{"id":12691,"date":"2023-11-02T00:02:13","date_gmt":"2023-11-01T16:02:13","guid":{"rendered":"https:\/\/newsletter.sinica.edu.tw/en\/?p=12691"},"modified":"2024-03-13T09:11:05","modified_gmt":"2024-03-13T01:11:05","slug":"an-atp-independent-role-of-prp16-in-promoting-aberrant-splicing","status":"publish","type":"post","link":"https:\/\/newsletter.sinica.edu.tw/en\/12691\/","title":{"rendered":"An ATP-independent Role of Prp16 in Promoting Aberrant Splicing"},"content":{"rendered":"<p>Most eukaryotic genes contain sequences that interrupt the genes\u2019 continuity yet are not translated into proteins. These sequences are removed at the RNA level in a process termed RNA splicing, which occurs on a large ribonucleoprotein complex known as the spliceosome. The DExD\/H-box RNA helicase Prp16 plays a pivotal role in the splicing process. Following the branching reaction, it utilizes its ATP-dependent functions to facilitate exon ligation by displacing step-one factors, Cwc25 and Yju2, from the spliceosome\u2019s catalytic center, thus enabling the positioning of the 3\u2019 splice site to the catalytic center. Additionally, Prp16 is well known for its role in proofreading the 5\u2019 splice site and the branch site of pre-mRNA, a function that requires ATP. A team led by Distinguished Research Fellow Soo-Chen Cheng at the Institute of Molecular Biology, Academia Sinic has unveiled a paradoxical role for Prp16 in splicing of pre-mRNA with mutations at splice sites, resulting in a slower splicing reaction. In such cases, Prp16 exhibits an ATP-independent activity by stabilizing the binding of Cwc25, thereby enhancing the branching reaction and leading to aberrant selection of splice sites. This seemly contradictory function of Prp16 raises questions about its role in proofreading splice sites. Nevertheless, it underscores Prp16\u2019s consistent role in propelling the splicing pathway forward. In normal pre-mRNA, Prp16 destabilizes Cwc25 to facilitate exon ligation, whereas for mutant pre-mRNA, it stabilizes Cwc25 to promotes the branching reaction. These discoveries change the current view of splicing fidelity control, leading to the paper\u2019s recognition as Breakthrough Article by NAR.<\/p>\n<p>Article link: <a href=\"https:\/\/academic.oup.com\/nar\/advance-article\/doi\/10.1093\/nar\/gkad861\/7321994?login=true\" rel=\"noopener\" target=\"_blank\">https:\/\/academic.oup.com\/nar\/advance-article\/doi\/10.1093\/nar\/gkad861\/7321994?login=true<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/10\/3-1-2.jpg\" alt=\"An ATP-independent Role of Prp16 in Promoting Aberrant Splicing\" width=\"900\" height=\"600\" class=\"aligncenter size-full wp-image-12692\" srcset=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/10\/3-1-2.jpg 900w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/10\/3-1-2-300x200.jpg 300w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/10\/3-1-2-768x512.jpg 768w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Most eukaryotic genes contain sequences that interrupt the genes\u2019 continuity yet are not translated into proteins. These sequences are removed at the RNA level in a process termed RNA splicing, which occurs on a large ribonucleoprotein complex known as the spliceosome. The DExD\/H-box RNA helicase Prp16 plays a pivotal role in the splicing process. Following [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":12693,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[533],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12691"}],"collection":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/comments?post=12691"}],"version-history":[{"count":1,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12691\/revisions"}],"predecessor-version":[{"id":13129,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12691\/revisions\/13129"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media\/12693"}],"wp:attachment":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media?parent=12691"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/categories?post=12691"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/tags?post=12691"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}