{"id":12584,"date":"2023-09-21T00:03:17","date_gmt":"2023-09-20T16:03:17","guid":{"rendered":"https:\/\/newsletter.sinica.edu.tw/en\/?p=12584"},"modified":"2024-03-13T09:19:02","modified_gmt":"2024-03-13T01:19:02","slug":"structural-convergence-endows-nuclear-transport-receptor-kap114p-with-a-transcriptional-repressor-function-toward-tata-binding-protein","status":"publish","type":"post","link":"https:\/\/newsletter.sinica.edu.tw/en\/12584\/","title":{"rendered":"Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein"},"content":{"rendered":"
The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-\u03b2 (Kap-\u03b2) in yeast, and various regulatory factors. Previous studies showed that Kap114p, a Kap-\u03b2 that mediates nuclear import of yeast TBP (yTBP), modulates yTBP-dependent transcription. However, how Kap114p associates with yTBP to exert its multifaceted functions has remained elusive. The team led by Dr. Kuo-Chiang Hsia (Institute of Molecular Biology, Academia Sinica) and Dr. Wei-Yi Chen (Institute of Biochemistry and Molecular Biology, College of Life Sciences, National Yang Ming Chiao Tung University) presents a cryo-EM structure of Kap114p, one of the Kap-\u03b2s, in complex with TATA box binding protein and reveals a non-canonical function beyond nuclear transport that modulates TBP-dependent transcription. This study has been published in in the journal Nature Communications<\/em>.<\/p>\n