e0\u6b64\uff0c\u57fa\u56e0\u7de8\u8f2f\u4f5c\u7269\u8207\u542b\u6709\u5916\u4f86\u57fa\u56e0\u7684\u57fa\u56e0\u8f49\u6b96\u4e0d\u540c\uff0c\u53ef\u80fd\u53ef\u4ee5\u89e3\u6c7a\u57fa\u56e0\u6539\u9020\u4f5c\u7269\u6240\u9762\u81e8\u7684\u6cd5\u898f\u76e3\u7ba1\u56f0\u5883\u4ee5\u53ca\u6d88\u8cbb\u8005\u7684\u98df\u5b89\u7591\u616e\u3002\u4f46\u662f\u5927\u591a\u6578\u7684\u4f5c\u7269\u57fa\u56e0\u7de8\u8f2f\u65b9\u6cd5\u90fd\u662f\u4f7f\u7528\u57fa\u56e0\u8f49\u6b96\u7684\u65b9\u6cd5\uff0c\u9700\u8981\u628a\u76f8\u95dc\u6240\u9700\u8981\u7684\u7de8\u8f2f\u5de5\u5177\u9001\u5230\u67d3\u8272\u9ad4\u4e2d\uff08\u5f62\u6210\u57fa\u56e0\u8f49\u6b96\u690d\u7269\uff09\uff0c\u7b49\u57fa\u56e0\u7de8\u8f2f\u5b8c\u6210\u5f8c\uff0c\u56e0\u70ba\u5df2\u4e0d\u518d\u9700\u8981\u57fa\u56e0\u7de8\u8f2f\u7684\u5de5\u5177\uff0c\u518d\u4ee5\u4ea4\u914d\u7684\u65b9\u5f0f\u628a\u9019\u4e9b\u5de5\u5177\u53bb\u9664\u3002\u4f46\u9019\u500b\u65b9\u5f0f\u56e0\u70ba\u4e00\u958b\u59cb\u4ecd\u9700\u8981\u57fa\u56e0\u8f49\u6b96\uff0c\u6240\u4ee5\u9020\u6210\u7121\u6cd5\u61c9\u7528\u5230\u4e00\u4e9b\u5f88\u4e45\u624d\u80fd\u958b\u82b1\u7684\u4f5c\u7269\uff08\u5982\u679c\u6a39\uff09\uff0c\u6216\u662f\u7121\u6cd5\u7d50\u7a2e\uff08\u5982\u9999\u8549\uff09\u7684\u4f5c\u7269\u4e0a\u3002\u70ba\u4e86\u89e3\u6c7a\u9019\u6a23\u7684\u554f\u984c\uff0c\u6211\u5011\u5be6\u9a57\u5ba4\u8207\u4e16\u754c\u4e0a\u5176\u4ed6\u7684\u5be6\u9a57\u5ba4\u4f7f\u7528\u539f\u751f\u8cea\u9ad4\u518d\u751f\u4f5c\u70ba\u7b56\u7565\u3002\u7d50\u5408 CRISPR\/Cas \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u53ca\u690d\u7269\u7d30\u80de\u53ef\u5168\u80fd\u5206\u5316\uff08totipotency\uff09\u7684\u7279\u6027\uff0c\u5efa\u7acb\u4e86\u591a\u7a2e\u690d\u7269\u4e0d\u5177\u6709\u5916\u6e90\u57fa\u56e0\u9022\u6a5f\u63d2\u5165\uff08\u4e0d\u9700\u8981\u5f62\u6210\u57fa\u56e0\u8f49\u6b96\u690d\u7269\uff09\u3001\u4e26\u4e14\u53ef\u540c\u6642\u591a\u57fa\u56e0\u3001\u591a\u76ee\u6a19\u5e8f\u5217\u767c\u751f\u7de8\u8f2f\u7684\u7d30\u80de\u518d\u751f\u7cfb\u7d71\u3002\u76f8\u95dc\u7684\u539f\u7406\u5982\u4e0b\uff1a<\/p>\n<p>\u8207\u52d5\u7269\u7d30\u80de\u4e0d\u540c\uff0c\u690d\u7269\u7d30\u80de\u6709\u7d30\u80de\u58c1\uff0c\u6240\u4ee5\u8981\u9032\u884c\u57fa\u56e0\u7de8\u8f2f\u6642\u7121\u6cd5\u76f4\u63a5\u628a\u5de5\u5177\u9001\u5230\u7d30\u80de\u5167\u3002\u70ba\u4e86\u4e0d\u963b\u64cb CRISPR\/Cas \u86cb\u767d\u8207\u5f15\u5c0e RNA \u6240\u5f62\u6210\u7684 RNP \u57fa\u56e0\u7de8\u8f2f\u8907\u5408\u9ad4\uff08\u57fa\u56e0\u7de8\u8f2f\u5de5\u5177\uff09\u904b\u8f38\u5230\u690d\u7269\u7d30\u80de\u4e2d\uff0c\u6211\u5011\u5c07\u690d\u7269\u5730\u4e0a\u90e8\u5668\u5b98\uff08\u5982\u8449\u7247\u3001\u8396\u3001\u80da\u8ef8\uff09\u7684\u7d30\u80de\u58c1\u7528\u7e96\u7dad\u9176\u8207\u679c\u81a0\u9176\u5206\u89e3\u4e26\u7372\u5f97\u539f\u751f\u8cea\u9ad4\u7d30\u80de\uff0c\u8b93 RNP \u8907\u5408\u9ad4\u53ef\u4ee5\u9806\u5229\u7a7f\u904e\u7d30\u80de\u819c\u5b54\u9699\uff0c\u91dd\u5c0d\u539f\u751f\u8cea\u9ad4\u7d30\u80de\u4e2d\u7684\u76ee\u6a19\u57fa\u56e0\u5e8f\u5217\u767c\u751f\u526a\u5207\u8207\u7de8\u8f2f\uff0c\u63a5\u8457\u57fa\u56e0\u7de8\u8f2f RNP \u8907\u5408\u9ad4\u529f\u6210\u8eab\u9000\uff0c\u96a8\u5373\u5728\u7d30\u80de\u8cea\u4e2d\u4ee3\u8b1d\u5206\u89e3\u3002\u56e0\u70ba\u57fa\u56e0\u7de8\u8f2f\u5de5\u5177\u4e0d\u5177\u6709 DNA\uff08\u53ea\u6709\u86cb\u767d\u8cea\u53ca RNA\uff09\uff0c\u56e0\u6b64\u4e0d\u5b58\u5728\u9022\u6a5f\u63d2\u5165\u690d\u7269\u7d30\u80de\u67d3\u8272\u9ad4\u3001\u7522\u751f\u57fa\u56e0\u6539\u9020\u7684\u98a8\u96aa\u3002<\/p>\n<p>\u9019\u4e9b\u7d93\u904e\u57fa\u56e0\u7de8\u8f2f\u7684\u539f\u751f\u8cea\u9ad4\u56e0\u812b\u96e2\u690d\u7269\u7279\u5b9a\u5668\u5b98\u6216\u7d44\u7e54\u5340\u57df\u7684\u751f\u7406\u74b0\u5883\u8207\u7d30\u80de\u58c1\u7684\u675f\u7e1b\u5f8c\uff0c\u6211\u5011\u4f9d\u64da\u4e0d\u540c\u7269\u7a2e\u7684\u539f\u751f\u8cea\u9ad4\u767c\u80b2\u7279\u6027\uff0c\u8abf\u914d\u5408\u9069\u7684\u751f\u9577\u8abf\u7bc0\u5291\u8207\u57f9\u990a\u57fa\u71df\u990a\u689d\u4ef6\uff0c\u4f7f\u539f\u751f\u8cea\u9ad4\u8868\u73fe\u51fa\u7d30\u80de\u5168\u80fd\u6027\u3002\u5982\u540c\u82b1\u7c89\u8207\u80da\u682a\u7d50\u5408\u7684\u53d7\u7cbe\u5375\u4e00\u822c\uff0c\u5728 4 \u500b\u6708\u5230 1 \u5e74\u4e4b\u9593\uff0c\u4e00\u9846\u4e0d\u5177\u6709\u7d30\u80de\u58c1\u7684\u55ae\u4e00\u539f\u751f\u8cea\u9ad4\uff0c\u91cd\u65b0\u751f\u9577\u51fa\u7d30\u80de\u58c1\uff0c\u7d93\u904e\u7d30\u80de\u5206\u88c2\u3001\u5206\u5316\u7522\u751f\u80da\u72c0\u9ad4\uff0c\u9032\u800c\u767c\u80b2\u51fa\u8396\u3001\u8449\u8207\u6839\u90e8\uff0c\u518d\u751f\u6210\u70ba\u4e00\u68f5\u4e0d\u5177\u6709\u5916\u6e90 DNA \u63d2\u5165\u3001\u4e14\u591a\u500b\u76ee\u6a19\u57fa\u56e0\u5e8f\u5217\u88ab\u7de8\u8f2f\u7684\u690d\u682a\u3002<\/p>\n<p><div id=\"attachment_21652\" style=\"width: 1034px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-21652\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_1-1024x319.png\" alt=\"\u3010\u5c08\u6b04\u3011\u57fa\u56e0\u7de8\u8f2f\u8207\u539f\u751f\u8cea\u9ad4\u518d\u751f\uff1a\u8fb2\u696d\u7684\u5d84\u65b0\u61c9\u7528\" width=\"1024\" height=\"319\" class=\"aligncenter