{"id":12548,"date":"2023-09-07T00:02:14","date_gmt":"2023-09-06T16:02:14","guid":{"rendered":"https:\/\/newsletter.sinica.edu.tw/en\/?p=12548"},"modified":"2024-03-13T09:19:02","modified_gmt":"2024-03-13T01:19:02","slug":"ai-accelerates-protein-design-and-enhances-enzyme-activity","status":"publish","type":"post","link":"https:\/\/newsletter.sinica.edu.tw/en\/12548\/","title":{"rendered":"AI Accelerates Protein Design and Enhances Enzyme Activity"},"content":{"rendered":"<p>Unlocking the potential of protein activation has historically posed challenges, necessitating extensive screening and biochemical assays. Dr. Kuen-Phon Wu at the Institute of Biological Chemistry has led a team to demonstrate a paradigm shift. By integrating the advanced deep learning tool, ProteinMPNN, they have engineered ubiquitin into a potent allosteric activator tailored for Rsp5 E3 ligase. The entire process was fully automated from protein designs to sample preparation to functional assays took only three months. This milestone heralds a new era, where AI-driven protein design obviates the need for exhaustive experimental screening and validation. This study has been published in in the journal <em>ACS Synthetic Biology<\/em> (August 2023).<\/p>\n<p>Article link: <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acssynbio.3c00042\" rel=\"noopener\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acssynbio.3c00042<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2.jpg\" alt=\"AI Accelerates Protein Design and Enhances Enzyme Activity\" width=\"1800\" height=\"1202\" class=\"aligncenter size-full wp-image-12549\" srcset=\"https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2.jpg 1800w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-300x200.jpg 300w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-1024x684.jpg 1024w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-768x513.jpg 768w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-1536x1026.jpg 1536w, https:\/\/newsletter.sinica.edu.tw/en\/wp-content\/uploads\/2023\/09\/3-2-1080x721.jpg 1080w\" sizes=\"(max-width: 1800px) 100vw, 1800px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Unlocking the potential of protein activation has historically posed challenges, necessitating extensive screening and biochemical assays. Dr. Kuen-Phon Wu at the Institute of Biological Chemistry has led a team to demonstrate a paradigm shift. By integrating the advanced deep learning tool, ProteinMPNN, they have engineered ubiquitin into a potent allosteric activator tailored for Rsp5 E3 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":12550,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[533],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12548"}],"collection":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/comments?post=12548"}],"version-history":[{"count":2,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12548\/revisions"}],"predecessor-version":[{"id":13138,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/posts\/12548\/revisions\/13138"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media\/12550"}],"wp:attachment":[{"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/media?parent=12548"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/categories?post=12548"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/newsletter.sinica.edu.tw/en\/wp-json\/wp\/v2\/tags?post=12548"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}