size-large wp-image-33478\" srcset=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_1-1024x319.png 1024w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_1-300x93.png 300w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_1-768x239.png 768w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_1-1080x336.png 1080w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_1.png 1185w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><p id=\"caption-attachment-21652\" class=\"wp-caption-text\">\u25b2\u5716\u4e00\u3001(a)\u8449\u8089\u7d30\u80de\u7d93\u7e96\u7dad\u9176\u8207\u679c\u81a0\u9176\u5206\u89e3\u7d30\u80de\u58c1\u5f8c\u7372\u5f97\u6e38\u96e2\u7684\u539f\u751f\u8cea\u9ad4\u7d30\u80de\u3002(b)\u5f9e\u8449\u7247\u5206\u96e2\u51fa\u539f\u751f\u8cea\u9ad4\u7d30\u80de\u5f8c\uff0c\u5c07 RNP \u8907\u5408\u9ad4\u5c0e\u5165\u55ae\u4e00\u7d30\u80de\u767c\u751f\u57fa\u56e0\u7de8\u8f2f\uff0c\u56e0\u4e0d\u540c\u7269\u7a2e\u767c\u80b2\u901f\u5ea6\u4e0d\u540c\uff0c\u5728\u4e0d\u540c\u751f\u9577\u8abf\u7bc0\u5291\u57f9\u990a\u57fa\u57f9\u990a 4-12 \u500b\u6708\u53ef\u518d\u751f\u70ba\u4e0d\u5177\u6709\u5916\u6e90\u57fa\u56e0\u5c0e\u5165\u67d3\u8272\u9ad4\u7684\u57fa\u56e0\u7de8\u8f2f\u690d\u682a <sup>3<\/sup>\u3002<\/p><\/div><br \/>\n&nbsp;<\/p>\n<p>\u81fa\u7063\u5728\u7d44\u7e54\u57f9\u990a\u7684\u6280\u8853\u975e\u5e38\u5148\u9032\u8207\u767c\u9054\u3002\u76ee\u524d\u5305\u62ec\u862d\u82b1\u53ca\u9999\u8549\u7684\u7a2e\u82d7\u7686\u662f\u4f7f\u7528\u7d44\u7e54\u57f9\u990a\u7684\u6280\u8853\u751f\u7522\u3002\u5728\u9019\u4e9b\u57fa\u790e\u4e4b\u4e0b\uff0c\u6211\u5011\u5be6\u9a57\u5ba4\u65bc 2018 \u5e74\u5229\u7528\u83f8\u8349\u5efa\u7acb\u539f\u751f\u8cea\u9ad4\u57fa\u56e0\u7de8\u8f2f\u8207\u518d\u751f\u6280\u8853\u5f8c\uff0c\u7a4d\u6975\u958b\u767c\u4e0d\u540c\u7269\u7a2e\u7684\u539f\u751f\u8cea\u9ad4\u7d30\u80de\u8a98\u5c0e\u8207\u518d\u751f\u57f9\u990a\u689d\u4ef6\u3002\u81f3\u4eca\u5df2\u7d93\u6210\u529f\u5c07\u9019\u500b\u6280\u8853\u61c9\u7528\u5728\u963f\u62c9\u4f2f\u82a5\u3001\u5713\u8449\u83f8\u8349 <sup>4<\/sup>\u8207\u8553\u85b9\u5c6c\u6a21\u5f0f\u690d\u7269\uff1b\u7955\u9b6f\u756a\u8304\uff08\u756a\u8304\u91cd\u8981\u6297\u75c5\u6297\u9006\u5883\u80b2\u7a2e\u89aa\u672c\uff09<sup>5<\/sup>\uff1b\u7518\u85cd\u3001\u9752\u82b1\u83dc\u3001\u82b1\u6930\u83dc\uff08\u91cd\u8981\u852c\u83dc\u4f5c\u7269\uff09\uff1b\u83f8\u8349\u3001\u4e39\u53c3\u53ca\u5176\u4ed6\u8349\u85e5\u7b49\u7b49\u3002\u540c\u6642\u7d50\u5408 CRISPR\/Cas \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u91dd\u5c0d\u6297\u75c5\u57fa\u56e0\u3001\u8010\u6df9\u6c34\u57fa\u56e0\u3001\u4e8c\u7d1a\u4ee3\u8b1d\u7269\u751f\u5408\u6210\u57fa\u56e0\u7372\u5f97\u4e0d\u5177\u5916\u6e90 DNA \u63d2\u5165\u7684\u591a\u57fa\u56e0\u7de8\u8f2f\u690d\u682a\u3002\u9019\u4e9b\u57fa\u56e0\u7de8\u8f2f\u690d\u682a\u6240\u7f3a\u5931\u7684 DNA \u5e8f\u5217\u8207\u50b3\u7d71\u80b2\u7a2e\u904e\u7a0b\u4e2d\u6240\u7522\u751f\u7684\u907a\u50b3\u591a\u6a23\u6027\u76f8\u4f3c\uff0c\u672a\u4f86\u53ef\u6301\u7e8c\u61c9\u7528\u5728\u5404\u7269\u7a2e\u5206\u5b50\u80b2\u7a2e\u4e0a\uff0c\u6240\u5efa\u7acb\u7684\u6280\u8853\u64cd\u4f5c\u53c3\u6578\uff0c\u4e5f\u53ef\u4ee5\u6210\u70ba\u89aa\u6e90\u76f8\u8fd1\u7269\u7a2e\u7684\u7814\u7a76\u57fa\u790e\u3002<\/p>\n<p><div id=\"attachment_21652\" style=\"width: 1034px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-21652\" src=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_2-1024x461.jpg\" alt=\"\u3010\u5c08\u6b04\u3011\u57fa\u56e0\u7de8\u8f2f\u8207\u539f\u751f\u8cea\u9ad4\u518d\u751f\uff1a\u8fb2\u696d\u7684\u5d84\u65b0\u61c9\u7528\" width=\"1024\" height=\"461\" class=\"aligncenter size-large wp-image-33479\" srcset=\"https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_2-1024x461.jpg 1024w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_2-300x135.jpg 300w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_2-768x346.jpg 768w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_2-1536x691.jpg 1536w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_2-1080x486.jpg 1080w, https:\/newsletter.sinica.edu.tw\/wp-content\/uploads\/2023\/12\/3-2_2.jpg 1920w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><p id=\"caption-attachment-21652\" class=\"wp-caption-text\">\u25b2\u5716\u4e8c\u3001(a)\u5229\u7528\u539f\u751f\u8cea\u9ad4\u518d\u751f\u53ca RNP \u6280\u8853\u9032\u884c\u79d8\u9b6f\u756a\u8304\u57fa\u56e0\u5e73\u53f0\u7684\u5efa\u7acb\uff0c\u85c9\u7531\u6297\u75c5\u57fa\u56e0\u7684\u7de8\u8f2f\uff0c\u4ee5\u4e86\u89e3\u5176\u6297\u75c5\u6a5f\u5236\u3002<br \/>\n(b)\u76f8\u95dc\u7684\u7814\u7a76\u6210\u679c\u7372\u9078\u70ba\u690d\u7269\u9818\u57df\u91cd\u8981\u671f\u520a\u300a<em>Plant Physiology<\/em>\u300b\u7684\u5c01\u9762\u3002<\/p><\/div><br \/>\n&nbsp;<\/p>\n<hr>\n<p><sup>1<\/sup> Synthesis Report of The IPCC Sixth Assessment Report(AR6), <a href=\"https:\/report.ipcc.ch\/ar6syr\/pdf\/IPCC_AR6_SYR_SPM.pdf\" rel=\"noopener\" target=\"_blank\">https:\/report.ipcc.ch\/ar6syr\/pdf\/IPCC_AR6_SYR_SPM.pdf<\/a><br \/>\n<sup>2<\/sup> Jiang F, Doudna JA. CRISPR-Cas9 Structures and Mechanisms. Annu Rev Biophys. 2017 May 22;46:505-529. doi: 10.1146\/annurev-biophys-062215-010822.<br \/>\n<sup>3<\/sup> Lin CS, Hsu CT, Yang LH, Lee LY, Fu JY, Cheng QW, Wu FH, Hsiao HC, Zhang Y, Zhang R, Chang WJ, Yu CT, Wang W, Liao LJ, Gelvin SB, Shih MC. Application of protoplast technology to CRISPR\/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration. Plant Biotechnol J. 2018 Jul;16(7):1295-1310. doi: 10.1111\/pbi.12870.<br \/>\n<sup>4<\/sup> Hsu CT, Yuan YH, Lin YC, Lin S, Cheng QW, Wu FH, Sheen J, Shih MC, Lin CS. Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration. CRISPR J. 2021 Oct;4(5):752-760. doi: 10.1089\/crispr.2021.0045.<br \/>\n<sup>5<\/sup> Lin CS, Hsu CT, Yuan YH, Zheng PX, Wu FH, Cheng QW, Wu YL, Wu TL, Lin S, Yue JJ, Cheng YH, Lin SI, Shih MC, Sheen J, Lin YC. DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato <em>Solanum peruvianum<\/em> using protoplast regeneration. Plant Physiol. 2022 Mar 28;188(4):1917-1930. doi: 10.1093\/plphys\/kiac022. PMID: 35088855; PMCID: PMC8968427.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; \u4eba\u985e\u81ea\u4e00\u842c\u5169\u5343\u5e74\u524d\u958b\u59cb\u99b4\u5316\u3001\u9078\u64c7\u9069\u5408\u683d\u57f9\u7684\u7ce7\u98df\u4f5c\u7269\uff0c\u4e26\u4e14\u900f\u904e\u6230\u722d\u3001\u90e8\u843d\u9077\u5f99\u4ee5\u53ca\u5546\u696d\u884c\u70ba\uff0c\u5c0b\u627e\u66f4\u5408 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":33560,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[991,983],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/33473"}],"collection":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/comments?post=33473"}],"version-history":[{"count":7,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/33473\/revisions"}],"predecessor-version":[{"id":33483,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/posts\/33473\/revisions\/33483"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media\/33560"}],"wp:attachment":[{"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/media?parent=33473"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/categories?post=33473"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/newsletter.sinica.edu.tw\/wp-json\/wp\/v2\/tags?post=33473"}],"curies":[{"name":"wp","href":"https:\/api.w.org\/{rel}","templated":true}]}}